Click Chemistry Immobilization of Antibodies on Polymer Coated Gold Nanoparticles

Finetti, Chiara; Solá, Laura; Pezzullo, Margherita; Prosperi, Davide; Colombo, Miriam; Riva, Beatrice; Avvakumova, Svetlana; Morasso, Carlo; Picciolini, Silvia; Chiari, Marcella

doi:10.1021/acs.langmuir.6b01142

Cited by 36 publications

(27 citation statements)

References 27 publications

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“…Silica or silica-coated particles with Si-OH groups on their surface were grafted with trialkoxysilanes with amine groups [ 214 , 215 ] and polymers containing trihydroxysilane moieties and clickable alkyne functions [ 216 ]. The particles with amine functions were further modified with 2-bromoisobutyryl bromide, yielding particles with ATRP initiator and/or with lanthanide-doped 4-cyano-4-((dodecylsulfanylthiocarbonyl) sulfanyl) pentanoic acid-produced particles with chain-transfer agent used in RAFT polymerizations.…”

Section: Preparation Of Functionalized Nano- and Microparticlesmentioning

confidence: 99%

Functionalized Particles Designed for Targeted Delivery

et al. 2021

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Pure bioactive compounds alone can only be exceptionally administered in medical treatment. Usually, drugs are produced as various forms of active compounds and auxiliary substances, combinations assuring the desired healing functions. One of the important drug forms is represented by a combination of active substances and particle-shaped polymer in the nano- or micrometer size range. The review describes recent progress in this field balanced with basic information. After a brief introduction, the paper presents a concise overview of polymers used as components of nano- and microparticle drug carriers. Thereafter, progress in direct synthesis of polymer particles with functional groups is discussed. A section is devoted to formation of particles by self-assembly of homo- and copolymer-bearing functional groups. Special attention is focused on modification of the primary functional groups introduced during particle preparation, including introduction of ligands promoting anchorage of particles onto the chosen living cell types by interactions with specific receptors present in cell membranes. Particular attention is focused on progress in methods suitable for preparation of particles loaded with bioactive substances. The review ends with a brief discussion of the still not answered questions and unsolved problems.

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Section: Preparation Of Functionalized Nano- and Microparticlesmentioning

confidence: 99%

Functionalized Particles Designed for Targeted Delivery

et al. 2021

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“…For example, Finetti et al successfully immobilized an azido modified anti-mouse IgG antibody on alkyne-functionalized gold nanoparticles via CuAAC click chemistry. [117] Due to the cytotoxicity of copper(I) catalyst, some copper-free click reactions have been also developed, such as strain-promoted azide-alkyne cycloaddition (SPAAC) where the alkyne part is a highly strained cyclic alkyne ( e.g. cyclooctyne).…”

Section: Antibody Immobilization Chemistriesmentioning

confidence: 99%

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

Shen

Rusling

Dixit

2017

Methods

162

109

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Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field. With all these new approaches at our disposal, several new immobilization methods have been created to address the main challenges associated with immobilized antibodies. Few of these challenges that we have discussed in this review are mainly associated to the site-specific immobilization, appropriate orientation, and activity retention. We have discussed the effect of antibody immobilization approaches on the parameters on the performance of an immunoassay.

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“…SeNPs also show favourable biocompatibility and can be easily conjugated with biomolecules without losing activity and preparing them is easy and cost effective ( Wang et al, 2019 ). Biomolecules such as antibodies can be immobilized on both AuNCs and SeNPs either passively through ionic or hydrophobic interactions or covalently through a chemical reaction with a surface group ( Finetti et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring optical spectroscopic techniques and nanomaterials for virus detection

Manoto

Elhussein

Malabi

et al. 2021

Saudi Journal of Biological Sciences

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Viral infections pose significant health challenges globally by affecting millions of people worldwide and consequently resulting in a negative impact on both socioeconomic development and health. Corona virus disease 2019 (COVID-19) is a clear example of how a virus can have a global impact in the society and has demonstrated the limitations of detection and diagnostic capabilities globally. Another virus which has posed serious threats to world health is the human immunodeficiency virus (HIV) which is a lentivirus of the retroviridae family responsible for causing acquired immunodeficiency syndrome (AIDS). Even though there has been a significant progress in the HIV biosensing over the past years, there is still a great need for the development of point of care (POC) biosensors that are affordable, robust, portable, easy to use and sensitive enough to provide accurate results to enable clinical decision making. The aim of this study was to present a proof of concept for detecting HIV-1 pseudoviruses by using anti-HIV1 gp41 antibodies as capturing antibodies. In our study, glass substrates were treated with a uniform layer of silane in order to immobilize HIV gp41 antibodies on their surfaces. Thereafter, the HIV pseudovirus was added to the treated substrates followed by addition of anti-HIV gp41 antibodies conjugated to selenium nanoparticle (SeNPs) and gold nanoclusters (AuNCs). The conjugation of SeNPs and AuNCs to anti-HIV gp41 antibodies was characterized using UV–vis spectroscopy, transmission electron microscopy (TEM) and zeta potential while the surface morphology was characterized by fluorescence microscopy, atomic force microscopy (AFM) and Raman spectroscopy. The UV–vis and zeta potential results showed that there was successful conjugation of SeNPs and AuNCs to anti-HIV gp41 antibodies and fluorescence microscopy showed that antibodies immobilized on glass substrates were able to capture intact HIV pseudoviruses. Furthermore, AFM also confirmed the capturing HIV pseudoviruses and we were able to differentiate between substrates with and without the HIV pseudoviruses. Raman spectroscopy confirmed the presence of biomolecules related to HIV and therefore this system has potential in HIV biosensing applications.

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Click Chemistry Immobilization of Antibodies on Polymer Coated Gold Nanoparticles

Cited by 36 publications

References 27 publications

Functionalized Particles Designed for Targeted Delivery

Functionalized Particles Designed for Targeted Delivery

Site-selective orientated immobilization of antibodies and conjugates for immunodiagnostics development

Exploring optical spectroscopic techniques and nanomaterials for virus detection

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