Clenbuterol residue analysis by HPLC-HPTLC in urine and animal tissues

Degroodt, Jean-Marie; Bukanski, B. Wyhowski De; Beernaert, Hedwig; Courtheyn, D.

doi:10.1007/bf01332946

Cited by 28 publications

(6 citation statements)

References 11 publications

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“…Extraction and clean-up was based on a previously published method (Degroodt et al 1989). N-AB 760 CL (4-amino-a-[(l,l-dimethylpropylamino)-methyl]-3,5dichloro-benzyl alcohol hydrochloride) was donated by Boehringer Ingelheim (Bracknell, UK) and was incorporated prior to enzyme digestion as an internal standard to give a measure of recovery for each extract.…”

Section: Methodsmentioning

confidence: 99%

The effect of cooking on veterinary drug residues in food: 1. clenbuterol

Rose

Shearer

Farrington

1995

Food Additives and Contaminants

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The heat stability of clenbuterol was investigated. The drug was shown to be stable in boiling water at 100 degrees C. In cooking oil at 260 degrees C, losses were observed, indicating a half-life of about 5 min. The effect of a range of cooking processes (boiling, roasting, frying, microwaving) on clenbuterol residues in fortified and incurred tissue was studied. No net change in the amount of clenbuterol was observed in any of the cooking processes investigated except for deep frying using extreme conditions. There was little observed migration from the tissue into the surrounding liquid or meat juices. Clenbuterol residues were found not to be evenly distributed in the incurred raw tissue used for the investigation. The findings of this investigation show that data obtained from measurements on raw tissue are applicable for use in consumer exposure estimates and dietary intake calculations.

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Section: Methodsmentioning

confidence: 99%

The effect of cooking on veterinary drug residues in food: 1. clenbuterol

Rose

Shearer

Farrington

1995

Food Additives and Contaminants

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“…Tissue samples were weighed (500 ± 50 mg for liver and 1000 ± 100 mg for other tissues), cut, minced, homogenized, extracted and analyzed (Degroodt et al. , 1989; Uboh et al.…”

Section: Quantification Of Clenbuterolmentioning

confidence: 99%

Tissue distribution of clenbuterol in the horse

Soma¹,

Uboh²,

Guan³

et al. 2004

Vet Pharm & Therapeutics

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Plasma and tissue concentrations of clenbuterol (CLB) were determined following oral (p.o.) administration of 1.6 microg/kg twice daily (b.i.d.) for 2 weeks. Horses were administered the last dose on morning of day 15, killed at 0.25, 24, 48, and 72 h post-administration. At 0.25 h, the highest tissue concentrations of CLB were found in the liver (16.21 ng/g), lung (6.48 ng/g), left ventricle (4.99 ng/g), kidney (3.35 ng/g), bronchi (2.56 ng/g), right ventricle (2.08 ng/g), and eye fluids (1.09 ng/g) all of which were higher than that of plasma (1.10 ng/mL). The elimination half-lives (t(1/2k)) for CLB in tissues ranged from 21.2 to 56.3 h, the longest were in the eye fluids (56.9 h), spleen (21.2 h), cerebrum (27.1 h), cerebellum (21.5) and cecum (23.7 h). The t(1/2k) for plasma was 10.9 h. Tissue/plasma ratios of liver (14.7), lung (5.9), left ventricle (4.6), kidney (3.1), bronchi, (2.3) and right ventricle (1.9) were high at 0.25 h and remained elevated up to 72 h. Accumulation and sustained high concentration of CLB relative to plasma in these tissues contributed to the prolonged elimination and the ability to quantify CLB in plasma and urine for a prolonged period.

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“…The waterholding capacity of the meat was determined by measuring the percentage of water not set free out of a 0-3 g sample of the longissimus dorsi when a pressure of 1 kg was exterted for 15 min. Clenbuterol was analysed in a sample of the longissimus dorsi and the renal fat according to Degroodt et al (1989).…”

Section: Carcass and Meat Characteristicsmentioning

confidence: 99%

Effects of short-term feeding of clenbuterol on nitrogen retention, performance and meat quality in finishing pigs

1991

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S U M M A R YAn experiment using 30 Belgian landrace finishing pigs was carried out, in 1989, at the University of Leuven, Belgium, to examine the effect of clenbuterol in the diet (1 mg/kg) on the repartitioning of nutrients and body composition. Clenbuterol was administered for 20 days preceding the week before slaughter. Fifteen animals were fed a diet containing the /?-agonist, and 15 other animals served as negative controls. Weight gain, feed conversion and N utilization improved during /?-agonist treatment. Removal of clenbuterol from the diet rapidly increased blood urea concentrations, indicating immediate, less efficient, N utilization. In the week before slaughter, the animals did not lose the extra weight gained in the period of clenbuterol feeding. Backfat thickness at slaughter was reduced by 15% in the animals fed clenbuterol. Dressing percentage and post mortem pH decline, colour and water-holding capacity in ham and the longissimus dorsi were not affected by dietary treatment. Clenbuterol was not detected in renal fat and the longissimus dorsi at slaughter.

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Clenbuterol residue analysis by HPLC-HPTLC in urine and animal tissues

Cited by 28 publications

References 11 publications

The effect of cooking on veterinary drug residues in food: 1. clenbuterol

The effect of cooking on veterinary drug residues in food: 1. clenbuterol

Tissue distribution of clenbuterol in the horse

Effects of short-term feeding of clenbuterol on nitrogen retention, performance and meat quality in finishing pigs

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