Classification of 1-methylcyclopropene treated apples by fluorescence fingerprint using partial least squares discriminant analysis with stepwise selectivity ratio variable selection method

Trivittayasil, Vipavee; Tsuta, Mizuki; Kasai, S.; Matsuo, Yosuke; Sekiyama, Yasuyo; Shoji, Toshihiko; Aiyama, Ryoko; Kokawa, Mito; Sugiyama, Junichi

doi:10.1016/j.chemolab.2018.02.004

Cited by 12 publications

(2 citation statements)

References 34 publications

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“…Two analyses were done, the first one with markers at baseline and the second one with the variations of the markers after treatment (Δ: POST minus PRE values). PLS-DA can handle both, multicollinearity problem and datasets with the number of variables higher than that of samples [24]. To choose the optimal number of variables for each PLS-DA, we use the estimation of the misclassification error rate using leave-one-out or stratified cross-validation [23].…”

Section: Discussionmentioning

confidence: 99%

Peripheral changes in immune cell populations and soluble mediators after anti-PD-1 therapy in non-small cell lung cancer and renal cell carcinoma patients

Juliá

Mandó

Rizzo

et al. 2019

Cancer Immunol Immunother

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Patients with non-small cell lung cancer (NSCLC) and renal cell carcinoma (RCC) have shown benefit from anti-PD-1 therapies. However, not all patients experience tumor shrinkage, durable responses or prolonged survival, demonstrating the need to find response markers. In blood samples from NSCLC and RCC patients obtained before and after anti-PD-1 treatment, we studied leukocytes by complete blood cell count, lymphocyte subsets using flow cytometry and plasma concentration of nine soluble mediators, in order to find predictive biomarkers of response and to study changes produced after anti-PD-1 therapy. In baseline samples, discriminant analysis revealed a combination of four variables that helped differentiate stable disease-response (SD-R) from progressive disease (PD) patients: augmented frequency of central memory CD4 + T cells and leukocyte count was associated with response while increased percentage of PD-L1 + natural killer cells and naïve CD4 + T cells was associated with lack of response. After therapy, differential changes between responders and non-responders were found in leukocytes, T cells and TIM-3 + T cells. Patients with progressive disease showed an increase in the frequency of TIM-3 expressing CD4 + and CD8 + T cells, whereas SD-R patients showed a decrease in these subsets. Our findings indicate that a combination of immune variables from peripheral blood (PB) could be useful to distinguish response groups in NSCLC and RCC patients treated with anti-PD-1 therapy. Frequency of TIM-3 + T cells showed differential changes after treatment in PD vs SD-R patients, suggesting that it may be an interesting marker for monitoring progression during therapy.

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Section: Discussionmentioning

confidence: 99%

Peripheral changes in immune cell populations and soluble mediators after anti-PD-1 therapy in non-small cell lung cancer and renal cell carcinoma patients

Juliá

Mandó

Rizzo

et al. 2019

Cancer Immunol Immunother

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“…The advantage of the FF technique over conventional fluorescence spectroscopy is that it includes the signals of many fluorescent constituents existing in the sample. Many studies using the FF technique to evaluate various food stuffs, such as olive oil (Guimet et al 2006), beer (Sikorska et al 2008), cheese (M. Kokawa et al 2015), soymilk (Mito Kokawa et al 2017, and apples (Trivittayasil et al 2018), have been reported. Trivittayasil et al (2017) reported that a model for estimating the active oxygen-scavenging ability of peach extract was constructed from the fluorescence patterns of epicatechin and procyanidin (Trivittayasil et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of Apple Juice Manufacturing Processes on Procyanidin Concentration and Nondestructive Analysis by Fluorescence Fingerprint

