Click aspartic acid as H ILIC SPE material for selective enrichment of N-linked glycopeptides

Li, Xiuling; Shen, Guobin; Zhang, FeiFang; Yang, Bingcheng; Liang, Xinmiao

doi:10.1016/j.jchromb.2013.10.003

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…116 HILIC is not only used for analytical separations, but often also for SPE sample preparation, especially for the enrichment of glycopeptides. 117,118,119 The increased interest in the application of HILIC for glycomics analyses has further sparked the development of novel HILIC materials. Especially zwitterionic hydrophilic interaction chromatography (ZIC-HILIC), whereby both positively and negatively charged groups are attached to the analytical surface resulting in a relatively weaker interaction with charged analytes, has been reported in glycan analysis.…”

Section: Separationsmentioning

confidence: 99%

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

Kailemia¹,

et al. 2013

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Section: Separationsmentioning

confidence: 99%

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

Kailemia¹,

et al. 2013

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“…This material was used as SP extraction sorbent for selective enrichment of glycopeptides. 171 Another example reported was the building of 2-oxoglutaric acid receptor attached to resin via CuAAC-SP. This scaffold showed high affinity and specificity to separate the established 2-OG binding protein NtcA.…”

Section: Cu(i)-catalyzed Azide−alkynementioning

confidence: 99%

“…New glyco-silicas have shown to be efficient and valuable bioselective affinity chromatographic supports for the purification of lectins, as well as for the one-pot fluorescent labeling of these proteins . Yang et al developed a method, namely hydrophilic interaction chromatography (HILIC), which used a support prepared by clicking aspartic acid onto silica gel (termed as Click AA). This material was used as SP extraction sorbent for selective enrichment of glycopeptides .…”

Section: Cu(i)-catalyzed Azide–alkyne Cycloaddition On Solid-phase (...mentioning

confidence: 99%

CuAAC: An Efficient Click Chemistry Reaction on Solid Phase

2015

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Click chemistry is an approach that uses efficient and reliable reactions, such as Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC), to bind two molecular building blocks. CuAAC has broad applications in medicinal chemistry and other fields of chemistry. This review describes the general features and applications of CuAAC in solid-phase synthesis (CuAAC-SP), highlighting the suitability of this kind of reaction for peptides, nucleotides, small molecules, supramolecular structures, and polymers, among others. This versatile reaction is expected to become pivotal for meeting future challenges in solid-phase chemistry.

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“…The capture capabilities for the proteins were evaluated using a novel dual‐labelled BSA utilising fluorescence resonance energy transfer. Recently, a novel HILIC material was developed via clicking aspartic acid onto silica gel . The material demonstrated highly hydrophilic properties, and it was used as an SPE sorbent for the selective enrichment of glycopeptides possessing various peptide lengths and containing different types of glycans.…”

Section: Methods Based On Other Separation Techniquesmentioning

confidence: 99%

Stationary phases for the enrichment of glycoproteins and glycopeptides

et al. 2014

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The analysis of protein glycosylation is important for biomedical and biopharmaceutical research. Recent advances in LC-MS analysis have enabled the identification of glycosylation sites, the characterisation of glycan structures and the identification and quantification of glycoproteins and glycopeptides. However, this type of analysis remains challenging due to the low abundance of glycopeptides in complex protein digests, the microheterogeneity at glycosylation sites, ion suppression effects and the competition for ionisation by co-eluting peptides. Specific sample preparation is necessary for comprehensive and site-specific glycosylation analyses using MS. Therefore, researchers continue to pursue new columns to broaden their applications. The current manuscript covers recent literature published from 2008 to 2013. The stationary phases containing various chemical bonding methods or ligands immobilisation strategies on solid supports that selectively enrich N-linked or sialylated N-glycopeptides are categorised with either physical or chemical modes of binding. These categories include lectin affinity, hydrophilic interactions, boronate affinity, titanium dioxide affinity, hydrazide chemistry and other separation techniques. This review should aid in better understanding the syntheses and physicochemical properties of each type of stationary phases for enriching glycoproteins and glycopeptides.

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Click aspartic acid as H ILIC SPE material for selective enrichment of N-linked glycopeptides

Cited by 11 publications

References 25 publications

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

Oligosaccharide Analysis by Mass Spectrometry: A Review of Recent Developments

CuAAC: An Efficient Click Chemistry Reaction on Solid Phase

Stationary phases for the enrichment of glycoproteins and glycopeptides

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