Cleavage pattern predicts developmental potential of day 3 human embryos produced by IVF

Yang, Shuting; Shi, Junchao; Gong, Fei; Zhang, S.P.; Lu, Can‐Zhong; Tan, Ke; Leng, Lizhi; Hao, Mai; He, Hua; Gu, Yifan; Lu, Guangxiu; Lin, Ge

doi:10.1016/j.rbmo.2015.02.008

Cited by 56 publications

(46 citation statements)

References 60 publications

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“…In addition to these morphokinetic variables other studies (11)(12)(13)(14)(15)(16) focused on qualitative markers of cleavage-stage embryos that were associated with a reduced implantation potential and could be used as deselection criteria such as direct cleavage, multinucleation, and abnormal cleavage patterns. In a recently published study (17) another negative prognostic marker emerged, but in a blastocyst-stage embryo.…”

mentioning

confidence: 99%

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Bodri¹,

Sugimoto²,

Serna³

et al. 2016

Fertility and Sterility

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mentioning

confidence: 99%

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Bodri¹,

Sugimoto²,

Serna³

et al. 2016

Fertility and Sterility

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“…In the treatment cycle, nearly all the patient's own fertilized oocytes presented erratic cleavage patterns with distorted cytoplasmic movements, resulting in severe fragmentation. Distorted cytoplasmic movements at cleavage have been reported in two studies [34,35], and both revealed the poor prognoses of embryos exhibiting this phenomenon. After PB1T with cryopreserved PB1s, four of the seven 2PN zygotes were free of erratic cleavage patterns, and all zygotes developed into blastocysts with minimal fragmentation.…”

Section: Discussionmentioning

confidence: 94%

Polar body transfer restores the developmental potential of oocytes to blastocyst stage in a case of repeated embryo fragmentation

Zhang

Gong

et al. 2017

J Assist Reprod Genet

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Purpose We aimed to determine the developmental potential of human reconstructed oocytes after polar body genome transfer (PBT) and to report the case of a woman with multiple cycles of severe embryo fragmentation. Methods Fresh and cryopreserved first polar bodies (PB1s) were transferred to enucleated metaphase II oocytes (PB1T), while fresh PB2s were removed from fertilized oocytes and used instead of the female pronucleus in donor zygotes. Reconstructed oocytes underwent intracytoplasmic sperm injection (ICSI) and were cultured to blastocyst. Biopsied trophectoderm cells of PBT-derived blastocysts were screened for chromosomes by next-generation sequencing (NGS). Then, cryopreserved PB1T was carried out in one woman with a history of several cycles of extensive embryo fragmentation, and the blastocysts derived from PB1T were screened for aneuploidy but not transferred to the patient.Results There were no significant differences in the rates of normal fertilization and blastocyst formation between fresh and cryopreserved PB1T and control oocytes. Of the three fresh and three cryopreserved PB1T-derived blastocysts, two and one blastocysts exhibited normal diploidy respectively. In contrast, 17 PB2 transfers yielded 16 two pronuclei (2PN) zygotes with one normal and one small-sized pronucleus each and no blastocyst formation. In the female patient, 18 oocytes were inseminated by ICSI in the fourth cycle and the PB1s were biopsied. Although the embryos developed from the patient's own oocytes showed severe fragmentation, the oocytes reconstructed after PB1T produced three chromosomally normal blastocysts. Conclusions Normal blastocysts can develop from human reconstructed oocytes after PB1T. The application of the first PB transfers may be beneficial to patients with a history of poor embryo development and excessive fragmentation.

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“…Kelly et al observed two independent types of atypical cleavage (AC1 and AC2) and transferred 27 AC embryos, but only two implanted [9]. More recently, Yang et al reported that abnormal cleavage accounted for a 100 % influence on blastocyst formation [14]. However, the previously mentioned study did not describe the final pregnancy outcome.…”

Section: Discussionmentioning

confidence: 99%

Abnormally cleaving embryos are able to produce live births: a time-lapse study

Fan

Han

et al. 2016

J Assist Reprod Genet

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Purpose This study investigated the prevalence of abnormally cleaved embryos and determined which types of abnormally cleaved embryos (1-3c, 2-4c, 3-5c, 4-6c), might be suitable for transfer based on live birth data. Methods One hundred seventy-one women (whose transferred embryos were confirmed to be either fully implanted or fully unimplanted) provided 1256 embryos, which were analyzed. Results Of these embryos, 320 embryos were transferred, of these transferred embryos, 291 embryos were normal and 29 embryos were abnormal, which five embryos were not analyzed because each one was presented one abnormal cleavage type. These 24 embryos were divided into four groups. Inclusion criteria were as follows: women under 37 years of age undergoing first fresh in vitro fertilization (IVF) treatment with a basal antral follicle count of 5-15, body mass index (BMI) of 18-25 kg/m 2 , number of retrieved oocytes between 5 and 20, and tubal factors as the cause of infertility. Timelapse imaging analysis software was used to compare temporal parameters of normal cleavage and abnormal cleavage groups (there were four abnormal groups, based on the prevalence of abnormal cleavage embryos). Cleavage times were analyzed before the abnormal cleavage occurred, and time intervals were analyzed after the abnormal cleavage based upon the types of abnormal cleavage. In addition, the time intervals of t4-t3 and t8-t5 were also analyzed; corresponding time parameters were measured in the normal group as well. Implantation rate, clinical pregnancy rate, ongoing pregnancy rate, and live birth rate were also measured in the normally cleaved and abnormally cleaved embryos. The prevalence of abnormal cleavage was 15.92 % (200/1256). T8-t5 was the most important parameter in the prediction of potential development (production of a live-born baby) of abnormally cleaving embryos. Conclusions Abnormally cleaving embryos were able to produced live births with T8-t5 the best parameter to predict the developmental potential of abnormally cleaving embryos.

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Cleavage pattern predicts developmental potential of day 3 human embryos produced by IVF

Cited by 56 publications

References 60 publications

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Blastocyst collapse is not an independent predictor of reduced live birth: a time-lapse study

Polar body transfer restores the developmental potential of oocytes to blastocyst stage in a case of repeated embryo fragmentation

Abnormally cleaving embryos are able to produce live births: a time-lapse study

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