Claudin expression in pancreatic endocrine tumors as compared with ductal adenocarcinomas

Borka, Katalin; Kaliszky, Péter; Szabó, Erzsébet; Lotz, Gábor; Kempler, Péter; Schaff, Zsuzsa; Kiss, András

doi:10.1007/s00428-007-0406-7

Cited by 46 publications

(48 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Claudin expression alterations in primary cancers are much better characterized, including those of the breast (Kominsky et al 2003;Tokes et al 2005), colon (de Oliveira et al 2005;Dhawan et al 2005), prostate (Long et al 2001), and pancreas (Nichols et al 2004;Borka et al 2007). Our group has also previously defined tight junctional protein changes in primary and metastatic cancers of the liver.…”

mentioning

confidence: 99%

Distinct Claudin Expression Profiles of Hepatocellular Carcinoma and Metastatic Colorectal and Pancreatic Carcinomas

Holczbauer

Gyöngyösi

Lotz

et al. 2013

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

Tight junction proteins, including claudins, are often dysregulated during carcinogenesis and tumor progression. Moreover, the claudin expression pattern usually varies between different tumor entities. We aimed to investigate claudin expression profiles of primary and metastatic liver malignancies. We analyzed claudin-1, -2, -3, -4, and -7 expression by quantitative immunohistochemistry and real-time RT-PCR, respectively. Twenty hepatocellular carcinomas (HCCs) and liver metastases of 20 colorectal adenocarcinomas (CRLMs) and 15 pancreatic adenocarcinomas (PLMs) were studied together with paired surrounding non-tumorous liver samples and 5 normal liver samples. Strong claudin-3 and -7 immunohistochemical positivities were detected in CRLM samples, each with significantly stronger staining when compared with HCC and PLM groups. Claudin-1 protein was found highly expressed in CRLM, in contrast to lower expression in PLM and HCC. CRLMs and PLMs also were strongly positive for claudin-4, while being virtually undetectable in HCC. Claudin-2 showed strong positivity in non-tumorous liver tissue, whereas significantly weaker positivity was observed in all tumors. Differences in mRNA expression were mostly similar to those found by immunohistochemistry. In conclusion, HCC and both CRLM and PLM display distinct claudin expression profiles, which might provide better understanding of the pathobiology of these lesions and might be used for differential diagnosis.

show abstract

mentioning

confidence: 99%

Distinct Claudin Expression Profiles of Hepatocellular Carcinoma and Metastatic Colorectal and Pancreatic Carcinomas

Holczbauer

Gyöngyösi

Lotz

et al. 2013

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…These discrepant results may be due to the difference between number and characteristics of the cases of the studies. Borka et al (2007) showed expression of Claudin 1, 4 and 7 in pancreatic adenocarcinoma (Borka et al, 2007). Tsukahara et al (2005) investigated the expression of claudin-4 in human pancreas, pancreatic ductal adenocarcinomas, and intraductal papillarymucinous tumors of the pancreas (IPMT), and compared with that of claudin-1.…”

Section: Discussionmentioning

confidence: 99%

Expression Pattern and Prognostic Significance of Claudin 1, 4 and 7 in Pancreatic Cancer

Ali̇kanoğlu¹,

Gündüz²,

Demirpençe³

et al. 2015

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: Tight junctions (TJs) organise paracellular permeability and they have an important role in epithelial and endothelial cell polarity and permanence of barrier function. It has been demonstrated that the Claudin family constitutes an important component of them. In this study, we assessed expression patterns of of Claudin1, 4 and 7 and whether they have any relation with prognosis in patients with pancreatic cancer. Materials and Methods: Expression patterns of Claudin 1,4 and 7 were examined by immunohistochemistry in 25 patients with a histopathological diagnosis of pancreatic cancer using a semiquantitative scoring of the extent and intensity of staining. After grouping the staining scores as low (final score 0-2) and high (final score 3-9) the relation between expression of Claudin 1,4 and 7 and survival was evaluated. Results: There was no significant relation between expression of Claudin 1,4 and 7 and gender and stage. No statistically significant relation was found between Claudin 1 and 4 expression and survival whereas a statistically significant relation was found between decrease in Claudin 7 expression and decrease in survival. Conclusions: Claudins have important functions other than their popular function known as adhesion. Supporting this hypothesis, we found a statistically significant relationship between increased Claudin 7 expression and increased survival time, and this suggests that Claudin 7 may exert different tumorigenic effects in pancreatic cancer other than its wellknown adhesion effect.

