Clastogenicity of Beta-Caryophyllene in Mouse

Molina-Jasso, Dolores; Álvarez-González, Isela; Madrigal-Bujaidar, Eduardo

doi:10.1248/bpb.32.520

Cited by 38 publications

(27 citation statements)

References 11 publications

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“…Before the chemical administration we obtained blood from the tail of each mouse, extended it on clean slides, fixed the cells in methanol for 5 min, and stained them with a 4% Giemsa solution made in PBS at a pH 6.8. 10) Then, mice were treated as follows: the control group was intragastrically (i.g.) administered water (0.3 mL per mouse), another group was intraperitoneally (i.p.)…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Duloxetine as Micronuclei Inducer in an Acute and a Subchronic Assay in Mouse

Madrigal-Bujaidar

Álvarez-González

Morales-González

2015

Biological & Pharmaceutical Bulletin

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Duloxetine is a widely used antidepressant worldwide. In the present report, we evaluated its capacity to induce micronucleated polychromatic erythrocytes (MNPEs) and micronucleated normochromatic erythrocytes (MNNEs) in mice. Two assays were performed, one with a single chemical administration and the other with daily chemical administration. In the first, we administered the antidepressant once to groups of 5 mice by the intragastric (i.g.) route (2, 20, and 200 mg/kg) and performed the analysis at 24, 48, and 72 h postadministration. A control group administered i.g. distilled water was included in the assay, as well as another treated with the micronuclei-inducing chemical daunorubicin (2.5 mg/kg, injected intraperitoneally (i.p.)). In this assay, we found significant damage induced by duloxetine starting from the first time evaluated, showing the highest MNPE increase at the end of the assay. We observed a saturation effect as well, suggested by a decreasing relative efficiency with respect to each tested dose. In a second assay, we administered the antidepressant i.g. every day for 5 weeks (2, 6, and 12 mg/kg), and micronuclei analysis was performed at the end of each week. In this study, we also found a significant increase in both MNPEs and MNNEs which was clear by the second week of administration. Our results suggest that short-term as well as cumulative damage is produced by duloxetine. Thus, confirmation of the observed genotoxic potential in other models seems advisable, as well as caution when prescribing this antidepressant.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Duloxetine as Micronuclei Inducer in an Acute and a Subchronic Assay in Mouse

Madrigal-Bujaidar

Álvarez-González

Morales-González

2015

Biological & Pharmaceutical Bulletin

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“…Based on metabolism of sesquiterpenes, hydroperoxides are the product of caryophyllene metabolism, which are readily converted to caryophyllene oxide, a second compound that is little reactive and more stable (Sköld et al, 2006). This metabolic process, associated with the DNA repair system and detoxification in mice, could explain the absence of genotoxic effects in vivo studies by Molina-Jasso et al (2009) and the divergence with the results found in this study, where tests were performed in vitro.…”

Section: Discussionmentioning

confidence: 57%

“…However, according to Molina-Jasso et al (2009), caryophyllene is considered safe for use in industries and therapeutic purposes. Based on metabolism of sesquiterpenes, hydroperoxides are the product of caryophyllene metabolism, which are readily converted to caryophyllene oxide, a second compound that is little reactive and more stable (Sköld et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

The impact of compounds isolated from Ocimum sp. on apoptotic activity of osteoclast

Juíz¹,

Gambari²,

Piva³

et al. 2016

J. Med. Plants Res.

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Periodontal disease has multifactorial etiology. The immune response to the microbial challenge leads to osteoclast activation and resorption of the alveolar bone, resulting in tooth loss. Compounds isolated from Ocimum americanum and Ocimum basilicum were analyzed for apoptotic activity towards osteoclast in this study. Steam distillation was used for the extraction of essential oils (EOs) from dry leaves and flowers. The assessment of apoptosis in osteoclasts was carried out through the TUNEL assay and immunocytochemistry for the Fas receptor. The chemical profile of EOs, characterized through gas chromatography-mass spectrometry analysis, revealed methyl cinnamate (O. americanum), linalool, caryophyllene, 1,8-cineole (O. basilicum) as major components. The results showed that essential oils were not able to induce apoptosis in osteoclast; however, linalool (50 to 300 μg.ml -1 ) induced 75% of apoptotic osteoclasts at non-toxic concentrations and the apoptotic activity was confirmed by the increasing levels of Fas receptor on osteoclasts treated with this compound. This study suggests that linalool could be used to control osteoclast activity.

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“…The selected doses of BC were based on a previous report that used similar experimental conditions (Molina-Jasso et al, 2009). Three groups of mice were treated with 20, 200, and 2,000 mg/kg of BC each; a control group was administered with corn oil (10% w/v); another group was injected with 200 mg/kg of BaP; and finally, three other groups were treated with 20, 200, and 2,000 mg/kg of BC, respectively, and 30 min later they were ip injected with 200 mg/kg of BaP.…”

Section: Genotoxicity/antigenotoxicity: Sce and Ca Determinationsmentioning

confidence: 99%

“…Regarding this chemical, various biomedi-cal effects has been reported such as anti-inflammatory, antibacterial, hepatoprotector, and neuroprotector (Calleja et al, 2013;Chang et al, 2013); moreover, its action as antioxidant and glutation S transferase (GST) inducer has also been determined (Calleja et al, 2013;Zheng et al, 1992). In the genotoxicity area, authors have found no damaging effect by using the Salmonella revertant test, as well as by the analysis of micronuclei in cultured human lymphocytes and in mice in vivo (Di Sotto et al, 2008Molina-Jasso et al, 2009). In the case of its antimutagenic capacity, BC has been studied only in in vitro assays with results showing, a reduction of the DNA damage, as well as no effect (Di Sotto et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Antigenotoxic capacity of beta-caryophyllene in mouse, and evaluation of its antioxidant and GST induction activities

2014

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-The present report was designed to determine the antigenotoxic capacity of beta-caryophyllene (BC) on the damage induced by benzo(a)pyrene (BaP) in mouse. We found no genotoxic potential of BC, and a significant inhibitory effect on the number of sister-chromatid exchanges (SCE) and chromosomal aberrations induced by BaP. The three tested doses of the agent (20, 200, and 2,000 mg/kg) produced a dose-dependent decrease of the two evaluated cytogenetic parameters. In comparison with the effect induced by BaP, the best inhibitory effect (about 80%) was obtained with the high tested dose of BC considering the two evaluated parameters. Other aim of the study was to explore whether in this effect participated the BC antioxidant capacity and/or its effect as inducer of GST activity. We found a dose-dependent decrease induced by BC in regard to both the oxidation of lipids and proteins produced by BaP. In the case of GST, when BC was administered alone we found a mean increase of 64% of the enzyme activity, respect to the control level, and when BC was administered in mice treated with BaP the increase obtained with the high dose of BC reached 27%. Therefore, our data established no in vivo genotoxicity by BC, and a significant antigenotoxic potential of the compound, which may be related with its capacity to block the molecular oxidation and to stimulate the GST activity.

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Clastogenicity of Beta-Caryophyllene in Mouse

Cited by 38 publications

References 11 publications

Evaluation of Duloxetine as Micronuclei Inducer in an Acute and a Subchronic Assay in Mouse

Evaluation of Duloxetine as Micronuclei Inducer in an Acute and a Subchronic Assay in Mouse

The impact of compounds isolated from Ocimum sp. on apoptotic activity of osteoclast

Antigenotoxic capacity of beta-caryophyllene in mouse, and evaluation of its antioxidant and GST induction activities

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