HE aim of this study was to elucidate the impact of administering kisspeptin through injection on gonadotropin profile (LH) in treated ewes, to evaluate the effect of CIDR and kisspeptin injection on ovarian cyclicity (estrus activity), pregnancy rate and fecundity in adult ewes. Twenty four local Awassi non-pregnant and non-lactating ewes with three adult Rams of proven fertility of (2-4) years old used in the study, the period of study was extended from March 2022 to December 2022. The Experimental animals, which were sheep in this case, were randomly divided into three groups, each consisting of eight ewe: The First group of control (G1), The control (G1), Estrus synchronization group (G2); utilizes CIDR for estrus synchronization for 12 days+ kisspeptin-10 at time of CIDR withdrawal, and the third group (G3) using only for 12 days. The investigation encompassed the examination of estrus synchronization and the subsequent natural mating of the animals with proven rams. Blood samples were collected 60 and 30 minutes prior to the administration of kisspeptin and CIDR withdrawal,and 15,30, 60, 90, 120, 180, 240, 300, and 360 min after kisspeptin injection. The results indicated that the response duration in G2 and G3 was 49.75 and 60.12 minutes, respectively. These findings revealed no significant differences in duration response. Additionally, there were no significant differences in conception rates between G2 and G3, with rates of 100% and 66.6%, respectively. In terms of lambing rates, G2 achieved 100% while G3 reached 66.6%. Fecundity rates were recorded as 137.5% for G2 and 83.3% for G3, respectively. Regarding the activity of LH, the concentration increase after (15) min of kisspeptin injection. In conclusion, treatment of Awassi ewes with Kisspeptin not effect on reproductive performance and significant affect serum LH concentrations.