Climate change impact on fungi in the atmospheric microbiome

Hanson, Mary; Petch, Geoffrey; Ottosen, T-B; Skjøth, C.

doi:10.1016/j.scitotenv.2022.154491

Cited by 23 publications

(26 citation statements)

References 85 publications

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“…Airborne material was collected with two Burkard multi-vial cyclones in Worcestershire, UK; one rural site (52.2544°, − 2.2537°) and one urban (52.1969°, − 2.2422°). Material was collected for 10 weeks from the beginning of July during the years 2016-2018 (Hanson et al, 2022), hence providing 420 daily samples. DNA was extracted according to (Hanson et al, (2022) and pooled into 6 annual aggregations for each of the years 2016-2018.…”

Section: Methodsmentioning

confidence: 99%

“…Material was collected for 10 weeks from the beginning of July during the years 2016-2018 (Hanson et al, 2022), hence providing 420 daily samples. DNA was extracted according to (Hanson et al, (2022) and pooled into 6 annual aggregations for each of the years 2016-2018. Samples were profiled using the ITS2 region and Illumina MiSEQ sequencing was performed externally by Eurofins Genomics.…”

Section: Methodsmentioning

confidence: 99%

“…Samples were profiled using the ITS2 region and Illumina MiSEQ sequencing was performed externally by Eurofins Genomics. Bioinformatic analysis was performed in R with taxonomic assignment against the UNITE eukaryotic database v8.3 and using Dada2, phyloseq and vegan packages as detailed in Hanson et al (2022) and postprocessing of the Amplicon Sequence Variant (ASV) data for pollen producing plants (Anthophyta and Coniferophyta) was done by c-shell scripting. The most prevalent pollen producing plants at each site were determined at the genus level by examining the top ten taxa and total biodiversity was calculated by enumerating all possible taxa at the genus level.…”

Section: Methodsmentioning

confidence: 99%

“…The most prevalent pollen producing plants at each site were determined at the genus level by examining the top ten taxa and total biodiversity was calculated by enumerating all possible taxa at the genus level. ASV data were used to calculate alpha and beta diversities in a similar way as for spores in the study by Hanson et al (2022). For beta diversity we use nonmetric multidimensional scaling (NMDS) based on Bray-Curtis dissimilarity to study how different the rural and urban sites are in terms of pollen flora.…”

Section: Methodsmentioning

confidence: 99%

“…Pinus, Olea, Cupressaceae and Amaranthaceae) (Buters et al, 2018;Ziello et al, 2012); however, the combined number of taxa recorded in the European network, remains much lower compared to what is expected from next generation sequencing methods (de Vere et al, 2012). New approaches based on collection with cyclones, next generation sequencing and metabarcoding have shown to provide much more taxonomical detail (Brennan et al, 2019;Hanson et al, 2022) but these approaches are still so new, that it is unclear how much more detail it is possible to obtain. The purpose of this study is to analyse data from an urban and nearby rural site with next generation sequencing over a 10 week period over three consecutive years covering the period when grasses and weeds are flowering to test whether there is a significant difference between the airborne pollen spectrum at the genus level and to explore whether the molecular approach provides substantially more information about species diversity than the optical microscopy approach used in the operational network.…”

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confidence: 99%

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Summer pollen flora in rural and urban central England dominated by nettle, ryegrass and other pollen missed by the national aerobiological network

et al. 2022

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Abundance and diversity of airborne pollen are important to human health and biodiversity. The UK operational network collects airborne pollen from 8 flowering trees, grasses and three weeds using Hirst traps and microscopic identification from urban areas. Knowledge of total pollen diversity and differences between rural and urban zones is limited. We collect environmental DNA (eDNA) from air during summer and autumn over 3 years with mini cyclones from one urban and one rural site. Data are analysed using next generation sequencing and metabarcoding. We find the most common genus, Urtica (57%), is also identified by the national network. The grasses Lolium (10%), Agrostis (2%) and Holcus (1%) are in the national network grouped at family level, while Brassica (2%), Chenopodium (1%), Impatiens (2%), Plantago (4%) and Tilia (7%) are not part of the UK operational network. DNA from 138 genera was identified, where 2% of the sample could not be associated with specific genera. 40% of the sample was classified better using eDNA methods at the genus level, than by optical methods. We calculate Bray–Curtis dissimilarity for the rural and urban zones and find a systematic difference in biodiversity. Overall, this shows airborne DNA reveals more information than methods based on morphological differences. The results also suggest data from sites located in large urban areas will be less representative for less populated rural areas. This presents a dilemma in balancing a network and the associated costs delivering health relevant information to the most populated areas vs. a nation-wide approach.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Summer pollen flora in rural and urban central England dominated by nettle, ryegrass and other pollen missed by the national aerobiological network

