Clearance properties of liposomes involving conjugated proteins for targeting

Active targeting of brain tumors using nanocarriers

“…The reaction requires two steps, the first being the crosslinker conjugation and the second, the deprotection of the sulfhydryl groups. Besides, a reaction between 2-iminothiolane hydrochloride known as Traut's reagent and amine groups provided an alternative to SATA and SPDP and required only one step [234]. However, these procedures using primary amines sometimes involved partial or complete loss of recognition activity, due to the modification of the antigen binding sites [235].…”

pH-sensitive immunoliposomes specific to the CD33 cell surface antigen of leukemic cells

“…Different conjugation procedures have been reported for the coupling of targeting ligands to liposomes (Harasym et al, 1998;Nobs et al, 2004). The method chosen is important as it may modify the binding affinity of the antibody to its target.…”

Design of targeted lipid nanocapsules by conjugation of whole antibodies and antibody Fab’ fragments

“…However, this conjugation method can often cause partial or complete loss of recognition activity due to a modification of the antigen binding site. In order to prevent this effect, a thiolation procedure was chosen using the carbohydrate located within the Fc portion [32]. The sugar part of the OX26 MAb was oxidized at 4 1C to convert vicinal hydroxyls into aldehydes.…”