Classification of <i>Stevia rebaudiana</i> Using Near Infrared Spectroscopy and Multivariate Data Analysis

Abstract

Stevia rebaudiana leaf contains stevioside and rebaudioside A as main diterpene glycosides. These compounds are used as natural sweetener and potentially as drug candidate of diabetes type 2. Rapid and nondestructive method for S. rebaudiana leaves (n = 23) classification based on geographical area and main diterpene glycosides content was carried out using near infrared spectroscopy combined with multivariate data analysis. Linear discriminant analysis (LDA) was applied to discriminate S. rebaudiana leaves based on geographical area. Principal component analysis (PCA) was established to classify S. rebaudiana leaves based on main diterpene glycosides content. HPLC analysis was used as reference data to divide PCA result into groups. LDA model correctly classified 95% of the S. rebaudiana leaves based on geographical area. PCA model correctly classified 95% and 90% of S. rebaudiana leaves based on rebaudioside A and stevioside content, respectively. The classification method using near infrared spectroscopy combined with multivariate data analysis demonstrate potential use of the classification method established as quality control technique of S. rebaudiana leaves.

Bioinspired Stevia rebaudiana Green Zinc Oxide Nanoparticles for the Adsorptive Removal of Antibiotics from Water

“…The FTIR spectrum of the S. rebaudiana extract ( Figure 1 b) displays a variety of functional groups, starting with a wide band at 3500–3400 cm –1 , which corresponds to the OH stretch, followed by the sp 3 C–H stretch at 3000–2800 cm –1 . 49 , 66 , 67 The spectrum also shows bands assigned to the C=O stretch of carboxylic acids at 1800–1600 cm –1 , 55 , 59 , 66 a C=C alkene stretch at 1500–1300 cm –1 , 55 , 63 and an ester sulfate band at 1260–1257 cm –1 . 68 The band at 1200–1050 cm –1 could correspond to the C–O stretch of alcohols, ethers, or esters and could be indicative of a glycosidic bond and/or acidic polysaccharides.…”

“…The FTIR spectrum of the S. rebaudiana extract (Figure b) displays a variety of functional groups, starting with a wide band at 3500–3400 cm –1 , which corresponds to the OH stretch, followed by the sp 3 C–H stretch at 3000–2800 cm –1 . ,, The spectrum also shows bands assigned to the CO stretch of carboxylic acids at 1800–1600 cm –1 , ,, a CC alkene stretch at 1500–1300 cm –1 , , and an ester sulfate band at 1260–1257 cm –1 . The band at 1200–1050 cm –1 could correspond to the C–O stretch of alcohols, ethers, or esters and could be indicative of a glycosidic bond and/or acidic polysaccharides. ,,, The band at 900–800 cm –1 corresponds to the C–H vibration of alkenes and alkynes. , Last, the band at 600–400 cm –1 could be attributed to O–H vibrations in phenols and alcohols or an alkyl halide bond. , The FTIR spectrum of the green ZnO NPs synthesized using the S.…”

“… 68 The band at 1200–1050 cm –1 could correspond to the C–O stretch of alcohols, ethers, or esters and could be indicative of a glycosidic bond and/or acidic polysaccharides. 59 , 66 , 68 , 69 The band at 900–800 cm –1 corresponds to the C–H vibration of alkenes and alkynes. 55 , 66 Last, the band at 600–400 cm –1 could be attributed to O–H vibrations in phenols and alcohols or an alkyl halide bond.…”

“…59 , 66 , 68 , 69 The band at 900–800 cm –1 corresponds to the C–H vibration of alkenes and alkynes. 55 , 66 Last, the band at 600–400 cm –1 could be attributed to O–H vibrations in phenols and alcohols or an alkyl halide bond. 55 , 70 The FTIR spectrum of the green ZnO NPs synthesized using the S. rebaudiana extract ( Figure 1 b) is also displayed.…”