Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup, O80

Siwińska, Małgorzata; Levina, Evgeniya A.; Ovchinnikova, Olga G.; Drzewiecka, Dominika; Shashkov, Alexander S.; Różalski, Antoni; Knirel, Yuriy A.

doi:10.1016/j.carres.2015.02.003

Cited by 19 publications

(14 citation statements)

References 22 publications

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“…isolates from Polish patients (Arbatsky et al, 2013;Drzewiecka et al, 2008Drzewiecka et al, , 2010Siwiń ska et al, 2015). On the other hand, multiple similarities are observed among Proteus spp.…”

Section: Discussionmentioning

confidence: 99%

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Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland

et al. 2016

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A hospitalized 73-year-old woman was infected with a Proteus mirabilis strain, 12 B-r, isolated from the place of injection of a blood catheter. Another strain, 12 B-k, recognized as Proteus genomospecies 5 or 6, was isolated from the patient's faeces, which was an example of a nosocomial infection rather than an auto-infection. Serological investigation using ELISA and Western blotting showed that strain 12 B-k from faeces belonged to the Proteus O2 serogroup. Strain 12 B-r from the wound displayed cross-reactions with several Proteus O serogroups due to common epitopes on the core or O-specific parts of the lipopolysaccharide. Studies of the isolated 12 B-r O-specific polysaccharide by NMR spectroscopy revealed its close structural similarity to that of Proteus O8. The only difference in 12 B-r was the presence of an additional GlcNAc-linked phosphoethanolamine residue, which creates a putative epitope responsible for the cross-reactivity with Pt. mirabilis O16. The new O-antigen form could appear as a result of adaptation of the bacterium to a changing environment. On the basis of the data obtained, we suggest division of the O8 serogroup into two subgroups: O8a for strains of various Proteus species that have been previously classified into the O8 serogroup, and O8a,b for Pt. mirabilis 12 B-r, where 'a' is a common epitope and 'b' is a phosphoethanolamine-associated epitope. These findings further confirm serological and structural heterogeneity of O antigens of Proteus strains isolated lately from patients in Poland.

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Section: Discussionmentioning

confidence: 99%

“…rods, giving rise to 80 O serogroups within this genus (Arbatsky et al, 2013;Knirel et al, 2011;Siwiń ska et al, 2015). However, the number of the O-antigen forms is even larger as several serogroups are divided into subgroups (serotypes), which differ slightly in chemical structure and serospecificity.…”

Section: Introductionmentioning

confidence: 99%

Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland

et al. 2016

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“…So far, there have been established 80 O-antigenic serogroups in the genus, some of them divided into subgroups [7, 67, 135], and many new O serotypes are still being discovered (D. Drzewiecka, unpublished data). The chemical structure of the sugar part of the lipopolysaccharide may play an important role in the adaptation of Proteus spp.…”

Section: Introductionmentioning

confidence: 99%

Significance and Roles of Proteus spp. Bacteria in Natural Environments

2016

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Proteus spp. bacteria were first described in 1885 by Gustav Hauser, who had revealed their feature of intensive swarming growth. Currently, the genus is divided into Proteus mirabilis, Proteus vulgaris, Proteus penneri, Proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 O-antigenic serogroups. The bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. It is postulated that intestines are a reservoir of these proteolytic organisms. Many wild and domestic animals may be hosts of Proteus spp. bacteria, which are commonly known to play a role of parasites or commensals. However, interesting examples of their symbiotic relationships with higher organisms have also been described. Proteus spp. bacteria present in soil or water habitats are often regarded as indicators of fecal pollution, posing a threat of poisoning when the contaminated water or seafood is consumed. The health risk may also be connected with drug-resistant strains sourcing from intestines. Positive aspects of the bacteria presence in water and soil are connected with exceptional features displayed by autochthonic Proteus spp. strains detected in these environments. These rods acquire various metabolic abilities allowing their adaptation to different environmental conditions, such as high concentrations of heavy metals or toxic substances, which may be exploited as sources of energy and nutrition by the bacteria. The Proteus spp. abilities to tolerate or utilize polluting compounds as well as promote plant growth provide a possibility of employing these microorganisms in bioremediation and environmental protection.

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“…Until now, OPS has been the best structurally and serologically characterized region of P. penneri LPS, which also defines the serospecificity of smooth bacterial cells. Twenty-six different OPS structures have been identified for P. penneri strains so far, among which seven are common also to the other representatives of the genus [4, 9, 10]. The P. penneri core region is less structurally diverse than OPS but in contrast to other enterobacterial LPS core regions characterized by lager structural variability.…”

Section: Introductionmentioning

confidence: 99%

“…The P. penneri classification scheme is based on the OPSs serospecificity. So far, P. penneri isolates have been classified into 17 Proteus O serogroups, among which 13 consist of these species representatives only [4, 9, 10, 13]. To have an insight into the serological specificity of both polysaccharide and oligosaccharide parts of P. penneri LPS, it is worth creating an additional scheme classifying P. penneri LPSs into serotypes of their core regions.…”

Section: Introductionmentioning

confidence: 99%

Classification of Proteus penneri lipopolysaccharides into core region serotypes

Palusiak

2016

Med Microbiol Immunol

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The frequency of P. penneri isolation from hospital patients, mostly from urine and wounds, keeps on growing, and numerous isolates are multi-drug resistant. P. penneri rods produce lipopolysaccharide (LPS), which may lead to the septic shock. Until now, O-specific polysaccharide has been the best structurally and serologically characterized region of P. penneri LPS. It is worth having an insight into the serological specificity of both poly- and oligosaccharide parts of P. penneri LPS. The P. penneri core region is less structurally diverse than OPS, but still, among other enterobacterial LPS core regions, it is characterized by structural variability. In the present study, the serological reactivity of 25 P. penneri LPS core regions was analyzed by ELISA, passive immunohemolysis and Western blot technique using five polyclonal P. penneri antisera after or without their adsorption with the respective LPSs. The results allowed the assignment of the tested strains to five new core serotypes, which together with published serological studies led to the creation of the first serotyping scheme based on LPS core reactivities of 35 P. penneri and three P. mirabilis strains. Together with the O types scheme, it will facilitate assigning Proteus LPSs of clinical isolates into appropriate O and R serotypes.

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Classification of a Proteus penneri clinical isolate with a unique O-antigen structure to a new Proteus serogroup, O80

Cited by 19 publications

References 22 publications

Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland

Immunochemical characterization of the O antigens of two Proteus strains, O8-related antigen of Proteus mirabilis 12 B-r and O2-related antigen of Proteus genomospecies 5/6 12 B-k, infecting a hospitalized patient in Poland

Significance and Roles of Proteus spp. Bacteria in Natural Environments

Classification of Proteus penneri lipopolysaccharides into core region serotypes

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