Classification and Identification of Plant Pathogenic Pseudomonas species by REP-PCR Derived Genetic Fingerprints

Stead, D. E.; Simpkins, S. A.; Weller, Simon A.; Hennessy, J.; Aspin, A.; Stanford, H.; Smith, N. C.; Elphinstone, J. G.

doi:10.1007/978-94-017-0133-4_45

Cited by 11 publications

(10 citation statements)

References 4 publications

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“…WH 6/01, WM 2/02 and WW 4/02) the DNA banding patterns and disease symptoms were different. These results agree with the rep‐PCR and AFLP results of Stead et al . (2003) and Manceau & Brin (2003), respectively, demonstrating the genetic heterogeneity in virulence in P. syringae pv.…”

Section: Discussionsupporting

confidence: 93%

“…Such results have previously been demonstrated and it has been suggested that P. syringae pv. lachrymans is genetically heterogeneous (Manceau & Brin, 2003; Stead et al ., 2003).…”

Section: Discussionmentioning

confidence: 99%

“…lachrymans and P. s. pv. syringae were heterogeneous (Stead et al ., 2003). Three different rep‐PCR clusters were identified among strains of P. s. pv.…”

Section: Introductionmentioning

confidence: 99%

“…phaseolicola , ii) two strains similar in banding patterns to P. syringae pv. syringae , and iii) a unique strain (Stead et al ., 2003). This diversity within P. syringae pv.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Pseudomonas syringae pv. lachrymans strains isolated from cucumber leaves collected in Poland

et al. 2006

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Several strains of Pseudomonas syringae pathovar (pv.) lachrymans and related bacterial pathogens were isolated from cucumber ( Cucumis sativus ) leaves collected in central and southern Poland in 2001 and 2002. Twenty five original strains, together with five reference strains of P. syringae pv. lachrymans , pv. syringae and pv. tomato , were genetically characterized by PCR-RFLP (polymerase chain reaction − restriction fragment length polymorphism), ADSRRS (amplification of DNA fragments surrounding rare restriction sites), and PCR-MP (PCR − melting profiles) fingerprinting techniques. Genetic similarity analyses of the PCR-RFLP and ADSRRS fingerprints showed that strains of P. syringae pv. lachrymans form distinct clusters. The results also indicated that the ADSRRS and the PCR-MP fingerprinting techniques may serve as more efficient tools for evaluating genetic similarity among pathovars and strains of P. syringae than PCR-RFLP. The 25 strains showed diverse pathogenicity to cucumber seedlings and biochemical tests were varied. The syrB gene was identified in four cucumber strains, characterized as P. syringae pv. syringae .

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Section: Discussionsupporting

confidence: 93%

“…Such results have previously been demonstrated and it has been suggested that P. syringae pv. lachrymans is genetically heterogeneous (Manceau & Brin, 2003; Stead et al ., 2003).…”

Section: Discussionmentioning

confidence: 99%

“…lachrymans and P. s. pv. syringae were heterogeneous (Stead et al ., 2003). Three different rep‐PCR clusters were identified among strains of P. s. pv.…”

Section: Introductionmentioning

confidence: 99%

“…phaseolicola , ii) two strains similar in banding patterns to P. syringae pv. syringae , and iii) a unique strain (Stead et al ., 2003). This diversity within P. syringae pv.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Pseudomonas syringae pv. lachrymans strains isolated from cucumber leaves collected in Poland

et al. 2006

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“…Gardan et al (34) grouped 56 pathovars of P. syringae and relatives into six genomospecies based on total DNA-DNA hybridization, and Stead et al (87) classified 500 strains using repetitive extragenic palindromic (REP)-PCR fingerprints. Gardan et al (34) grouped 56 pathovars of P. syringae and relatives into six genomospecies based on total DNA-DNA hybridization, and Stead et al (87) classified 500 strains using repetitive extragenic palindromic (REP)-PCR fingerprints.…”

Section: Taxonomic Relationshipsmentioning

confidence: 99%

Pseudomonas syringaeDiseases of Fruit Trees: Progress Toward Understanding and Control

et al. 2007

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Diseases of fruit trees caused by pathovars of Pseudomonas syringae van Hall are of major concern in fruitproducing areas worldwide, are exceedingly difficult to control, and result in significant economic losses. The pathogen has the ability to kill both young and older trees. Systemic infection and death of young trees is a perennial problem in nurseries, and canker development leading to the girdling and death of scaffold limbs and entire trees is a common event that can lead to the rapid demise of older orchards. For example, bacterial canker of plum caused by P. syringae pv. syringae causes annual tree mortality rates as high as 30% in Germany (43), and bacterial canker of hazelnut has resulted in the mortality of large numbers of trees in Italy and other European countries (77). Yield losses due to lesions on fruit are typically more sporadic in occurrence and variety dependent, but significant yearly losses have been reported in some instances. Finally, the pathogen's trait of ice nucleation activity also could exacerbate the importance of frost injury as a predisposing factor for infection. Frost damage in commercial orchards is a sporadic problem that facilitates colonization and the initiation of pathogenesis for these opportunistic pathogens.P. syringae and its close relatives cause diseases of monocots, herbaceous dicots, and woody dicots, and these pathogens utilize an impressive array of virulence factors such as effectors, toxins, and phytohormones to incite disease symptoms. Aspects of the systematics, ecology, and genetics of P. syringae have been reviewed and discussed by many authors (cited literature examples: 6,27,44,47,52). Extensive recent research efforts have focused on Pseudomonas diseases of herbaceous plants, and our understanding of P. syringae-host interactions has been facilitated by genome sequencing, comparative genomics, and functional studies (cited literature examples: 21,31,96).Our understanding of and ability to manage P. syringae diseases of fruit trees is relevant to our understanding of P. syringae diseases of herbaceous hosts, and vice versa. The exciting new insights into host-pathogen interactions from the model systems can give us clues on how to pro-ceed with the less genetically pliable fruit tree systems. Studies of the fruit tree systems, and comparisons with the model systems, can provide insights into the interactions of P. syringae strains with woody hosts and address relevant questions, such as: What is the role of type III secretion in infection of woody tissues? What virulence traits enable the colonization of woody tissue by P. syringae? Are there differences in the roles of toxins in diseases of woody hosts and herbaceous hosts? How did some pathogens evolve the ability to induce galls in woody hosts? Can resistance mechanisms characterized in the model-system herbaceous hosts be exploited in fruit trees?The objectives of this article are (i) to provide an overview of several Pseudomonas diseases of fruit trees, (ii) to discuss the current and emerging unde...

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Current Status of Some New and Some Old Plant Pathogenic Pseudomonads

Stead¹,

Stanford²,

Aspin³

et al. 2003

Pseudomonas Syringae and Related Pathogens

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Classification and Identification of Plant Pathogenic Pseudomonas species by REP-PCR Derived Genetic Fingerprints

Cited by 11 publications

References 4 publications

Genetic diversity of Pseudomonas syringae pv. lachrymans strains isolated from cucumber leaves collected in Poland

Genetic diversity of Pseudomonas syringae pv. lachrymans strains isolated from cucumber leaves collected in Poland

Pseudomonas syringaeDiseases of Fruit Trees: Progress Toward Understanding and Control

Current Status of Some New and Some Old Plant Pathogenic Pseudomonads

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