CLEC5A promotes the proliferation of gastric cancer cells by activating the PI3K/AKT/mTOR pathway

Wang, Quhui; Shi, Muqi; Sun, Shiqi; Zhou, Quan; Li, Ding; Jiang, Chenxia; Bian, Tingting; Feng, Jia; Liu, Yifei; Qin, Jun

doi:10.1016/j.bbrc.2019.10.122

Cited by 24 publications

(26 citation statements)

References 29 publications

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“…Hsa_circ_0010882 also activates PI3K/ Akt signaling and promotes gastric cancer development 25 . Previous studies p‐PI3K can activate Akt to form p‐Akt, and then participate in the process of gastric cancer development and development 26‐30 . It was shown p‐PI3K and p‐Akt were reduced after Herceptin treatment in gastric cancer cells, and hsa_circ_0000520 overexpression could restrain p‐PI3K and p‐Akt level.…”

Section: Discussionmentioning

confidence: 91%

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

Wang

et al. 2020

Clinical Laboratory Analysis

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Background To investigate whether hsa_circ_0000520 affects Herceptin resistance in gastric cancer by regulating the PI3K‐AKT signaling. Methods The expression of hsa_circ_0000520 was detected by qRT‐PCR in gastric cancer tissues and cell lines. A Herceptin‐resistant gastric cancer cell was established. PcDNA and pcDNA‐hsa_circ_0000520 were transfected into NCI‐N87R cells and treated with Herceptin at a concentration of 10 μg/mL for 24 hours. MTT tested cell proliferation, and apoptosis was measured by flow cytometry. IGF‐1 treatment was used to activate PI3K‐Akt signaling. The expression levels of related proteins were detected. Results The expression of hsa_circ_0000520 was reduced in gastric cancer tissues and cell lines, and hsa_circ_0000520 in NCI‐N87R cells was significantly lower than that of NCI‐N87 cells. Compared with the CON group, the cell viability of the Herceptin group was significantly reduced, the apoptosis rate was significantly increased, the level of Bax protein was significantly increased, and the levels of Bcl‐2, p‐PI3K, and p‐Akt protein were significantly reduced. Compared with the Herceptin + pcDNA group, the cell viability of the Herceptin + hsa_circ_0000520 group was significantly reduced, the apoptosis rate was significantly increased, the level of Bax protein was significantly increased, and the levels of p‐PI3K and p‐Akt proteins were significantly reduced. After IGF‐1 treatment, the cell viability was significantly increased, the apoptosis rate was significantly reduced, the level of Bax protein was significantly reduced, and the level of Bcl‐2 protein was significantly increased. Conclusion Hsa_circ_0000520 overexpression may reverse the Herceptin resistance of gastric cancer cells by inhibiting the PI3K‐Akt signaling pathway.

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Section: Discussionmentioning

confidence: 91%

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

Wang

et al. 2020

Clinical Laboratory Analysis

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“…LMO4 belongs to the LIM-only family of transcriptional coregulatory proteins and consists of two LIM protein-protein interaction domains that act as connexins in multiprotein complexes [21]. Sequence analysis of the mouse LMO4 gene revealed that it spans approximately 18 kb and consists of at least six exons, including two alternatively spliced 5′ exons.…”

Section: Discussionmentioning

confidence: 99%

Research and Clinical Significance of the Differentially Expressed Genes TP63 and LMO4 in Human Immunodeficiency Virus-related Penile Squamous Cell Carcinoma

