Clinical and forensic examinations of glycemic marker 1,5-anhydroglucitol by means of high performance liquid chromatography tandem mass spectrometry

Heß, Cornelius; Stratmann, Bernd; Quester, Wulf; Madea, Burkhard; Mußhoff, Frank; Tschöepe, Diethelm

doi:10.1016/j.forsciint.2012.05.010

Cited by 19 publications

(11 citation statements)

References 24 publications

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“…Nevertheless, concentrations in femoral blood < 10 mg/ml indicate a badly adjusted diabetes mellitus before and after death. [9] In the actually described cases concentrations were very low (3.15 mg/ml and lower).…”

Section: Discussionmentioning

confidence: 87%

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Detection of diabetic metabolism disorders post‐mortem – forensic case reports on cause of death hyperglycaemia

Heß

Wöllner

Mußhoff

et al. 2013

Drug Testing and Analysis

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Diabetic coma is the most severe form of hyperglycaemic metabolic disorders. The post-mortem diagnosis of this disorder of glucose metabolism can be difficult and vague due to a lack of characteristic morphological findings. Six death cases caused by diabetic coma are described with special focus on biochemical (and histological) findings. The possible glycaemia markers glucose, lactate, HbA1c, fructosamine, anhydroglucitol, and ketone bodies were measured and the usefulness of these parameters is evaluated and discussed. Estimations of glucose concentrations in vitreous humour or cerebrospinal fluid and of ketone bodies in blood or other matrices are obligatory while measurements of HbA1c, fructosamine, or anhydroglucitol can only provide additional information on the long-term adjustment of diabetes in the deceased. Lactate concentrations (addition of glucose and lactate levels to form the sum formula of Traub) do not give more information than the glucose concentration itself and can be therefore omitted.

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Section: Discussionmentioning

confidence: 87%

“…Anhydroglucitol was detected by a validated liquid chromatography mass spectrometry after a protocol published by Hess et al [9] at our institute. For sample preparation, briefly, 50 ml of serum were fortified with 10 ml of internal standard (200 mg/ml 1,5-Anhydro-D-[ 13 C 6 ] glucitol).…”

Section: Methodsmentioning

confidence: 99%

Detection of diabetic metabolism disorders post‐mortem – forensic case reports on cause of death hyperglycaemia

Heß

Wöllner

Mußhoff

et al. 2013

Drug Testing and Analysis

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“…We also observed that in the control group 1,5-AG concentration increased with the increase in time from death to sampling, which should, however, have no effect on determining hyperglycemia as cause of death. The importance of determining this marker in diagnosis of hyperglycemia is confirmed by Hess et al [9,11]. In studies carried out on serum samples collected from 315 patients (116 non-diabetics and 199 diabetics) and blood samples of 58 deceased (27 non-diabetics and 31 diabetics), they showed significantly lower levels in patients with diabetes.…”

Section: Discussionmentioning

confidence: 91%

“…Similarly, Takata et al [33] confirmed the usefulness of 1,5-AG determination in VH. In addition, Hess et al [11] demonstrated postmortem metabolic stability of 1,5-AG.…”

Section: Discussionmentioning

confidence: 99%

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Postmortem Determination of Short-Term Markers of Hyperglycemia for the Purposes of Medicolegal Opinions

Nowak

Zawadzki

2020

Diagnostics

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Diabetes mellitus is classified as the epidemic of the 21st century. Due to the fact that acute carbohydrate metabolism disorders usually do not indicate morphological change, postmortem diagnosis is required to perform biochemical tests. The authors decided to evaluate the usefulness of determining glucose, lactate, acetone, β-hydroxybutyric acid (BHB), and 1,5-anhydroglucitol (1,5-AG) in postmortem blood/serum, urine, and vitreous humor (VH). Biological material was collected during autopsies. The study group consisted of 50 diabetics, while the control group consisted of 50 non-diabetics, who died a sudden death, with negative test results for the presence of ethyl alcohol and were not resuscitated before death. Statistical analysis was performed using the IBM SPSS Statistics 25 software package. The most statistically significant difference between the two groups was observed for mean 1,5-AG concentration. The authors found many correlations between the concentration of the examined markers in different materials, mainly between blood/serum and VH. The most suitable short-term glycemic marker in postmortem diagnosis is 1,5-AG. Diagnosis may be supported with determinations of acetone and BHB. For medicolegal assessment, the interpretation of the biochemical test results should comprise information on circumstances of death, medical history, results of other toxicological and histopathological tests, and autopsy report.

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Effects of Diabetes on Fitness to Drive

Quester¹

2022

Handbook of Forensic Medicine

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Clinical and forensic examinations of glycemic marker 1,5-anhydroglucitol by means of high performance liquid chromatography tandem mass spectrometry

Cited by 19 publications

References 24 publications

Detection of diabetic metabolism disorders post‐mortem – forensic case reports on cause of death hyperglycaemia

Detection of diabetic metabolism disorders post‐mortem – forensic case reports on cause of death hyperglycaemia

Postmortem Determination of Short-Term Markers of Hyperglycemia for the Purposes of Medicolegal Opinions

Effects of Diabetes on Fitness to Drive

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