Identifying birds is one of challenging role for bird watchers due to the similarity of the birds' forms/image background and the lack of experience for watchers. So, it needs a computer system based images to help birdwatchers in order to identify birds. This study aims at investigating the use of deep learning for birds' identification using convolutional neural network for extracting features from images. The investigation was performed on database contained 4340 images that collected by the paper author from Jordan. The Principal Component Analysis (was applied on layer 6 and 7, as well as on the statistical operations of merging the two layers like: average, minimum, maximum and combine of both layers. The datasets were investigated by the following classifiers: Artificial neural networks, K-Nearest Neighbor, Random Forest, Naïve Bayes and Decision Tree. Whereas, the metrics used in each classifier are: accuracy, precision, recall, and F-Measure. The results of investigation include and not limited to the following, the PCA used on the deep features does not only reduce the dimensionality, and therefore, the training/testing time is reduced significantly, but also allows for increasing the identification accuracy, particularly when using the Artificial Neural Networks classifier. Based on the results of classifiers; Artificial neural networks showed high classification accuracy (70.9908), precision (0.718), recall (0.71) and F-Measure (0.708) compared to other classifiers.