Land-use change and regional carbon emissions are closely related. In-depth research on the mechanism of land-use carbon emissions is conducive for clarifying the direction and focus of future low-carbon construction work. Carbon emissions calculation models were used to calculate total carbon emissions in Guizhou Province and reveal their spatiotemporal distribution characteristics. The results were as follows. (1) The land-use structure of Guizhou Province underwent a significant change from 2009 to 2019, in particular with regard to cultivated and construction land. (2) Land-use carbon emissions in Guizhou Province were increasing annually, and this was related to urbanization, population density, and energy consumption. (3) There were spatial differences in the regional distribution of carbon emissions, their intensity, and carbon emissions per capita in Guizhou Province. Additionally, there were spatial clusters of cities with high or low emissions intensity. Therefore, there is scope for constructive proposals concerning sustainable land use and development, such as controlling the total amount and intensity of construction land, optimizing the structure of land use, managing the rational distribution of the population, constantly advancing the optimization of industrial structure, and improving the relevant policies.