Okino

Kokawa

Islam

et al. 2021

Food Bioprocess Technol

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Procyanidins are one of the main polyphenols in apple fruit. In this study, we aimed to increase the amount of extractable procyanidins by micro-wet milling (MWM), a novel milling process that can wet-mill foods to micrometer scale, in addition to commonly applied apple juice manufacturing processes. The effects of milling, pasteurization, centrifugation, and enzymatic treatment on extractable procyanidin concentration were investigated, and MWM was shown to increase the procyanidin concentration by 16.7% compared with mixer milling. Conversely, other processes such as pasteurization, centrifugation, and enzymatic treatment decreased the procyanidin concentration in apple juice. Since procyanidin concentrations in apple juice are also affected by the variability between individual apples, we attempted to nondestructively estimate the procyanidin concentration at each process of apple juice manufacturing by obtaining the fluorescence fingerprint (FF). The FFs are a set of fluorescence spectra acquired at consecutive excitation wavelengths. Partial least-squares regression was used to estimate the procyanidin concentrations of apple juice from the FFs, and the most accurate model was able to estimate procyanidin concentration at the quality control analysis level.

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Estimation of ‘Hass’ Avocado (Persea americana Mill.) Ripeness by Fluorescence Fingerprint Measurement

Kokawa

Hashimoto

et al. 2020

Food Anal. Methods

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Avocados (Persea americana Mill.) are a climacteric fruit which ripen until after 22 harvesting, and their ripeness is an important quality attribute that determines consumer 23 liking. In this study, the ripening degree of 'Hass' avocados was evaluated non-24 destructively by measuring the skin and flesh using the fluorescence fingerprint (FF). 25 FF, also known as the Excitation emission matrix (EEM), is a set of fluorescence 26 spectra obtained at consecutive excitation wavelengths. It was found that as ripening 27 progressed, the fluorescence signal of chlorophyll A in the skin and flesh decreased 28 significantly as the hardness of the avocado flesh decreased. The hardness value was 29 estimated from the FFs of the skin and flesh using partial least-squares regression, and 30 minimum prediction errors of 2.02 N cm -2 and 2.05 N cm -2 were obtained for the 31 prediction models using FFs of the flesh and skin, respectively. Furthermore, ripeness 32 levels (unripe, ripe, and over ripe) were discriminated non-destructively from the FFs of 33 the skin with an accuracy of 90% for the validation dataset. The measurement and 34 analysis technique demonstrated in this study is rapid and accurate, and can contribute 35 to supplying uniform agricultural products to consumers. 36 37 Keywords: 38 Excitation emission matrix (EEM); Partial least-squares regression; discrimination 39 analysis; texture measurement; polyphenol oxidase Avocados (Persea americana Mill.) are a climacteric fruit that ripens after harvesting. 42Maturation of the fruit occurs while the fruit is on the tree, and maturation is characterized 43 by an increase in the fat content and a concurrent decrease in the water content (Clark et 44 al. 2007). Interestingly, the avocado fruit does not ripen while it is on the tree and remains 45 green and hard (Paliyath et al. 2008). After harvesting, ripening occurs, characterized by 46 a change in color (green to black or purple, especially for the 'Hass' variety) and softening 47 of the flesh. 48Although there have been many reports on predicting the maturity of avocado fruit 49 non-destructively, as reviewed by Magwaza and Tesfay (2015), there have been fewer 50 reports on monitoring avocado ripeness after their harvest. Gaete-Garretón et al. (2005) 51 applied ultrasonic methods to assess avocado ripeness and showed that the adsorption 52 coefficient had a strong correlation with firmness, but this correlation was only obtained 53 from the average values of 15 fruit. Cox et al. (2004) showed that the avocado skin color 54 changes during ripening owing to a decrease in chlorophyll and an increase in 55 anthocyanins, more specifically, cyanidin-3-gluocoside. Color changes in the skin are and Technology 112: [31][32][33][34][35][36][37][38]

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Classification of 1-methylcyclopropene treated apples by fluorescence fingerprint using partial least squares discriminant analysis with stepwise selectivity ratio variable selection method

Cited by 12 publications

References 34 publications

Peripheral changes in immune cell populations and soluble mediators after anti-PD-1 therapy in non-small cell lung cancer and renal cell carcinoma patients

Peripheral changes in immune cell populations and soluble mediators after anti-PD-1 therapy in non-small cell lung cancer and renal cell carcinoma patients

Effects of Apple Juice Manufacturing Processes on Procyanidin Concentration and Nondestructive Analysis by Fluorescence Fingerprint

Estimation of ‘Hass’ Avocado (Persea americana Mill.) Ripeness by Fluorescence Fingerprint Measurement

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