Classification of lignocellulosic matrix of spines in Cactaceae by Py-GC/MS combined with omic tools and multivariate analysis: A chemotaxonomic approach

Reyes-Rivera, Jorge; Solano, Eloy; Terrazas, Teresa; Soto-Hernández, Marcos; Arias, Salvador; Almanza-Arjona, Yara C.; Polindara‐García, Luis A.

doi:10.1016/j.jaap.2020.104796

Cited by 7 publications

(30 citation statements)

References 35 publications

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“…Tools derived from the "omics" field, such as multivariate analysis or other cheminformatic data processing techniques, can prove useful in deconvoluting this information and recognizing the unique "fingerprint" of various components in complex mixtures such as lignin. One recent study used Py-GC-MS in tandem with multivariate statistical methods to classify 15 species of Cactaceae based on the composition of the lignocellulosic matrix in their spines (Reyes-Rivera et al, 2020). After preparing and milling the spine samples, the researchers performed Py-GC-MS experiments according to their previously published method (Reyes-Rivera et al, 2018).…”

Section: Modifications and Improvementsmentioning

confidence: 99%

Mass spectrometry‐based methods for the advanced characterization and structural analysis of lignin: A review

Letourneau

Volmer

2021

Mass Spectrometry Reviews

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Lignin is currently one of the most promising biologically derived resources, due to its abundance and application in biofuels, materials and conversion to value aromatic chemicals. The need to better characterize and understand this complex biopolymer has led to the development of many different analytical approaches, several of which involve mass spectrometry and subsequent data analysis. This review surveys the most important analytical methods for lignin involving mass spectrometry, first looking at methods involving gas chromatography, liquid chromatography and then continuing with more contemporary methods such as matrix assisted laser desorption ionization and time-of-flight-secondary ion mass spectrometry. Following that will be techniques that directly ionize lignin mixtures-without chromatographic separation-using softer atmospheric ionization techniques that leave the lignin oligomers intact. Finally, ultra-high resolution mass analyzers such as FT-ICR have enabled lignin analysis without major sample preparation and chromatography steps. Concurrent with an increase in the resolution of mass spectrometers, there have been a wealth of complementary data analyses and visualization methods that have allowed researchers to probe deeper into the "lignome" than ever before. These approaches extract trends such as compound series and even important analytical information about lignin substructures without performing lignin degradation either chemically or during MS analysis. These innovative methods are paving the way for a more comprehensiveThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Modifications and Improvementsmentioning

confidence: 99%

Mass spectrometry‐based methods for the advanced characterization and structural analysis of lignin: A review

Letourneau

Volmer

2021

Mass Spectrometry Reviews

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“…On the other hand, Reyes-Rivera et al [72] applied multivariate analysis for the results obtained in Py-GC/ MS studies of spines in Cactaceae. Principal component analysis (PCA), hierarchical clustering analysis (HCA) and hierarchical clustering on the principal components with k-means partition (HCPC) were applied in this case.…”

Section: Application Of Multivariate Analysismentioning

confidence: 99%

Chromatographic analysis of bio-oil formed in fast pyrolysis of lignocellulosic biomass

Grams

2020

Reviews in Analytical Chemistry

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Fast pyrolysis of lignocellulosic biomass is one of the most promising methods of the production of renewable fuels. However, an optimization of the conditions of bio-oil production is not possible without comprehensive analysis of the composition of formed products. There are several methods for the determination of distribution of products formed during thermal decomposition of biomass with chromatography being the most versatile among them. Although, due to the complex structure of bio-oil (presence of hundreds chemical compounds with different chemical character), an interpretation of the obtained chromatograms is not an easy task. Therefore, the aim of this work is to present an application of different chromatographic methods to the analysis of the composition of the mixture of products formed in high temperature decomposition of lignocellulosic feedstock. It includes pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), two dimensional gas (GC x GC) or liquid chromatography (LC x LC) and initial fractionation of bio-oil components. Moreover, the problems connected with the analysis of bio-oils formed with the use of various fast pyrolysis reactors and capabilities of multivariate analysis are discussed.

