Classification of Anaerobic Gut Fungi from Herbivores with Emphasis on Rumen Fungi from Malaysia

Ho, Yin Wan; Barr, D. J. S.

doi:10.2307/3760810

Cited by 68 publications

(74 citation statements)

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“…Currently, the species are defined according to taxonomic convention by zoospore ultrastructure (Heath et al, 1983 ;Orpin & Munn, 1986 ;Webb & Theodorou, 1991). However, Ho & Barr (1995) have questioned the validity of this method as it has been known for some time that the ultrastructural characters upon which the classification is based can alter with age, method and quality of preparation.…”

Section: Abbreviationsmentioning

confidence: 99%

“…The genera Sphaeromonas and Piromonas were also renamed as Caecomyces and Piromyces to take account of their fungal affinity (Gold et al, 1988). Ho & Barr (1995) recently proposed a radical reassessment of the classification of anaerobic gut fungal species. This involves recognition of the mode of zoospore release, measurement of the diameter of the mature zoosporangium, the size of the zoospore, the length of the flagella and the position of the zoosporangium on the thallus.…”

Section: Abbreviationsmentioning

confidence: 99%

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Identification and characterization of anaerobic gut fungi using molecular methodologies based on ribosomal ITS1 and 18S rRNA The GenBank accession numbers for the sequences determined in this work are given in Methods.

et al. 2000

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The gut fungi are an unusual group of zoosporic fungi occupying a unique ecological niche, the anaerobic environment of the rumen. They exhibit two basic forms, with nuclear migration throughout the hyphal mass for polycentric species and with concentration of nuclear material in a zoosporangium for monocentric species. Differentiation between isolates of these fungi is difficult using conventional techniques. In this study, DNA-based methodologies were used to examine the relationships within and between two genera of monocentric gut fungi gathered from various geographical locations and host animals. The ribosomal ITS1 sequence from 16 mono-and 4 polycentric isolates was PCR-amplified and sequenced ; the sequences obtained were aligned with published sequences and phylogenetic analyses were performed. These analyses clearly differentiate between the two genera and reflect the previously published physiological conclusions that Neocallimastix spp. constitute a more closely related genus than the relatively divergent genus Piromyces. The analyses place two type species N. frontalis and N. hurleyensis together but, contrary to a recent suggestion in the literature, place them apart from the other agreed species N. patriciarum. In situ hybridization and slot-blotting were investigated as potential methods for detection of and differentiation between monocentric gut fungi. DNA slot-blot analysis using ribosomal sequences is able to differentiate between gut fungal genera and thus has considerable potential for use in ecological studies of these organisms.

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Section: Abbreviationsmentioning

confidence: 99%

Section: Abbreviationsmentioning

confidence: 99%

Identification and characterization of anaerobic gut fungi using molecular methodologies based on ribosomal ITS1 and 18S rRNA The GenBank accession numbers for the sequences determined in this work are given in Methods.

et al. 2000

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“…Barr (1980) erected the Spizellomycetales, from the Chytridiales based on zoospore ultrastructural characteristics. The anaerobic chytrids that inhabit the rumens and ceca of herbivorous mammals were discovered since Sparrow and comprise the order Neocallimastigales (Li and Heath 1993;Trinci et al 1994;Ho and Barr 1995).…”

Section: Chytridiomycota Chytridiomycetesmentioning

confidence: 99%

Fungal biodiversity in aquatic habitats

Shearer

Descals

Kohlmeyer³

et al. 2006

Biodivers Conserv

462

330

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“…These are regarded as the primary colonizers (1) and most active lignocellulose degraders in the biological world (23,25). Hence, their role is very critical in the digestion, especially in tropical regions, where forage is generally fibrous and of low quality (10). Taxonomically, these fungi belong to the phylum Neocallimastigomycota, class Neocallimastigomycetes, and order Neocallimastigales (9).…”

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D1/D2 Domain of Large-Subunit Ribosomal DNA for Differentiation of Orpinomyces spp

Dagar

Kumar

Mudgil

et al. 2011

Appl Environ Microbiol

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This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.

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Classification of Anaerobic Gut Fungi from Herbivores with Emphasis on Rumen Fungi from Malaysia

Cited by 68 publications

References 0 publications

Identification and characterization of anaerobic gut fungi using molecular methodologies based on ribosomal ITS1 and 18S rRNA The GenBank accession numbers for the sequences determined in this work are given in Methods.

Identification and characterization of anaerobic gut fungi using molecular methodologies based on ribosomal ITS1 and 18S rRNA The GenBank accession numbers for the sequences determined in this work are given in Methods.

Fungal biodiversity in aquatic habitats

D1/D2 Domain of Large-Subunit Ribosomal DNA for Differentiation of Orpinomyces spp

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