Xue

Zheng

2021

Preprint

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Background: To study the differential gene expression and clinical significance in HIVIIs (human immunodeficiency virus-infected individuals) with penile squamous cell carcinoma.Methods: At our hospital from 2019 to 2020, we selected 6 samples of HIV-related penile squamous cell carcinoma for the experimental group and 6 samples of non-HIV-related penile squamous cell carcinoma for the control group. Transcriptome sequencing of sample mRNAs was performed by high-throughput sequencing. Differential gene expression analysis, differential GO (Gene Ontology) enrichment analysis and differential KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis were carried out, and the RPKM (reads per kilobase per million reads) value was used as a measure of gene expression.Results: A total of 2418 differentially expressed genes were obtained, of which 663 were upregulated and 1755 were downregulated (absolute value of logFC >1.0 and p value<0.05 FDR < 0.05). On the basis of the significance of the GO enrichment analysis, we found that the TP63 (tumor protein p63) gene was significantly upregulated and that the LMO4 (LIM domain only 4) gene was significantly downregulated in the experimental group compared with the control group. KEGG pathway analysis of the differentially expressed genes revealed that DNA replication was the most significant pathway associated with the upregulated genes and CAM (cell adhesion molecule) metabolism was the most significant pathway associated with the downregulated genes.Conclusions: The gene expression profiles of HIV-related penile squamous cell carcinoma and non-HIV-related penile squamous cell carcinoma are significantly different and involve significant GO enrichment and KEGG metabolic pathways, and this is very meaningful for the study of NADCs (non-AIDS-defining cancers). Differential expression of genes may be an important target for the prevention of penile squamous cell carcinoma in HIVIIs.

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“…LMO4 was indicated to excessively express in liver cancer tissues and cells. A conducted research has elucidated that LMO4 is overexpressed in gastric cancer tissues and LMO4 inhibition discourages cell aggressive behaviors 29 . Experimentally, LMO4 expression is raised in Burkitt lymphoma cells and LMO4 depression impairs the cell ability of migrating and accelerates cell apoptosis 30 .…”

Section: Discussionmentioning

confidence: 99%

“…A conducted research has elucidated that LMO4 is overexpressed in gastric cancer tissues and LMO4 inhibition discourages cell aggressive behaviors. 29 Experimentally, LMO4 expression is raised in Burkitt lymphoma cells and LMO4 depression impairs the cell ability of migrating and accelerates cell apoptosis. 30 What is more, it is displayed that LMO4 maintains a high expression in nonsmall cell lung cancer cells and tissues, which is involved in disciplining cell migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA small nucleolar RNA host gene 15 deteriorates liver cancer via microRNA‐18b‐5p/LIM‐only 4 axis

et al. 2020

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Extensive studies have explored the involvements of long noncoding RNAs (lncRNAs) in liver cancer. Limitedly, the concrete function of lncRNA small nucleolar RNA host gene 15 (SNHG15) is still elusive. Therefore, the work was initiated to unearth SNHG15-oriented mechanism in liver cancer. Liver cancer tissues were resected. The connection between SNHG15 expression with prognosis and clinicopathological traits of liver cancer patients was evaluated. Liver cancer cells SMMC-7721 were transfected with restored microRNA (miR)-18b-5p or depleted SNHG15 to discover their effects on the proliferation, migration, invasion, cycle arrest, and apoptosis of SMMC-7721 cells. The transfected SMMC-7721 cells were injected into nude mice for further investigation. SNHG15, miR-18b-5p, and LIM-only 4 (LMO4) expressions in tissues and cells were tested. The regulatory connections among SNHG15, miR-18b-5p, and LMO4 were detected. SNHG15 and LMO4 were overexpressed while miR-18b-5p was downregulated in liver cancer tissues and cells. Up-regulated SNHG15 was connected with inferior prognosis and aggressive behaviors of liver cancer patients. SNHG15 knockdown or miR-18b-5p restoration depressed SMMC-7721 cell growth in vivo and in vitro. SNHG15 bound to miR-18b-5p and miR-18b-5p targeted LMO4. The work has illuminated that silencing SNHG15 represses liver cancer progression by modulating miR-18b-5p and LMO4, indicating the therapeutic potency of SNHG15/miR-18b-5p/LMO4 axis in liver cancer.

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CLEC5A promotes the proliferation of gastric cancer cells by activating the PI3K/AKT/mTOR pathway

Cited by 24 publications

References 29 publications

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

hsa_circ_0000520 influences herceptin resistance in gastric cancer cells through PI3K‐Akt signaling pathway

Research and Clinical Significance of the Differentially Expressed Genes TP63 and LMO4 in Human Immunodeficiency Virus-related Penile Squamous Cell Carcinoma

Long noncoding RNA small nucleolar RNA host gene 15 deteriorates liver cancer via microRNA‐18b‐5p/LIM‐only 4 axis

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