Classification of bentgrass (Agrostis) cultivars and accessions based on microsatellite (SSR) markers

Honig, Josh A.; Kubik, Christine; Averello, Vincenzo; Vaiciunas, Jennifer; Meyer, William A.; Bonos, Stacy A.

doi:10.1007/s10722-015-0307-6

Cited by 11 publications

(16 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is unclear if the plants were grown to an identifiable stage, and if voucher specimens were prepared (Amundsen and Warnke 2012). Indeed, Honig et al (2015) found identification errors in material of Agrostis obtained from this seed bank. On the other hand, the infraspecific variation may be real.…”

Section: Discussionmentioning

confidence: 96%

“…& Reut. (dryland bentgrass, 2 n =6 x =42, A 1 A 1 A 2 A 2 ) and A. gigantea (redtop bentgrass, 2 n =6 x =42, A 1 A 1 A 2 A 2 A 3 A 3 ) (Jones 1956a, c, b, Rotter et al 2007, 2010, Honig et al 2015). Characterizing relationships among these species has been challenging because most are allopolyploids.…”

Section: Discussionmentioning

confidence: 99%

“…They concluded the diploid A. canina is the maternal parent of the allopolyploid A. stolonifera and suggested changing its genome formula from A 1 A 1 to A 2 A 2 , in contradiction to the cytological work of K. Jones, who did not hypothesize a shared genome between A. canina (A 1 A 1 ) and A. stolonifera (A 2 A 2 A 3 A 3 ). In a phenetic study based on nuclear SSR data (Honig et al 2015), A. canina was more closely related to A. capillaris than to A. stolonifera , contrary to the ITS tree in Rotter et al (2010), whereas based on plastid SSR data, A. canina was more closely related to A. stolonifera than to A. capillaris , similar to the plastid tree in Rotter et al (2010). Honig et al (2015) suggested the close plastid relationship between A. canina and A. stolonifera may be due to introgression of the A. canina plastome into A. stolonifera , rather than A. canina being one of the parent species (the maternal one) of A. stolonifera .…”

Section: Discussionmentioning

confidence: 99%

“…In a phenetic study based on nuclear SSR data (Honig et al 2015), A. canina was more closely related to A. capillaris than to A. stolonifera , contrary to the ITS tree in Rotter et al (2010), whereas based on plastid SSR data, A. canina was more closely related to A. stolonifera than to A. capillaris , similar to the plastid tree in Rotter et al (2010). Honig et al (2015) suggested the close plastid relationship between A. canina and A. stolonifera may be due to introgression of the A. canina plastome into A. stolonifera , rather than A. canina being one of the parent species (the maternal one) of A. stolonifera . Their hypothesis of plastid introgression does not, however, explain the close relationship of A. canina and A. stolonifera in the ITS tree in Rotter et al (2010) contradicting their nuclear SSR data.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the infraspecific variation may be real. Amundsen and Warnke (2012) and Honig et al (2015) both sampled the same two accessions of A. stolonifera from the National Plant Germplasm System (PI 302902 and PI 318934) and plastid DNA in these samples did not group with other accessions of A. stolonifera . We are confident in the accuracy of these plastid data because they were generated independently from the same samples, although the possibility remains the samples are misidentified.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Molecular phylogenetics of cool-season grasses in the subtribes Agrostidinae, Anthoxanthinae, Aveninae, Brizinae, Calothecinae, Koeleriinae and Phalaridinae (Poaceae, Pooideae, Poeae, Poeae chloroplast group 1)

Saarela¹,

Bull²,

Paradis³

et al. 2017

138

View full text Add to dashboard Cite

Circumscriptions of and relationships among many genera and suprageneric taxa of the diverse grass tribe Poeae remain controversial. In an attempt to clarify these, we conducted phylogenetic analyses of >2400 new DNA sequences from two nuclear ribosomal regions (ITS, including internal transcribed spacers 1 and 2 and the 5.8S gene, and the 3’-end of the external transcribed spacer (ETS)) and five plastid regions (matK, trnL–trnF, atpF–atpH, psbK–psbI, psbA–rps19–trnH), and of more than 1000 new and previously published ITS sequences, focused particularly on Poeae chloroplast group 1 and including broad and increased species sampling compared to previous studies. Deep branches in the combined plastid and combined ITS+ETS trees are generally well resolved, the trees are congruent in most aspects, branch support across the trees is stronger than in trees based on only ITS and fewer plastid regions, and there is evidence of conflict between data partitions in some taxa. In plastid trees, a strongly supported clade corresponds to Poeae chloroplast group 1 and includes Agrostidinae p.p., Anthoxanthinae, Aveninae s.str., Brizinae, Koeleriinae (sometimes included in Aveninae s.l.), Phalaridinae and Torreyochloinae. In the ITS+ETS tree, a supported clade includes these same tribes as well as Sesleriinae and Scolochloinae. Aveninae s.str. and Sesleriinae are sister taxa and form a clade with Koeleriinae in the ITS+ETS tree whereas Aveninae s.str. and Koeleriinae form a clade and Sesleriinae is part of Poeae chloroplast group 2 in the plastid tree. All species of Trisetum are part of Koeleriinae, but the genus is polyphyletic. Koeleriinae is divided into two major subclades: one comprises Avellinia, Gaudinia, Koeleria, Rostraria, Trisetaria and Trisetum subg. Trisetum, and the other Calamagrostis/Deyeuxia p.p. (multiple species from Mexico to South America), Peyritschia, Leptophyllochloa, Sphenopholis, Trisetopsis and Trisetum subg. Deschampsioidea. Graphephorum, Trisetum cernuum, T. irazuense and T. macbridei fall in different clades of Koeleriinae in plastid vs. nuclear ribosomal trees, and are likely of hybrid origin. ITS and matK trees identify a third lineage of Koeleriinae corresponding to Trisetum subsect. Sibirica, and affinities of Lagurus ovatus with respect to Aveninae s.str. and Koeleriinae are incongruent in nuclear ribosomal and plastid trees, supporting recognition of Lagurus in its own subtribe. A large clade comprises taxa of Agrostidinae, Brizinae and Calothecinae, but neither Agrostidinae nor Calothecinae are monophyletic as currently circumscribed and affinities of Brizinae differ in plastid and nuclear ribosomal trees. Within this clade, one newly identified lineage comprises Calamagrostis coarctata, Dichelachne, Echinopogon (Agrostidinae p.p.) and Relchela (Calothecinae p.p.), and another comprises Chascolytrum (Calothecinae p.p.) and Deyeuxia effusa (Agrostidinae p.p.). Within Agrostidinae p.p., the type species of Deyeuxia and Calamagrostis s.str. are closely related, supporting classification of Dey...

