Classification of 1,198 Cases of Bovine Lymphoma Using the National Cancer Institute Working Formulation for Human Non-Hodgkin's Lymphomas

Abstract

Abstract. A retrospective histologic study was made of 1,198 cases of bovine lymphoma using the National Cancer Institute Working Formulation for human non-Hodgkin's lymphoma. This classification scheme was found to be readily applicable to bovine lymphoma. Most of the cell types described in the National Cancer Institute Working Formulation occurred in this series of bovine lymphomas, but the distribution of cell types varied markedly compared to that of human beings. Eighty-nine percent (1,067/1,198) of bovine lymphomas were high-grade tumors. The diffuse large cell type and its cleaved variant comprised 65.9% of all bovine lymphomas. Similar to the dog, but in marked contrast to human beings where at least 34% of non-Hodgkin's lymphomas were follicular, follicular tumors were found to be extremely rare in cattle (0.3% or 411,198). The prevalence of cell types varied significantly between the enzootic and sporadic lymphomas. The cleaved variant ofthe diffuse large cell type constituted 38% (406/1,072) of enzootic lymphomas versus 14% (1 8/126) of sporadic lymphomas. The mitotic index (100 x oil immersion field, 175 pm in diameter) of enzootic lymphomas (3.72 k 0.06, mean k standard error) was significantly greater than the mitotic index of sporadic lymphomas (2.82 f 0.17). We concluded that the cleaved variant of the diffise large cell type with high mitotic index is characteristic of enzootic lymphoma. This Characteristic high-grade cell type may be a consequence of the viral etiology of the enzootic form of bovine lymphoma.

Cytology of B Cell Lymphomas in Cattle Infected with Bovine Leukosis Virus

“…In previous reports of enzootic bovine leukosis 20,21 , the cytological classifications were primarily based on tumor cell size, which can cause difficulties in classifying enzootic bovine leukosis from other leukoses. Similar to the reports for pleomorphic B cell lymphoma 1,9,13. , in this study the main cytological findings from cases 1-16 were pleomorphism and atypia, which are not so prominent in other histological types of lymphoid neoplasms 1,2,[6][7][8][9][10]13,14,17,18 .…”

“…Numerous bovine lymphoid neoplasms were classified using the National Cancer Institute Working Formulation for human lymphomas. Via this classification system the cleaved variant of the diffuse large cell type has been shown to constitute 38% of enzootic lymphomas versus 14% of sporadic lymphomas 20 . Yin et al 21 examined 47 cases of enzootic and sporadic leukosis, which were divided into four histological types: lymphocytic; well-differentiated prolymphocytic; poorly-differentiated prolymphocytic; and histiocytic.…”

Immunophenotypic and Histologic Classification of 50 Cases of Feline Gastrointestinal Lymphoma

“…T-cell lymphoma tended to predominate in the small intestine, and this finding is supported by a recent report that identified the origin of these tumors as the diffuse mucosaassociated lymphoid tissue of the small intestine. 26 Since its original development for the characterization of human non-Hodgkin's lymphoma, the NCI WF 1 has been used successfully to characterize lymphomas in cattle, [43][44][45] dogs, 5 and cats 40 and to predict the clinical course of disease (indolent, intermediate-grade, and high-grade or rapidly progressive 1 ) with reasonable accuracy in these species. 3 Although the NCI WF is relatively simple to apply to histologic sections through a dichotomous algorithm, 5 the most accurate predictive models, particularly for tumors of small (mature) cell types, requires an integration of both mitotic rate and cell type.…”

Classificação de 86 casos de linfoma em bovinos de acordo com a Working Formulation (WF) of Non-Hodgkin's Lymphomas for Clinical Usage e a Revised European-American Classification of Lymphoid Neoplasms (REAL)

“…A WF foi proposta em meados da década de 1980, não como uma nova classificação, mas como um meio de traduzir os diversos sistemas em uso na época (método de Rappaport, sistema Kiel e classificação de Lukes-Collins) e com o intuito de facilitar as comparações clínicas e os tratamentos terapêuticos (Parodi 2001). Um grande estudo de classificação histológica de linfoma em bovinos realizado no Canadá (Vernau et al 1992), demonstrou a aplicabilidade da WF nesta espécie. Nesse, quase dois terços dos tumores linfoides de bovinos adultos (possivelmente todos enzoóticos) foram classificados como linfomas difusos de grandes células clivadas e linfomas difusos de grandes células não clivadas, ambos os tipos histológicos com prevalências equivalentes (35,5% e 30,6%, respectivamente).…”