Pesticide residues in agricultural practices pose significant risks to public health, particularly in agro-pastoral communities where knowledge of pesticide usage is often limited. This study assesses pesticide residue practices among agro-pastoralists in Niger State, Nigeria, and examines the associated public health implications. A cross-sectional survey was conducted across three agro-ecological zones (A, B, and C) using structured questionnaires. The survey targeted nomadic and sedentary pastoral cattle herds to gather data on pesticide usage, exposure, and risk factors. Results revealed widespread pesticide misuse, largely driven by poor regulatory enforcement, low educational levels, and increasing demand for agricultural productivity. Additionally, significant variations were observed in pesticide knowledge and practices between the zones. This study highlights the urgent need for targeted interventions, stricter regulatory controls, and educational programs to mitigate health risks and enhance compliance with international safety standards.