Clastogenic effects of methoxy-7-nitro-2-naphtho (2,1-b) furan (R7000) on Nigella damascena L.

Evaluation of Chromosome Damage due to a Nitronaphtofuran Derivative: R7372 on the Plant Nigella damascena L.

“…There is also, after treatments of each mitotic stage by one of the two nitronaphtofurans, a drastic uncoiling of chromosomes, sometimes preferentially localised at centromeric regions, eventually leading to centromeric breaks. This uncoiling could be in some ways interpreted as a modification of the action of topo-isomerase (Gilot-Delhalle et al 1994). The synergistic effect with colchicine for metaphases accumulation could be interpreted as an effect localised at two different sites.…”

“…Some mechanisms of action have been proposed for R7000, but it is not known till what extend they can be totally extrapolated to R7372. Electronic disequilibrium would explain various modifications of the nucleophilic character of the the molecules which lead to mutagenic derivatives such as anionic radicals, superoxide anion diverse amines and hydroxylamine (Gilot-delhalle et al 1994, Biaglow et al 1977, Mc Calla 1983.…”

“…The chromosome breaking efficiency of the compound R7000 was previously investigated in the plant Nigella damascena in both seeds and root tips (Gilot-Delhalle et al 1994).…”

“…However, molecules of this class, with enhanced specific mutagenic and carcinogenic properties could be useful for experimental purposes (Gilot-Delhalle et al 1994). The chromosome breaking efficiency of the compound R7000 was previously investigated in the plant Nigella damascena in both seeds and root tips (Gilot-Delhalle et al 1994).…”