Click chemistry: a new facile and efficient strategy for the preparation of Fe3O4 nanoparticles covalently functionalized with IDA-Cu and their application in the depletion of abundant protein in blood samples

Jian, Gui-Qin; Yu-xing, Liu; He, Xiwen; Chen, Langxing; Zhang, Yukui

doi:10.1039/c2nr31430d

Cited by 65 publications

(41 citation statements)

References 41 publications

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“…Hydrophilic interaction liquid chromatography (HILIC) approaches have shown to be simple, fast, and efficient relative to reversed-phase approaches for glycopeptide enrichment [19][20][21][22]. Much emphasis has been placed around the design of niche Bglyco-modified^columns usable under HILIC conditions including Bclick saccharides^onto polymer beads [23], silica beads [24,25], and magnetic nanoparticles [26].…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids

et al. 2015

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Metabolic engineering of glycans present on antibodies and other glycoproteins is becoming an interesting research area for improving our understanding of the glycome. With knowledge of the sialic acid biosynthetic pathways, the experiments described in this report are based on a published procedure involving the addition of a synthesized azido-mannosamine sugar into cell culture media and evaluation of downstream expression as azido-sialic acid. This unique bioorthogonal sugar has the potential for a variety of "click chemistry" reactions through the azide linkage, which allow for it to be isolated and quantified given the choice of label. In this report, mass spectrometry was used to investigate and optimize the cellular absorption of peracetylated N-azidoacetylmannosamine (Ac4ManNAz) to form N-azidoacetylneuraminic acid (SiaNAz) in a Chinese hamster ovary (CHO) cell line transiently expressing a double mutant trastuzumab (TZMm2), human galactosyltransferase 1 (GT), and human α-2,6-sialyltransferase (ST6). This in vivo approach is compared to in vitro enzymatic addition SiaNAz onto TZMm2 using soluble β-galactosamide α-2,6-sialyltransferase 1 and CMP-SiaNAz as donor. The in vivo results suggest that for this mAb, concentrations above 100 μM of Ac4ManNAz are necessary to allow for observation of terminal SiaNAz on tryptic peptides of TZMm2 by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. This is further confirmed by a parallel study on the production of EG2-hFc monoclonal antibody (Zhang J et al. Prot Expr Purific 65(1); 77-82, 2009) in the presence of increasing concentrations of Ac4ManNAz.

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Section: Introductionmentioning

confidence: 99%

Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids

et al. 2015

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“…While these methods are based on solid phase extraction (SPE), it is worthwhile noting their compatibility towards on-line analysis with techniques such as electrospray, where mobile phases used in HILIC are often advantageous due to the higher percentage of organic solvents which are desolvated efficiently [18]. In light of this, much emphasis has been placed around designing niche 'glyco-modified' columns which can be used under HILIC conditions including 'click saccharides' onto polymer beads [19], silica beads [20,21], and magnetic nanoparticles [22].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and evaluation of carboxymethyl chitosan for glycopeptide enrichment

Bodnar

Perreault

2015

Analytica Chimica Acta

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“…For example, Chen et al [19, 20] fabricated MNPs coated with iminodiacetic acid and charged with Cu 2+ or Ni 2+ to selectively adsorb histidine-rich bovine hemoglobin (BHb). BHb was also selectively captured on Cu 2+ -EDTA-Fe 3 O 4 magnetic particles synthesized by Ding et al through a one-pot solvothermal method [21].…”

Section: Introductionmentioning

confidence: 99%

Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles

Liu

Wang

Yan

et al. 2017

BioMed Research International

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Hierarchical copper shells anchored on magnetic nanoparticles were designed and fabricated to selectively deplete hemoglobin from human blood by immobilized metal affinity chromatography. Briefly, CoFe2O4 nanoparticles coated with polyacrylic acid were first synthesized by a one-pot solvothermal method. Hierarchical copper shells were then deposited by immobilizing Cu2+ on nanoparticles and subsequently by reducing between the solid CoFe2O4@COOH and copper solution with NaBH4. The resulting nanoparticles were characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. The particles were also tested against purified bovine hemoglobin over a range of pH, contact time, and initial protein concentration. Hemoglobin adsorption followed pseudo-second-order kinetics and reached equilibrium in 90 min. Isothermal data also fit the Langmuir model well, with calculated maximum adsorption capacity 666 mg g−1. Due to the high density of Cu2+ on the shell, the nanoparticles efficiently and selectively deplete hemoglobin from human blood. Taken together, the results demonstrate that the particles with hierarchical copper shells effectively remove abundant, histidine-rich proteins, such as hemoglobin from human blood, and thereby minimize interference in diagnostic and other assays.

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Click chemistry: a new facile and efficient strategy for the preparation of Fe3O4 nanoparticles covalently functionalized with IDA-Cu and their application in the depletion of abundant protein in blood samples

Cited by 65 publications

References 41 publications

Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids

Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids

Synthesis and evaluation of carboxymethyl chitosan for glycopeptide enrichment

Selective Removal of Hemoglobin from Blood Using Hierarchical Copper Shells Anchored to Magnetic Nanoparticles

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