Classmode: A new data format for real‐time multiparameter data analysis and data compression

Abstract

A new data acquisition and analysis format (classmode) was developed that allows real-time data classification in a flow cytometer. In our cytometer, detected events were classified in real time by their presence or absence in a set of look-up tables (LUT). A modification of the cytometer hardware allows the exclusive transfer of the LUT data to the acquisitiodstorage computer. Using a combination of 8 LUTs, the analyzed events can be classified into 256 subpopulations. Real-time data classiflcation results in an increased data transfer rate and a significant compression of the data. Q 1995 WiIey-Liss, Inc.