Classification of fowl adenoviruses by use of phylogenetic analysis and high-resolution melting-curve analysis of the hexon L1 gene region

Marek, Ana; Günes, Ayse; Schulz, Evelyn; Heß, Michael

doi:10.1016/j.jviromet.2010.09.019

Cited by 90 publications

(68 citation statements)

References 17 publications

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“…It also indicated differences among strains representing different serotypes [14]. This region was found in a previous study to be very useful for type-level identification of FAdVs [10,12]. Close similarities between members of the species Fowl aviadenovirus D and Fowl aviadenovirus E were described previously by Grgic et al [5] and Marek et al [11], and, more recently, by Günes et al [4].…”

Section: Discussionsupporting

confidence: 54%

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Niczyporuk

2015

Arch Virol

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Fowl adenoviruses (FAdVs) are widely distributed in chickens in Poland and throughout the world. FAdV infections have been reported in the United States, Australia, Europe, and the Mediterranean basin. Detection of FAdVs strains is very important from the epidemiological point of view and for monitoring disease outbreaks and developing strategies for vaccine development. Several molecular epidemiology and phylogenetic studies have been performed, but the results obtained are still limited, because FAdV strains, even of the same serotype, have very diverse characteristics. Some strains are pathogenic and some are nonpathogenic. This report describes the successful isolation of 96 FAdV field strains from chickens in Poland. A PCR assay specific for the L1 loop region of the hexon gene was conducted, and the products were subjected to sequence analysis. The sequences were analysed using BLAST and Geneious 6.0 software and compared to adenovirus field and reference strain sequences from different parts of the world that are accessible in the NCBI GenBank database. The sequences of the adenovirus strains indicated that they belonged to five species, Fowl aviadenovirus A-E, represented by eight serotypes FAdV-1, FAdV-4, FAdV-5, FAdV-7, FAdV-8a, FAdV-8b, and FAdV-2/11 (FAdV-D). The relationships between FAdVs isolated in Poland and isolates from other regions of the world were determined.

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Section: Discussionsupporting

confidence: 54%

Phylogenetic and geographic analysis of fowl adenovirus field strains isolated from poultry in Poland

Niczyporuk

2015

Arch Virol

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“…Due to that , this region is very useful in the studies on phylogenetic properties and genotyping of adenoviruses (15). The present study was focused on the region of hexon gene as a target for the PCR primers.…”

Section: Discussionmentioning

confidence: 99%

Analysis of adenovirus strains isolated from poultry in Poland

Niczyporuk

Samorek-Salamonowicz

Czekaj

2013

Bulletin of the Veterinary Institute in Pulawy

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The study describes successful isolation of 96 fowl adenovirus (FAdV) strains from 789 chickens from 95 flocks. PCR specific for hexon gene encoding L1 loop was conducted. Amplicons were subjected to sequence analysis. The sequences were analysed by the software: BLAST, Geneious 6.0, and MEGA 5, then aligned with different adenovirus strain reference sequences accessible in GenBank database. The examined strains belong to the particular groups and serotypes. The sequences of all adenoviruses were classified into five species (FAdV A-E) and eight serotypes .

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“…The Japanese strain TR22 272 (presently FAdV-5) was recognized as a member of a separate species (AIC weight <0.1). Partly 273 consistent with the species subdivision proposed by Marek et al, 2010(Marek et al 2010, the 274 species identified here were named by adding a number to the presently used species letter (e.g. 275…”

Section: Species Delineation Analysis 187mentioning

confidence: 99%