show abstract

“…Claudin-3 and -4 are expressed in many normal organs and are overexpressed in several carcinomas, including colorectal, breast, ovary, prostate and pancreatic carcinomas (4)(5)(6)(7)9), and these proteins are receptors for cytotoxic Clostridium perfringens enterotoxin (CPE) (23,24). CPE is known to injure intestinal epithelial cells expressing claudin-3 and/or -4 by increasing membrane permeability, leading to loss of osmotic equilibrium and subsequent cytolysis and cell death (25).…”

Section: Discussionmentioning

confidence: 99%

“…Immunoreactivity was evaluated as described previously (7,10,13). Ten randomly selected fields in each tissue slide were analyzed using high-power field (x400) with 100 cells counted per field.…”

Section: Evaluation Of Immunoreactivity and In Situ Hybridizationmentioning

confidence: 99%

“…It has been hypothesized that changes and/or loss of claudin expression may play an important role in tumorigenesis and tumor progression, and altered expression of claudins has been reported in a variety of human neoplasms, including colorectal, breast, ovarian, pancreatic, prostate, tongue and esophageal carcinomas and in rectal well-differentiated endocrine neoplasms (4)(5)(6)(7)(8)(9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma

Kojima

Ishida²,

Takikita-Suzuki³

et al. 2010

Oncol Rep

View full text Add to dashboard Cite

Abstract.Claudins are a family of proteins that are structural and functional components of tight junctions and have crucial roles in the maintenance of cellular arrangement, adhesion and paracellular transport. Recent studies have shown that changes and/or loss of claudin expression plays an important role in tumorigenesis and tumor progression, and altered expression of claudins has been reported in various human carcinomas. Non-keratinizing nasopharyngeal carcinoma (NPC) is a common Epstein-Barr virus (EBV)-associated carcinoma with characteristic clinicopathological features. The aim of this study was to investigate claudin expression profiles in EBV-associated non-keratinizing NPC. We analyzed expressions of claudin-1, -2, -3, and -4 in 18 cases of EBV-associated non-keratinizing NPC by immunohistochemical methods. Claudin-1 was expressed in all 18 cases, but claudin-2 was not expressed in any of the 18 cases. Claudin-3 expression was variable, with 8 of the 18 cases (45%) showing no immunoreactivity for claudin-3. Claudin-4 displayed positive immunoreactivity in all cases, even in claudin-3-negative cases. Claudin-3 and -4 are receptors for cytotoxic Clostridium perfringens enterotoxin (CPE) and CPE has emerged as a potential therapeutic target for malignant tumors expressing claudin-3 and/or -4, because CPE specifically and rapidly lyses cells expressing these proteins. Clinically, treatment of distant metastases is a serious problem in EBV-associated non-keratinizing NPC, because frequently there is lymph node involvement and distant metastasis before detection of the primary tumor.Therefore, CPE therapy may be a potential therapeutic target for EBV-associated non-keratinizing NPC, since our results clearly showed claudin-3 and/or -4 expression in all cases studied.

show abstract

Claudin expression in pancreatic endocrine tumors as compared with ductal adenocarcinomas

Cited by 46 publications

References 42 publications

Distinct Claudin Expression Profiles of Hepatocellular Carcinoma and Metastatic Colorectal and Pancreatic Carcinomas

Distinct Claudin Expression Profiles of Hepatocellular Carcinoma and Metastatic Colorectal and Pancreatic Carcinomas

Expression Pattern and Prognostic Significance of Claudin 1, 4 and 7 in Pancreatic Cancer

Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma

Contact Info

Product

Resources

About