Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF)

Ehrenfest, David M. Dohan; Rasmusson, Lars; Albrektsson, Tomas

doi:10.1016/j.tibtech.2008.11.009

Cited by 1,532 publications

(1,399 citation statements)

References 56 publications

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“…Platelet-rich growth factor (PRGF) has been proposed as an aid in enhancing hard and soft tissue regeneration in the field of oral surgery 14) as it is a simple, natural, and inexpensive tool for production of leucocyte and platelet concentrates [21][22][23] . It is a consistent fibrin biomaterial and not improved fibrin glue from the platelet rich plasma family 21) .…”

Section: Introductionmentioning

confidence: 99%

Effect of PRF and Allograft Use on Immediate Implants at Extraction Sockets with Periapical Infection —Clinical and Cone Beam CT Findings—

Medikeri

Meharwade

Wate

et al. 2018

Bull. Tokyo Dent. Coll.

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Immediate implant placement can be successful, even at infected sites. The adjunctive effects of concomitant use of platelet-rich fibrin (PRF) and decalcified freeze-dried bone allografts (DFDBA) at periapically infected sites remains to be determined, however. The purpose of this prospective study was to investigate the effect of combined use of PRF and DFDBA on immediate implant survival at tooth extraction sites exhibiting periapical lesions. Implants were immediately placed in 8 patients under a standard chemotherapeutic protocol. Adin titanium implants were used in all cases. The combination of PRF and DFDBA was used to fill the gap between the implant body and the surrounding socket wall. The final restoration was placed after 3 months. The full-mouth plaque, gingival bleeding index, and gingival esthetics scores were assessed at 3, 6, and 12 months. Cone beam computed tomography images obtained at baseline and at 12 months after implant loading were analyzed. The plaque index scores showed statistically significant differences at 3, 6, and 12 months (p<0.05). The gingival bleeding index score showed no significant difference. No difference was noted in buccal gingival level on the implant surface or adjacent teeth at 91.7% of sites. Complete closure of the interproximal space was seen in 91.7% of the implant sites. Crestal bone levels on all implant surfaces were non-significant. Implant survival was 91.67% at 12 months. The adjunctive use of PRF with DFDBA at periapically infected sites yielded a significant reduction in bone resorption and accelerated bone healing during the initial post-extraction stage. A significant improvement was achieved in the gingival esthetic score at the interproximal and midfacial surfaces. The combined use of growth factors with pre-and postoperative broadspectrum antibiotics over a short time resulted in a higher implant survival rate at the end of the 1 year post-restoration period.

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Section: Introductionmentioning

confidence: 99%

Effect of PRF and Allograft Use on Immediate Implants at Extraction Sockets with Periapical Infection —Clinical and Cone Beam CT Findings—

Medikeri

Meharwade

Wate

et al. 2018

Bull. Tokyo Dent. Coll.

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“…Also, it is easy, simple and low cost that allows to obtain PRF concentrates quickly and by natural means. Hence it can be said that in daily clinical practice it is a most suitable method [17] Platelet rich fibrin and platelet rich plasma differs in preparation technique and other aspect. For PRP production is a twostep centrifugation, anticoagulant is added for blood collection, and biochemical's like calcium chloride bovine thrombin is added for artificial polymerization of platelet concentrate, but for PRF is one step centrifugation, polymerizing naturally and slowly, does not require any addition of biochemical or elaborate procedure.…”

Section: International Journal Of Dental Sciences and Researchmentioning

confidence: 99%

“…Dental pulp cells have also shown to maintain its original morphology and were observed to be attached at the edge of PRF under phase contrast microscopy. [21,22,23] Vital pulpotomy is an universally accepted therapy for incompletely form roots [24].…”

Section: International Journal Of Dental Sciences and Researchmentioning

confidence: 99%

Tissue Engineering: An Analogous Platelet Rich Fibrin for Coronal Pulpotomy in Revival of Pulp Tissue Vitality in Adult Molar with Pulpitis, a Case Report

Aleid¹

2018

IJDSR

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Aim: management of a clinical case with irreversible pulpitis for young adult molar with closed apices using analogous blood derivative PRF and evaluating the clinical and radiographic success for coronal pulpotomy. Summary: A 18 years old male patient reported to the screening clinic, was referred to endodontic clinic with acute pulpitis diagnosed in left first molar tooth. Deep occlusal caries involving pulp, with history of spontaneous lingering pain associated with sign and symptoms of pulpitis. Standard clinical care protocol was strictly implemented, rubber dam isolation was followed by coronal pulpotomy. Blood drawn from cuboidal vein. Platelet rich fibrin membrane placed over the remaining pulp and restored with MTA and finally with Glass ionomer cement. Follow-up results clinically and radio-graphically were encouraging and successful at day 1 to 24 months. No postoperative pain, the treated tooth responded to pulp sensibility test and radiographs revealed periapical healing. However, for standardization of the clinical procedure well designed research study is further needed.

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“…For the concentration step, the supernatant composed of platelets and plasma is collected and centrifuged again to isolate the platelets. 238,239 However, in the literature, several isolation procedures can be found, and they can be divided in three main groups according to the differing parameters in the platelet isolation: (1) final concentration of platelets in PRP; (2) protocol for the activation of PRP; (3) other variations from the standard protocol. 240 There are several classifications to categorize platelet concentrates based on relative concentrations of platelets, leukocytes, and fibrin, but herein the general abbreviation is going to be used.…”

Section: From Single To Multiple Bioactive Factor Delivery For Skeletmentioning

confidence: 99%

“…257 Platelets store essential GF, including PDGF, TGF-b, IGF-1, and EGF. 238,239,253,284 PRP may act as an exogenous source of TGF-b for bone healing, directing BMSCs to resorption sites. 285 Furthermore, platelets contain different angiogenic factors, such as VEGF, Ang-2, FGF-2, and antiangiogenic proteins, including endostatin and thrombospondin-1, regulating the formation of new blood vessels.…”

Section: (2011)mentioning

confidence: 99%

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

Santo

Gomes²,

Mano³

et al. 2013

Tissue Engineering Part B: Reviews

105

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The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of bioactive factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth factors and cytokines involved in each biological response, it is expected that an appropriate combination of bioactive factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple bioactive factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of bioactive factors. A comprehensive collection of studies focused on the delivery of bioactive factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth factors.

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Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF)

Cited by 1,532 publications

References 56 publications

Effect of PRF and Allograft Use on Immediate Implants at Extraction Sockets with Periapical Infection —Clinical and Cone Beam CT Findings—

Effect of PRF and Allograft Use on Immediate Implants at Extraction Sockets with Periapical Infection —Clinical and Cone Beam CT Findings—

Tissue Engineering: An Analogous Platelet Rich Fibrin for Coronal Pulpotomy in Revival of Pulp Tissue Vitality in Adult Molar with Pulpitis, a Case Report

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

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