Clinical and epidemiological aspects of the infection by Babesia, Theileria and Trypanosoma species in horses from northeastern Colombia

Jaimes-Dueñez, Jeiczon; Jiménez-Leaño, Ángela; Enrique-Niño, Samuel; Árias-Landazabal, Norberto; Bedoya-Ríos, Miguel; Rangel-Pachón, David

doi:10.1016/j.ttbdis.2023.102208

Cited by 3 publications

(1 citation statement)

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“…Clinical manifestations of A. marginale infection are marked by acute onset fever and other nonspecific symptoms such as decreased appetite and general weakness, similar to other insect-borne infectious blood diseases, such as, babesiosis caused by Babesia spp. and trypanosomiasis caused by Trypanosoma spp 13 – 15 . Hence, A. marginale infection is often misdiagnosed or diagnosed late in the disease state, and can only be confirmed by the identification of the infectious bacterium.…”

Section: Introductionmentioning

confidence: 99%

RPA-CRISPR/Cas12a assay for the diagnosis of bovine Anaplasma marginale infection

Sutipatanasomboon,

Wongsantichon,

Sakdee

et al. 2024

Sci Rep

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Anaplasma marginale infection is one of the most common tick-borne diseases, causing a substantial loss in the beef and dairy production industries. Once infected, the pathogen remains in the cattle for life, allowing the parasites to spread to healthy animals. Since clinical manifestations of anaplasmosis occur late in the disease, a sensitive, accurate, and affordable pathogen identification is crucial in preventing and controlling the infection. To this end, we developed an RPA-CRISPR/Cas12a assay specific to A. marginale infection in bovines targeting the msp4 gene. Our assay is performed at one moderately high temperature, producing fluorescent signals or positive readout of a lateral flow dipstick, which is as sensitive as conventional PCR-based DNA amplification. This RPA-CRISPR/Cas12a assay can detect as few as 4 copies/μl of Anaplasma using msp4 marker without cross-reactivity to other common bovine pathogens. Lyophilized components of the assay can be stored at room temperature for an extended period, indicating its potential for field diagnosis and low-resource settings of anaplasmosis in bovines.

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Section: Introductionmentioning

confidence: 99%