Enterococci isolatedfrom water (50 strains), clinical material (50 strains), pork products (25 strains), and other foods (25 strains) as well as 50 known strains were used to compare the abilities of four latex streptococcal grouping kits to correctly identify group D isolates. The Streptex kit (Wellcome Diagnostics) was 98.5 % accurate and easiest to interpret. The Slidex Strepto kit (Vitek Systems) and Strepslide kit (NCS Diagnostics) also were acceptable; they were 96.5% and 96.0% accurate, respectively. When the Bacto Strep Grouping kit (Difco Laboratories) was used, 99% of the group D isolates were positive for both group D and group B, including enterococcal strains that are group D-negative. 'Corresponding author; Telephone: (515) 294-8824; FAX: (515) 294-6019.