Clickable Microgel Scaffolds as Platforms for 3D Cell Encapsulation

Caldwell, Alexander S.; Campbell, Gavin T.; Shekiro, Kelly M. T.; Anseth, Kristi S.

doi:10.1002/adhm.201700254

Cited by 108 publications

(146 citation statements)

References 36 publications

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“…[185][186][187] Building on this, the Anseth lab has reported a microporous microgel scaffold composed of multiarmed PEG-based polymers crosslinked via reactive octyneazide chemistry. [188] The microgels were synthesized via an inverse suspension polymerization, demonstrating control over particle size by changing the rate of shear applied. This particle assembly technique shows great promise, especially in advancing the field of injectable biomaterials.…”

Section: Solvent/additive-free Hydrogelsmentioning

confidence: 99%

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Hoare

2017

Adv Healthcare Materials

178

168

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Structured macroporous hydrogels that have controllable porosities on both the nanoscale and the microscale offer both the swelling and interfacial properties of bulk hydrogels as well as the transport properties of “hard” macroporous materials. While a variety of techniques such as solvent casting, freeze drying, gas foaming, and phase separation have been developed to fabricate structured macroporous hydrogels, the typically weak mechanics and isotropic pore structures achieved as well as the required use of solvent/additives in the preparation process all limit the potential applications of these materials, particularly in biomedical contexts. This review highlights recent developments in the field of structured macroporous hydrogels aiming to increase network strength, create anisotropy and directionality within the networks, and utilize solvent‐free or additive‐free fabrication methods. Such functional materials are well suited for not only biomedical applications like tissue engineering and drug delivery but also selective filtration, environmental sorption, and the physical templating of secondary networks.

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Section: Solvent/additive-free Hydrogelsmentioning

confidence: 99%

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Hoare

2017

Adv Healthcare Materials

178

168

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“…We originally created microporous annealed particle (MAP) scaffolds as a way to combine microporosity and injectability, which showed a tremendous potential to heal wounds . We and others with similar technology have shown that in vitro, cells use these microporous scaffolds to expand and proliferate in three dimensions around the microparticles . These findings suggested that cells can be mixed with hydrogel immediately before delivery, and this can be used as a cellular delivery platform that can overcome some of the limitations of current hydrogel technologies.…”

mentioning

confidence: 99%

Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds

Koh

Griffin

Archang

et al. 2019

Small

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Delivery to the proper tissue compartment is a major obstacle hampering the potential of cellular therapeutics for medical conditions. Delivery of cells within biomaterials may improve localization, but traditional and newer void‐forming hydrogels must be made in advance with cells being added into the scaffold during the manufacturing process. Injectable, in situ cross‐linking microporous scaffolds are recently developed that demonstrate a remarkable ability to provide a matrix for cellular proliferation and growth in vitro in three dimensions. The ability of these scaffolds to deliver cells in vivo is currently unknown. Herein, it is shown that mesenchymal stem cells (MSCs) can be co‐injected locally with microparticle scaffolds assembled in situ immediately following injection. MSC delivery within a microporous scaffold enhances MSC retention subcutaneously when compared to cell delivery alone or delivery within traditional in situ cross‐linked nanoporous hydrogels. After two weeks, endothelial cells forming blood vessels are recruited to the scaffold and cells retaining the MSC marker CD29 remain viable within the scaffold. These findings highlight the utility of this approach in achieving localized delivery of stem cells through an injectable porous matrix while limiting obstacles of introducing cells within the scaffold manufacturing process.

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“…1E). Others and we have utilized MAP scaffolds for cell culture in vitro 24,[29][30][31][32][33][34][35] and support tissue ingrowth in vivo 24,[30][31][32] . HA-MAP scaffolds can be injected into the stroke core at 5-days post wounding without deforming the recipient hemisphere ( Fig.…”

Section: Characterization Of Map Hydrogel and Stroke Tissuementioning

confidence: 99%

Hyaluronic acid particle hydrogels decrease cerebral atrophy and promote pro-reparative astrocyte/axonal infiltration in the core after ischemic stroke

Sideris

Chen

et al. 2019

Preprint

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The death rate due to stroke is decreasing, resulting in more individuals living with stroke related disabilities. Following stroke, dying cells contribute to the large influx of highly reactive astrocytes and pro-inflammatory microglia that release cytokines and lead to a cytotoxic environment that causes further brain damage and prevents endogenous repair. Paradoxically, these same cells also activate pro-repair mechanisms that contribute to endogenous repair and brain plasticity. Here, we show that the direct injection of a hyaluronic acid based microporous annealed particle (HA-MAP) hydrogel into the stroke core reduces the percent of highly reactive astrocytes and increases the percent of alternatively activated microglia in and around the lesion. Further, we show that HA-MAP hydrogel promotes reparative astrocyte infiltration into the lesion, which directly coincides with axonal penetration into the lesion. Additionally, HA-MAP injection decreases cerebral atrophy and preserves nigrostriatal bundles after stroke. This work shows that the injection of a porous scaffold into the stroke core can lead to clinically relevant decrease in cerebral atrophy and modulates the phenotype of astrocytes and microglia towards a pro-repair phenotype.

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Clickable Microgel Scaffolds as Platforms for 3D Cell Encapsulation

Cited by 108 publications

References 36 publications

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Structured Macroporous Hydrogels: Progress, Challenges, and Opportunities

Enhanced In Vivo Delivery of Stem Cells using Microporous Annealed Particle Scaffolds

Hyaluronic acid particle hydrogels decrease cerebral atrophy and promote pro-reparative astrocyte/axonal infiltration in the core after ischemic stroke

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