Clear cell variant of squamous cell carcinoma originating in the esophagus: Report of a case with immunohistochemical and oncogenetic analyses

Abstract

The cutaneous clear cell squamous cell carcinoma (SCC) is a rare tumor thought to be associated with hair follicle or skin appendage differentiation. We report herein a rare variant case of a clear cell SCC originating in the esophagus. A 70-year-old Japanese man was found to have a tumor in the esophagus. The excised neoplasm showed dominance of clear cell over conventional SCC components; the two components in an apparent continuum. The clear cells, regular in size with a moderate nuclear/cytoplasmic ratio and relatively hyperchromatic and centrally located nuclei, were compactly arranged in sheets. Glycogen deposition was apparent on PAS staining with or without diastase digestion and under the electron microscope. The clear cell SCC components were positive for cytokeratin (CK)7, CK8, CK18 and CK19, but were negative for CK5/6 or CK14. Reciprocal staining patterns of CKs were apparent in conventional SCC components. The present case and cutaneous clear cell SCC counterparts share some histopathologic characteristics whereas CKs expression differs between the two. Overexpression of p53 protein, without evidence of any mutation, and reduced p16(INK4a) were noted in both clear cell and conventional SCC components. No mutations of Kras, BRAF or β-catenin genes were found in both tumor components.

Protein expression and methylation of DNA repair genes hMLH1, hMSH2, MGMT and BRCA1 and their correlation with clinicopathological parameters and prognosis in basal‐like breast cancer

“…The p53 gene sequence from exons 6–8 was screened in each tumour‐derived DNA sample. Primer sequences and protocols have been described previously . Purified PCR products were sequenced with dideoxynucleotides (BigDye Terminator version 3.1; Applied Biosystems, Foster City, CA, USA) and specific primers, purified using a BigDye X Terminator Purification Kit (Applied Biosystems), and then analysed with a capillary sequencing machine (3730xl Genetic Analyzer; Applied Biosystems).…”

Immunohistochemical and oncogenetic analyses of the esophageal basaloid squamous cell carcinoma in comparison with conventional squamous cell carcinomas

“…Mutations of these genes were examined in exons 4 to 10 of p53, exon 3 of CTNNB1, and exons 18 to 21 of EGFR by polymerase chain reaction followed by direct sequencing. The primer sequences for p53 exons 4 to 10 and CTNNB1 exon 3 in this study were as previously described [15]. The primer sequences for EGFR exons 18 to 21 were as follows: exon 18 forward: 5′ -CAAG-TGCCGTGTCCTGGCACCCAAGC -3′ and reverse: 5′ -CCAAACACTCAGTGAAACAAAGAG -3′; exon 19 forward: 5′ -GCACCATCTCACAATTGCCAGTTA -3′ and reverse: 5′ -AAAAGGTGGGCCTGAGGTTCA -3′; exon 20 forward: 5′ -GAAACTCAAGATCGCATTCATGC -3′ and reverse: 5′ -GCAAACTCTTGCTATCCCAGGAG -3′; exon 21 forward: 5′ -CAGCCATAAGTCCTCGACGTGG -3′ and exon 21 reverse: 5′ -CATCCTCCCCTGCATGTGTTAAAC -3′.…”

Distinct WNT/β-catenin signaling activation in the serrated neoplasia pathway and the adenoma-carcinoma sequence of the colorectum

“…The primer sequences in this study were as previously The WNT/b-catenin and serrated pathway described. 27 Purified PCR products were sequenced with dideoxynucleotides (BigDye Terminator v3.1, Applied Biosystems, Foster City, CA, USA) and specific primers, purified using a BigDye X Terminator Purification Kit (Applied Biosystems), and then analyzed with a capillary sequencing machine (3730xl Genetic Analyzer, Applied Biosystems). Sequences were then examined with Sequencing Analysis V3.5.1 software (Applied Biosystems).…”