et al. 2022

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Assessing global fungal threats to humans

2022

mLife

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Fungi are an integral part of the earth's biosphere. They are broadly distributed in all continents and ecosystems and play a diversity of roles. Here, I review our current understanding of fungal threats to humans and describe the major factors that contribute to various threats. Among the 140,000 or so known species out of the estimated six million fungal species on Earth, about 10% directly or indirectly threaten human health and welfare. Major threats include mushroom poisoning, fungal allergies, infections of crop plants, food contamination by mycotoxins, and mycoses in humans. A growing number of factors have been identified to impact various fungal threats, including human demographics, crop distributions, anthropogenic activities, pathogen dispersals, global climate change, and/or the applications of antifungal drugs and agricultural fungicides. However, while models have been developed for analyzing various processes of individual threats and threat managements, current data are primarily descriptive and incomplete, and there are significant obstacles to integration of the diverse factors into accurate quantitative assessments of fungal threats. With increasing technological advances and concerted efforts to track the spatial and temporal data on climate and environmental variables; mycotoxins in the feed and food supply chains; fungal population dynamics in crop fields, human and animal populations, and the environment; human population demographics; and the prevalence and severities of fungal allergies and diseases, our ability to accurately assess fungal threats will improve. Such improvements should help us develop holistic strategies to manage fungal threats in the future.

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Using qPCR and microscopy to assess the impact of harvesting and weather conditions on the relationship between Alternaria alternata and Alternaria spp. spores in rural and urban atmospheres

et al. 2023

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Alternaria is a plant pathogen and human allergen. Alternaria alternata is one of the most abundant fungal spores in the air. The purpose of this study was to examine whether Alternaria spp. spore concentrations can be used to predict the abundance and spatio-temporal pattern of A. alternata spores in the air. This was investigated by testing the hypothesis that A. alternata dominates airborne Alternaria spp. spores and varies spatio-temporally. Secondarily, we aimed at investigating the relationship between airborne Alternaria spp. spores and the DNA profile of A. alternata spores between two proximate (~ 7 km apart) sites. These were examined by sampling Alternaria spp. spores using Burkard 7-day and cyclone samplers for the period 2016–2018 at Worcester and Lakeside campuses of the University of Worcester, UK. Daily Alternaria spp. spores from the Burkard traps were identified using optical microscopy whilst A. alternata from the cyclone samples was detected and quantified using quantitative polymerase chain reaction (qPCR). The results showed that either A. alternata or other Alternaria species spores dominate the airborne Alternaria spore concentrations, generally depending on weather conditions. Furthermore, although Alternaria spp. spore concentrations were similar for the two proximate sites, A. alternata spore concentrations significantly varied for those sites and it is highly likely that the airborne samples contained large amounts of small fragments of A. alternata. Overall, the study shows that there is a higher abundance of airborne Alternaria allergen than reported by aerobiological networks and the majority is likely to be from spore and hyphal fragments.

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Climate change impact on fungi in the atmospheric microbiome

Cited by 23 publications

References 85 publications

Summer pollen flora in rural and urban central England dominated by nettle, ryegrass and other pollen missed by the national aerobiological network

Summer pollen flora in rural and urban central England dominated by nettle, ryegrass and other pollen missed by the national aerobiological network

Assessing global fungal threats to humans

Using qPCR and microscopy to assess the impact of harvesting and weather conditions on the relationship between Alternaria alternata and Alternaria spp. spores in rural and urban atmospheres

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