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“…Recently, plant spines have been reported to be particularly enriched with phenylpropanoidderived compounds, specifically from lignocellulosic matrix [14,34,35]. In this work, an ultra-performance liquid chromatography coupled to mass spectrometry has been developed for the analysis of secondary metabolites in A. fourcroydes spines, which could be involved in aposematic coloration, a feature typically encountered in agave spines [4,8].…”

Section: Metabolomic Profiling and In Situ Secondary Polyphenols Detementioning

confidence: 99%

“…Apical spines can be brown reddish, gray, black, white, or yellow, rigid or flexible, straight or curved, and prominently long in some species, especially in those belonging Salmianae section [4,[8][9][10]. In contrast to Cactaceae spines, for which chemical composition and ultra-structural characteristics have been described [11][12][13][14], structural composition and anatomy of agave spines remain uncovered. All described agave species possess highly colored spines that suggest an interesting regulatory interplay between metabolite accumulation and sclerenchyma development, and they may become an outstanding model to design fibers that produce its own color.…”

Section: Introductionmentioning

confidence: 99%

“…In the Asparales order, which Agavaceae belong to, saponin glycosides, mostly from roots, have showed to be good markers for chemotaxonomic classifications of Asparagus genotypes [20][21][22]. In xerophyte species of Cactaceae, omics tools have revealed a good taxonomic correspondence between lignin derivatives from spines and the species [14]. Here, hyphenated chromatographic and histochemistry approaches were used to characterize spines chemical composition of Agave fourcroydes Lem., a species traditionally used for fiber extraction, and more particularly their structural carbohydrates, lignin monolignol subunits profiling, and metabolite landscape of terminal spines.…”

Section: Introductionmentioning

confidence: 99%

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Unravelling Chemical Composition of Agave Spines: News from Agave fourcroydes Lem.

Morán-Velázquez

Monribot‐Villanueva

Bourdon

et al. 2020

Plants

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Spines are key plant modifications developed to deal against herbivores; however, its physical structure and chemical composition have been little explored in plant species. Here, we took advantage of high-throughput chromatography to characterize chemical composition of Agave fourcroydes Lem. spines, a species traditionally used for fiber extraction. Analyses of structural carbohydrate showed that spines have lower cellulose content than leaf fibers (52 and 72%, respectively) but contain more than 2-fold the hemicellulose and 1.5-fold pectin. Xylose and galacturonic acid were enriched in spines compared to fibers. The total lignin content in spines was 1.5-fold higher than those found in fibers, with elevated levels of syringyl (S) and guaiacyl (G) subunits but similar S/G ratios within tissues. Metabolomic profiling based on accurate mass spectrometry revealed the presence of phenolic compounds including quercetin, kaempferol, (+)-catechin, and (−)-epicatechin in A. fourcroydes spines, which were also detected in situ in spines tissues and could be implicated in the color of these plants’ structures. Abundance of (+)-catechins could also explain proanthocyanidins found in spines. Agave spines may become a plant model to obtain more insights about cellulose and lignin interactions and condensed tannin deposition, which is valuable knowledge for the bioenergy industry and development of naturally dyed fibers, respectively.

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Classification of lignocellulosic matrix of spines in Cactaceae by Py-GC/MS combined with omic tools and multivariate analysis: A chemotaxonomic approach

Cited by 7 publications

References 35 publications

Mass spectrometry‐based methods for the advanced characterization and structural analysis of lignin: A review

Mass spectrometry‐based methods for the advanced characterization and structural analysis of lignin: A review

Chromatographic analysis of bio-oil formed in fast pyrolysis of lignocellulosic biomass

Unravelling Chemical Composition of Agave Spines: News from Agave fourcroydes Lem.

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