Cleaning a crime scene 2.0—what to do with the bloody knife after the crime?

Helmus, Janine; Poetsch, Jeremy; Pfeifer, Manuel; Bajanowski, Thomas; Poetsch, Micaela

doi:10.1007/s00414-019-02162-6

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…A higher resilience of saliva samples has already been demonstrated [ 22 ] and may be due to cell composition (salivary cells, inflammatory cells) or the presence of glycoproteins [ 30 ] which enhance the adhesion of cells and DNA to the surface [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

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About the influence of environmental factors on the persistence of DNA — a long-term study

Poetsch¹,

Markwerth²,

Konrad³

et al. 2022

Int J Legal Med

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DNA persistence and DNA transfer are important features in the assessment of a crime scene. The question how long DNA may persist at a certain location is similarly important as the one how the DNA has been transferred to this location. Depending on the source of the DNA as well as the conditions at the crime scene, the answer to this question is quite difficult. In this study, persistence of DNA from epithelial abrasions, blood cells, and saliva cells in indoor and outdoor scenarios has been investigated with regard to exposure time and exposure conditions including sunlight, temperature, and humidity in summer and winter scenarios. Overall, we generated 338 epithelial samples, 572 blood samples, and 572 saliva samples. A complete profile of the cell/DNA donor after exposure could be obtained in 47%, 65%, and 58% of epithelial abrasions, blood samples, and saliva samples, respectively. Regarding blood samples, there were no differences between supporting materials cloth and plastic; however, the percentage of complete profiles was higher for saliva samples on plastic and for epithelial samples on cloth. In indoor scenarios, complete profiles could be recovered from nearly all blood and saliva samples up to 9 months, whereas the amount of epithelial complete profiles already started to decline after 3 months. In outdoor scenarios, we observed a tipping point at an exposure time of 3 months. Blood and saliva samples collected after this period displayed complete profiles in less than 25% of samples. After 12 months, no outdoor sample showed a complete profile. The results of this study facilitate decisions on the relevance of recovered DNA from crime scenes.

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Section: Resultsmentioning

confidence: 99%

“…Collecting of DNA from plastic surfaces as well as cotton clothes was done as described before [ 22 ]. DNA extraction was performed using DNA IQ Casework Pro Kit and Casework Extraction Kit in the Maxwell 16® instrument according to the manufacturer’s instructions (Promega, Mannheim, Germany), resulting in an extraction volume of 50 µl.…”

Section: Methodsmentioning

confidence: 99%

About the influence of environmental factors on the persistence of DNA — a long-term study

Poetsch¹,

Markwerth²,

Konrad³

et al. 2022

Int J Legal Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other studies looked at the persistence of DNA and biological fluids on various non-porous and porous items after cleaning or washing. Helmus et al [132] investigated whether DNA traces (blood, saliva, epithelial cells) on different objects (knives, plates, glasses, and plastic lids) can persist on the surface despite cleaning by different methods (such as washing by hand or use of a dishwasher). They found that small deposits of biological material often still provided sufficient DNA to generate full profiles after the item they were on was rinsed or washed by hand, while profiles were not able to be generated after cleaning in a dishwasher.…”

Section: Persistencementioning

confidence: 99%

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

Oorschot

Meakin

Kokshoorn

et al. 2021

Genes

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Understanding the factors that may impact the transfer, persistence, prevalence and recovery of DNA (DNA-TPPR), and the availability of data to assign probabilities to DNA quantities and profile types being obtained given particular scenarios and circumstances, is paramount when performing, and giving guidance on, evaluations of DNA findings given activity level propositions (activity level evaluations). In late 2018 and early 2019, three major reviews were published on aspects of DNA-TPPR, with each advocating the need for further research and other actions to support the conduct of DNA-related activity level evaluations. Here, we look at how challenges are being met, primarily by providing a synopsis of DNA-TPPR-related articles published since the conduct of these reviews and briefly exploring some of the actions taken by industry stakeholders towards addressing identified gaps. Much has been carried out in recent years, and efforts continue, to meet the challenges to continually improve the capacity of forensic experts to provide the guidance sought by the judiciary with respect to the transfer of DNA.

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“…Forensic pathology is a term used to describe forensic medicine also known as medical jurisprudence or legal medicine [1][2][3]. In the subject of forensic medicine, medical evidence (samples) are gathered and examined to generate factual data for use in the legal system Forensic medicine entails the application of scientific medical knowledge to the administration of justice, the advancement of justice, and the legal responsibilities of the medical profession [2,3]. It is most prominent sub-field of forensic science that deals with the examination of human body using various components found at the crime scene.…”

Section: Introductionmentioning

confidence: 99%

Blood as an Important Tool in Forensic Medicine in Sub-Saharan Africa: Nigeria as a Perception

Osho¹,

Joy²

2023

J Pathol Res Rev Rep

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Blood is one of the most important biological tools in forensic medicine used to resolve and investigate crime-related cases because it contains useful details. It entails an analysis of various aspects of bloodstains that contribute to the clarification of some violent criminal cases. The knowledge of forensic medicine is essential to provide answers about a series of events and mechanisms of the creation of specific groups of bloodstains on the crime scene. This includes investigation towards the analysis of bloodstains. This knowledge enables the interpretation of results and makes it possible to get closer to the truth, solve particular crimes and ensure accuracy in the legal determination of criminal offences which helps in a proper penalty when making a judgment for the offender

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Cleaning a crime scene 2.0—what to do with the bloody knife after the crime?

Cited by 7 publications

References 24 publications

About the influence of environmental factors on the persistence of DNA — a long-term study

About the influence of environmental factors on the persistence of DNA — a long-term study

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

Blood as an Important Tool in Forensic Medicine in Sub-Saharan Africa: Nigeria as a Perception

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