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular phylogenetics of cool-season grasses in the subtribes Agrostidinae, Anthoxanthinae, Aveninae, Brizinae, Calothecinae, Koeleriinae and Phalaridinae (Poaceae, Pooideae, Poeae, Poeae chloroplast group 1)

Saarela¹,

Bull²,

Paradis³

et al. 2017

138

View full text Add to dashboard Cite

show abstract

Genetic diversity of colonial bentgrass Agrostis capillaris based on simple sequence repeat markers and high‐resolution melt analysis with haplotype scoring

Warnke¹,

Barnaby²

2023

Crop Science

View full text Add to dashboard Cite

Colonial bentgrass (Agrostis capillaris) is a tetraploid rhizomatous bentgrass that generally exhibits improved resistance to the important fungal disease dollar spot and has more tolerance to water-deficit stress than the more widely grown creeping bentgrass (Agrostis stolonifera). Interspecific hybridization between these two species is possible and efforts to understand the genetic mechanisms of enhanced biotic and abiotic stress resistance in colonial bentgrass are proceeding. To gain a better understanding of the level of genetic diversity in cultivated colonial bentgrasses and to determine if genotypes with improved interspecific hybridization potential can be identified, nine colonial bentgrass cultivars and one creeping bentgrass cultivar were screened with 48 colonial bentgrass-derived simple sequence repeat markers.Twenty-one primer pairs were found to produce reliable amplification, and these markers were scored using high-resolution melt analysis to generate haplotypes that were used to evaluate cultivar relationships. The nine colonial bentgrass cultivars could be placed into two groups and overall showed limited levels of diversity. The creeping bentgrass cultivar was distinct and individual genotypes within each species exhibited haplotypes more common in the alternate species, suggesting that these markers may be useful for selecting genotypes with enhanced interspecific hybridization potential.

show abstract

Characterization of the complete chloroplast genome and development of molecular markers of Salix

Wang,

Guo,

Zhou

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Salix , an economically and ecologically multifunctional tree species widely distributed in China, encompasses five ornamental species sequenced in this study, which are highly beneficial for plant phytoremediation due to their ability to absorb heavy metals. This research utilized high-throughput sequencing to acquire chloroplast genome sequences of Salix , analyzing their gene composition and structural characteristics, identifying potential molecular markers, and laying a foundation for Salix identification and resource classification. Chloroplast DNA was extracted from the leaves of Salix argyracea , Salix dasyclados , Salix eriocephala , Salix integra ‘Hakuro Nishiki’, and Salix suchowensis using an optimized CTAB method. Sequencing was conducted on the Illumina NovaSeq PE150 platform, and bioinformatics tools were employed to compare the structural features and variations within the chloroplast genomes of the Salix . Analysis revealed high similarity among the chloroplast genome sequences of the five Salix species, with a subsequent examination identifying 276, 269, 270, 273, and 273 SSR loci, respectively, along with unique simple repeat sequences in each variety. Comparison of chloroplast genomes across 22 Salix highlighted variations in regions such as matK-trnQ , ndhC-trnV , psbE-petL , rpl36-rps8 , and ndhB-rps7 , which may serve as valuable molecular markers for willow resource classification studies. In this study, chloroplast genome sequencing and structural analysis of Salix not only enhances the genetic resources of Salix but also forms a critical basis for the development of molecular markers and the exploration of interspecific phylogeny in the genus. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-79604-8.

show abstract

Classification of bentgrass (Agrostis) cultivars and accessions based on microsatellite (SSR) markers

Cited by 11 publications

References 53 publications

Molecular phylogenetics of cool-season grasses in the subtribes Agrostidinae, Anthoxanthinae, Aveninae, Brizinae, Calothecinae, Koeleriinae and Phalaridinae (Poaceae, Pooideae, Poeae, Poeae chloroplast group 1)

Molecular phylogenetics of cool-season grasses in the subtribes Agrostidinae, Anthoxanthinae, Aveninae, Brizinae, Calothecinae, Koeleriinae and Phalaridinae (Poaceae, Pooideae, Poeae, Poeae chloroplast group 1)

Genetic diversity of colonial bentgrass Agrostis capillaris based on simple sequence repeat markers and high‐resolution melt analysis with haplotype scoring

Characterization of the complete chloroplast genome and development of molecular markers of Salix

Contact Info

Product

Resources

About