Click and bioorthogonal hyaluronic acid hydrogels as an ultra-tunable platform for the investigation of cell-material interactions

Lagneau, Nathan; Tournier, Pierre; Halgand, Boris; Loll, François; Maugars, Y.; Guicheux, Jérôme; Visage, Catherine Le; Delplace, Vianney

doi:10.1016/j.bioactmat.2022.12.022

Cited by 11 publications

(15 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polysaccharide Modifications-Synthesis of HA-N 3 and CS-N 3 : N 3 : Modified polysaccharides were synthesized as previously described with minor modifications. [31] Briefly, 100 mg of HA (20 kDa) or CS was dissolved at 10 mg.mL −1 in MES buffer (pH = 5.5) at room temperature while stirring. After complete dissolution, DMT-MM (69 mg [1 eq] for HA; 60.2 mg [1 eq] for CS) was added and allowed to react for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…The degrees of substitution of HA-N 3 and CS-N 3 were determined by 1 H NMR (400 MHz, D 2 O): the N-acetyl group of HA or CS (3 protons at 2.1 ppm) served as a reference to quantify the relative amount of grafted N 3 (13 protons between 3.3 and 4.1 ppm), as previously reported. [31] Polysaccharide Modifications-Synthesis of Gelatin-N 3 : 50 mg of gelatin was dissolved at 10 mg.mL −1 in MES buffer (pH = 5.5) at room temperature while stirring. After complete dissolution, 10 mg of DMT-MM was added and allowed to react for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Regarding the second reaction, we investigated the use of strain-promoted azide-alkyne cycloaddition (SPAAC) [24] as a click and bioorthogonal reaction [5] able to meet all of the criteria for post-bioprinting modifications, including feasibility under physiological pH and temperature, and the absence of byproducts. [31] To demonstrate the potential of the proposed biomaterials, which we termed "clickable dynamic bioinks" (Figure 1), we performed a variety of post-printing modifications, including adjusting the polymer composition (e.g., HA, chondroitin sulfate [CS], etc.) and stiffness, and promoting cell adhesion with adhesive macromolecules (e.g., gelatin and RGD peptide).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Clickable Dynamic Bioinks Enable Post‐Printing Modifications of Construct Composition and Mechanical Properties Controlled over Time and Space

Tournier,

Saint‐Pé,

Lagneau

et al. 2023

Advanced Science

Self Cite

View full text Add to dashboard Cite

Bioprinting is a booming technology, with numerous applications in tissue engineering and regenerative medicine. However, most biomaterials designed for bioprinting depend on the use of sacrificial baths and/or non‐physiological stimuli. Printable biomaterials also often lack tunability in terms of their composition and mechanical properties. To address these challenges, the authors introduce a new biomaterial concept that they have termed “clickable dynamic bioinks”. These bioinks use dynamic hydrogels that can be printed, as well as chemically modified via click reactions to fine‐tune the physical and biochemical properties of printed objects after printing. Specifically, using hyaluronic acid (HA) as a polymer of interest, the authors investigate the use of a boronate ester‐based crosslinking reaction to produce dynamic hydrogels that are printable and cytocompatible, allowing for bioprinting. The resulting dynamic bioinks are chemically modified with bioorthogonal click moieties to allow for a variety of post‐printing modifications with molecules carrying the complementary click function. As proofs of concept, the authors perform various post‐printing modifications, including adjusting polymer composition (e.g., HA, chondroitin sulfate, and gelatin) and stiffness, and promoting cell adhesion via adhesive peptide immobilization (i.e., RGD peptide). The results also demonstrate that these modifications can be controlled over time and space, paving the way for 4D bioprinting applications.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Clickable Dynamic Bioinks Enable Post‐Printing Modifications of Construct Composition and Mechanical Properties Controlled over Time and Space

Tournier,

Saint‐Pé,

Lagneau

et al. 2023

Advanced Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another HA-based hydrogel was designed by Lagneau et al using the click and SPAAC reaction for encapsulating human adipose-derived stromal cells. [67] This led to enhanced secretion of key immunomodulatory factors, providing a platform for material-assisted cell therapies. More recently, Luo et al developed an injectable bioorthogonal hydrogel (BIOGEL) system that accelerates tissue regeneration in degenerated intervertebral discs.…”

Section: Bio-compatible Hydrogelsmentioning

confidence: 99%

“…Another HA‐based hydrogel was designed by Lagneau et al. using the click and SPAAC reaction for encapsulating human adipose‐derived stromal cells [67] . This led to enhanced secretion of key immunomodulatory factors, providing a platform for material‐assisted cell therapies.…”

Section: Bio‐compatible Hydrogelsmentioning

confidence: 99%

Bioorthogonal Chemistry in Translational Research: Advances and Opportunities

Chaudhuri,

Bhattacharya,

Dash

2023

ChemBioChem

View full text Add to dashboard Cite

Bioorthogonal chemistry is a rapidly expanding field of research that involves the use of small molecules that can react selectively with biomolecules in living cells and organisms, without causing any harm or interference with native biochemical processes. It has made significant contributions to the field of biology and medicine by enabling selective labeling, imaging, drug targeting, and manipulation of bio‐macromolecules in living systems. This approach offers numerous advantages over traditional chemistry‐based methods, including high specificity, compatibility with biological systems, and minimal interference with biological processes. In this review, we provide an overview of the recent advancements in bioorthogonal chemistry and their current and potential applications in translational research. We present an update on this innovative chemical approach that has been utilized in cells and living systems during the last five years for biomedical applications. We also highlight the nucleic acid‐templated synthesis of small molecules by using bioorthogonal chemistry. Overall, bioorthogonal chemistry provides a powerful toolset for studying and manipulating complex biological systems, and holds great potential for advancing translational research.

show abstract

Fish Collagen Cross‐Linking Strategies to Improve Mechanical and Bioactive Capabilities for Tissue Engineering and Regenerative Medicine

Leonard,

Cumming,

Ali

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Collagen is the most abundant protein found in humans and is fundamental to tissue structure and function. Collagen products used in biomedical research are primarily derived from mammals, and despite being mainly responsible for providing strength to native connective tissue, collagen hydrogels have comparatively low mechanical properties without the use of additional cross‐linking strategies. Alternative sources of collagen, like fish collagen, are emerging as key biomaterials in tissue engineering and regenerative medicine (TERM). By addressing cultural/religious concerns, ease of extraction, absence of mammalian‐derived allergens, and retention of functional motifs, fish collagen has many promising characteristics that make it a suitable alternative to mammalian collagen. Several physical and chemical cross‐linking strategies of fish collagen are explored to create more stable and resilient scaffolds for a variety of TERM applications. This comprehensive review explores how these modifications are optimized in fish collagen hydrogel systems. Herein, the use of fish collagen and their reported sources for TERM research, as well as the types of treatments (including sterilization) used to alter collagen structures and functions, are presented to date.

show abstract

Click and bioorthogonal hyaluronic acid hydrogels as an ultra-tunable platform for the investigation of cell-material interactions

Cited by 11 publications

References 59 publications

Clickable Dynamic Bioinks Enable Post‐Printing Modifications of Construct Composition and Mechanical Properties Controlled over Time and Space

Clickable Dynamic Bioinks Enable Post‐Printing Modifications of Construct Composition and Mechanical Properties Controlled over Time and Space

Bioorthogonal Chemistry in Translational Research: Advances and Opportunities

Fish Collagen Cross‐Linking Strategies to Improve Mechanical and Bioactive Capabilities for Tissue Engineering and Regenerative Medicine

Contact Info

Product

Resources

About