Cleavage of Cholesterol Side Chain by Adrenal Cortex. IV. Effect of Phosphate and Various Nucleotides on a Soluble Enzyme System<sup>*</sup>

Satoh, Paul S.; Constantopoulos, George; Tchen, T.T.

doi:10.1021/bi00869a028

Cited by 21 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phosphate (200 mM) completely abolished the cholesterol side-chain cleavage activity. Satoh et al [21] reported a similar inhibitory effect with phosphate on the adrenal cortex cholesterol side-chain cleavage enzyme. It appears therefore, that ionic strength is important for the activity of the solubilised lipophilic enzyme system, possibly affecting the hydrophobic interactions between the enzyme system and the substrate.…”

mentioning

confidence: 79%

The Cholesterol Side‐Chain Cleavage Enzyme System in Mitochondria of Human Term Placenta

Mason¹,

Boyd²

1971

European Journal of Biochemistry

View full text Add to dashboard Cite

The cholesterol side‐chain cleavage enzyme system of human term placental mitochondria has been studied. Native mitochondria were capable of converting [4‐14C]cholesterol to [4‐14C]‐progesterone in the presence of NADPH and oxygen. Disruption of mitochondrial structure resulted in an inhibition of the enzymic activity. However, active enzyme could be extracted from the disrupted mitochondria by high speed centrifugation, the endogenous inhibitor remaining associated with the particulate material. The nature of the endogenous inhibitor has been determined. It appears to involve the mitochondrial phospholipid, especially lecithin. Investigations with authentic phospholipids confirm these studies. Separation of the enzyme system from the bulk of the mitochondrial material permitted investigation of the characteristics of the enzyme. Light reversibility studies of the carbon monoxide inhibition of the enzymic activity showed the involvement of cytochrome P450 in the enzyme system. Fractionation of the enzyme using gel exclusion and ion—exchange chromatography revealed the involvement of a heam iron protein (presumably cytochrome P450), a component having NADPH‐diaphorase activity (flavoprotein) and a non‐haem iron ‘protein in the enzyme system.

show abstract

mentioning

confidence: 79%

The Cholesterol Side‐Chain Cleavage Enzyme System in Mitochondria of Human Term Placenta

Mason¹,

Boyd²

1971

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…The oxidative cleavage of the cholesterol side-chain has been studied in soluble adrenal-enzy:~e systems; they require oxygen and preferentially NADPH (119), and are stimulated by arsenate or inorganic phosphate; 3',5'adenosine monophosphate (AMP) has no effect. The oxidative cleavage of the cholesterol side-chain has been studied in soluble adrenal-enzy:~e systems; they require oxygen and preferentially NADPH (119), and are stimulated by arsenate or inorganic phosphate; 3',5'adenosine monophosphate (AMP) has no effect.…”

mentioning

confidence: 99%

Biochemistry of Steroids

Sih¹,

Whitlock²

1968

Annu. Rev. Biochem.

View full text Add to dashboard Cite

Mechanism of Action of Androgens

Hilf¹

1975

Antineoplastic and Immunosuppressive Agents

View full text Add to dashboard Cite

Cleavage of Cholesterol Side Chain by Adrenal Cortex. IV. Effect of Phosphate and Various Nucleotides on a Soluble Enzyme System^*

Cited by 21 publications

References 29 publications

The Cholesterol Side‐Chain Cleavage Enzyme System in Mitochondria of Human Term Placenta

The Cholesterol Side‐Chain Cleavage Enzyme System in Mitochondria of Human Term Placenta

Biochemistry of Steroids

Mechanism of Action of Androgens

Contact Info

Product

Resources

About

Cleavage of Cholesterol Side Chain by Adrenal Cortex. IV. Effect of Phosphate and Various Nucleotides on a Soluble Enzyme System*

Cited by 21 publications

References 29 publications

The Cholesterol Side‐Chain Cleavage Enzyme System in Mitochondria of Human Term Placenta

The Cholesterol Side‐Chain Cleavage Enzyme System in Mitochondria of Human Term Placenta

Biochemistry of Steroids

Mechanism of Action of Androgens

Contact Info

Product

Resources

About

Cleavage of Cholesterol Side Chain by Adrenal Cortex. IV. Effect of Phosphate and Various Nucleotides on a Soluble Enzyme System^*