Classifying<i>Drosophila</i>Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing

Li, Hongjie; Horns, Felix; Wu, Bing; Xie, Qijing; Li, Jiefu; Li, Tongchao; Luginbuhl, David J.; Quake, Stephen R.; Luo, Liqun

doi:10.1101/145045

Cited by 73 publications

(140 citation statements)

References 73 publications

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“…Relative levels of Imp and pros in our predicted 614 hemilineage 15B, can therefore be used to infer muscle-specific innervation along the leg. Notch off hemilineages in the VNC (Li et al, 2017). Therefore, the TF code specifying FAN 638 identity may change across development and also differ depending on the regional 639 context.…”

Section: Astrocytes and Ensheathing Glia 541mentioning

confidence: 99%

A single-cell transcriptomic atlas of the adultDrosophilaventral nerve cord

Allen

Neville

Birtles

et al. 2019

Preprint

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The Drosophila ventral nerve cord (VNC) receives and processes descending signals from the brain to produce a variety of coordinated locomotor outputs. It also integrates sensory information from the periphery and sends ascending signals to the brain. We used single-cell transcriptomics to generate an unbiased classification of cellular diversity in the VNC of five-day old adult flies. We produced an atlas of 26,000 high-quality cells, representing more than 100 transcriptionally distinct cell types. The predominant gene signatures defining neuronal cell types reflect shared developmental histories based on the neuroblast from which cells were derived, as well as their birth order. Cells could also be assigned to specific neuromeres using adult Hox gene expression. This single-cell transcriptional atlas of the adult fly VNC will be a valuable resource for future studies of neurodevelopment and behavior.

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Section: Astrocytes and Ensheathing Glia 541mentioning

confidence: 99%

A single-cell transcriptomic atlas of the adultDrosophilaventral nerve cord

Allen

Neville

Birtles

et al. 2019

Preprint

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“…Over the last two decades, the mechanisms of neuronal wiring specificity in the glomerular map have been intensively studied in Drosophila and mice. In the Drosophila olfactory system, the connectivity of OSN axons and second-order neurons is mostly geneticallydetermined with a variety of transcription factors and cell-surface molecules 16,17 . Notably, the wiring specificity in the Drosophila olfactory system is based on molecular matching between pre-and post-synaptic neurons 18,19 .…”

Section: Introductionmentioning

confidence: 99%

Activity-dependent lateral inhibition signals for synaptic competition

Fujimoto¹,

Leiwe²,

Sakaguchi

et al. 2019

Preprint

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In the mouse olfactory bulb, sensory information detected by ~1,000 types of olfactory sensory neurons (OSNs) is represented by the glomerular map. The second-order neurons, mitral and tufted cells, connect a single primary dendrite to one glomerulus. This forms discrete connectivity between the ~1,000 types of input and output neurons. It has remained unknown how this discrete dendrite wiring is established during development. We found that genetically silencing neuronal activity in mitral cells, but not from OSNs, perturbs the dendrite pruning of mitral cells. In vivo calcium imaging of awake neonatal animals revealed two types of spontaneous neuronal activity in mitral/tufted cells, but not in OSNs. Pharmacological and knockout experiments revealed a role for glutamate and NMDARs. The genetic blockade of neurotransmission among mitral/tufted cells reduced spontaneous activity and perturbed dendrite wiring. Thus, spontaneous network activity generated within the olfactory bulb self-organizes the parallel discrete connections in the mouse olfactory system.

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“…This issue is beginning to be addressed in a variety of neural systems. Applying single-cell transcriptomic analysis to systems with a pre-existing ground truth of cell types and function (e.g., mouse retina: Shekhar et al, 2016; fly olfactory system: Li et al, 2017) has resulted in relatively faithful mapping between molecular and functional types. Interrogation of two subcortically-projecting transcriptomic types in mouse anterolateral motor cortex (ALM) during a motor planning task revealed differences in the encoding of preparatory activity and motor commands (Economo et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Differential encoding in prefrontal cortex projection neuron classes across cognitive tasks

Lui

Nguyen

Grutzner

et al. 2020

Preprint

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Single-cell transcriptomics has been widely applied to classify neurons in the mammalian brain, while systems neuroscience has historically analyzed the encoding properties of cortical neurons without considering cell types. Here we examine how specific transcriptomic types of mouse prefrontal cortex (PFC) projection neurons relate to axonal projections and encoding properties across multiple cognitive tasks. We found that most types projected to multiple targets, and most targets received projections from multiple types, except PFC→PAG (periaqueductal gray). By comparing Ca 2+ -activity of the molecularly homogeneous PFC→PAG type against two heterogeneous classes in several two-alternative choice tasks in freely-moving mice, we found that all task-related signals assayed were qualitatively present in all examined classes. However, PAG-projecting neurons most potently encoded choice in cued tasks, whereas contralateral PFC-projecting neurons most potently encoded reward context in an uncued task. Thus, task signals are organized redundantly, but with clear quantitative biases across cells of specific molecular-anatomical characteristics.

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ClassifyingDrosophilaOlfactory Projection Neuron Subtypes by Single-cell RNA Sequencing

Cited by 73 publications

References 73 publications

A single-cell transcriptomic atlas of the adultDrosophilaventral nerve cord

A single-cell transcriptomic atlas of the adultDrosophilaventral nerve cord

Activity-dependent lateral inhibition signals for synaptic competition

Differential encoding in prefrontal cortex projection neuron classes across cognitive tasks

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