Clear cell carcinoma of the human ovary synthesizes and secretes a transferrin with microheterogeneity of lectin affinity

Abstract

Human ovarian clear cell carcinoma cell line (transferrin (Tf)-non-producer), HAC 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 pg/ml of insulin at least for 6 months. They synthesized and secreted constantly the 80 kDa protein immunologically similar to human serum Tf (15 f 12 ng/ml/lO' cells/3 days). By sensitive lectin-affinity electrophoresis followed by antibody-affinity blotting technique, a concanavalin A weakly bound or unbound, lentil lectin, a strongly reactive abnormal band, which was rarely found in human serum Tf, was detectable in the Tf synthesized by HAC 2 cells (HACTf). These fmdings suggest that the HACTf may act as one of the autocrine growth factors and that this heterogeneity of HACTf for lectin affinity is ascribed to differences in the carbohydrate moiety of the Tf.

Prognostic Factors in Chronic Lymphocytic Leukemia

“…Reduced iron absorption due to inflammation and/or increased iron utilization by prostate cancer cells is the suggested mechanism o f reduced ferritin and transferrin levels in the serum o f prostate cancer patients. Transferrin synthesis by T lymphocytes (Lum, Infante et al 1986), ovarian cancer cell lines (Ohkawa, Takada et al 1990) and small cell lung cancer have been demonstrated (Vostrejs, Moran et al 1988). Transferrin has been detected in the ascites o f ovarian cancer patients (Ahmed, Oliva et al 2005).…”

Acetaldehyde‐Induced Growth Retardation and Micro‐Heterogeneity of the Sugar Chain in Transferrin Synthesized by HepG2 Cells

“…Transferrin was purified using an immunoaffinity procedure as reported previously (Ohkawa et al, 1990). In brief, polyclonal goat antibody against human transferrin was obtained from Cappel (No.…”

“…Informed consent was obtained for all serum samples. Transferrin concentration was measured by an enzyme-linked immunosorbent assay method as previously reported (Ohkawa et al, 1990).…”