Clinical and Histological Assessment of Collagen-Induced Arthritis Progression in the Diabetes-Resistant BB/Wor Rat

Knoerzer, D B; Donovan, Matt G.; Schwartz, Benjamin D.; Mengle-Gaw, L.

doi:10.1177/019262339702500103

Cited by 17 publications

(6 citation statements)

References 19 publications

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“…Each limbs were examined by light microscopy for the extent of inflammation and joint destruction. The inflammation score was based on pannus formation, immune cell infiltration, and joint destruction (Knoerzer, Donovan, Schwartz, & Mengle‐Gaw, ). Sections were also stained with safranin‐O and toluidine blue to evaluate cartilage destruction.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effect of long‐interval repeated intravenous administration of human umbilical cord blood‐derived mesenchymal stem cells in DBA /1 mice with collagen‐induced arthritis

Yoon

Kang

et al. 2019

J Tissue Eng Regen Med

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Rheumatoid arthritis (RA) is a common inflammatory chronic disease. It has been reported that mesenchymal stem cells (MSCs) have the effect of immune suppression in collagen-induced arthritis (CIA) mice model. However, the in vivo therapeutic effect from the long-interval repeated intravenous administration of human umbilical cord blood-derived (hUCB)-MSCs had not been investigated in CIA mice model. This

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Section: Methodsmentioning

confidence: 99%

Therapeutic effect of long‐interval repeated intravenous administration of human umbilical cord blood‐derived mesenchymal stem cells in DBA /1 mice with collagen‐induced arthritis

Yoon

Kang

et al. 2019

J Tissue Eng Regen Med

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“…In the early-stage CIA, there is a mild infiltration of inflammatory cells into the joint and significant upregulation in the expression of key receptors and channels in the cell bodies of nociceptive fibres innervating the ankle joints that are indicative of sensory hypersensitivity [ 11 ]. No swelling of the fore paws or knee joints is observed, just ankle joint inflammation that starts to spread down to the paw [ 11 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Neuron-immune mechanisms contribute to pain in early stages of arthritis

Nieto

Clark

Grist

et al. 2016

J Neuroinflammation

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BackgroundRheumatoid arthritis (RA) patients frequently show weak correlations between the magnitude of pain and inflammation suggesting that mechanisms other than overt peripheral inflammation contribute to pain in RA. We assessed changes in microglial reactivity and spinal excitability and their contribution to pain-like behaviour in the early stages of collagen-induced arthritis (CIA) model.MethodsMechanically evoked hypersensitivity, spinal nociceptive withdrawal reflexes (NWRs) and hind paw swelling were evaluated in female Lewis rats before and until 13 days following collagen immunization. In the spinal dorsal horn, microgliosis was assayed using immunohistochemistry (Iba-1/p-p38) and cyto(chemo)kine levels in the cerebrospinal fluid (CSF). Intrathecal administration of microglia-targeting drugs A-438079 (P2X7 antagonist) and LHVS (cathepsin S inhibitor) were examined upon hypersensitivity, NWRs, microgliosis and cyto(chemo)kine levels in the early phase of CIA.ResultsThe early phase of CIA was associated with mechanical allodynia and exaggerated mechanically evoked spinal NWRs, evident before hind paw swelling, and exacerbated with the development of swelling. Concomitant with the development of hypersensitivity was the presence of reactive spinal microgliosis and an increase of IL-1β levels in CSF (just detectable in plasma). Prolonged intrathecal administration of microglial inhibitors attenuated the development of mechanical allodynia, reduced microgliosis and attenuated IL-1β increments. Acute spinal application of either microglial inhibitor significantly diminished the sensitization of the spinal NWRs.ConclusionsMechanical hypersensitivity in the early phase of CIA is associated with central sensitization that is dependent upon microglial-mediated release of IL-1β in the spinal cord. Blockade of these spinal events may provide pain relief in RA patients.

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“…The CAIA model is a model of an acute arthritis, represents the rapid onset of disease, and usually has a short experimental time period [ 2 ]. In contrast, CIA model represents the gradual onset of disease, and it usually requires an the experimental period of 4 weeks to show hypertrophy of the synovial lining and longer periods to show fibrovascular pannus formation [ 11 ]. The choice of the most proper model should be based on adequate research, and the biology of the animal model should be compared with the intended therapeutic target molecule.…”

Section: Discussionmentioning

confidence: 99%

Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination

Sim

Lee

et al. 2016

Lab Anim Res

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The aim of this study was to examine the therapeutic potential of sulfasalazine and prednisolone in a mouse collagen antibody-induced arthritis (CAIA) model. Twenty-five male BALB/c mice were randomly divided into five groups: group 1 (G1): control, group 2 (G2): probe control, group 3 (G3): CAIA, group 4 (G4): CAIA+sulfasalazine (10 mg/kg, oral), and group 5 (G5): CAIA+prednisolone (100 mg/kg, oral). Fluorescence bioimaging was performed in vivo 24 and 48 h after treatment with a fluorescence probe (OsteoSense® 680 EX), and all mice were sacrificed. The hind knee joints were fixed in 10% neutral phosphate-buffered formalin, and micro-computed tomography (micro-CT) and histopathological analyses were performed. The paw thickness increased in a time-dependent manner in G3 mice, but trended toward a decrease in both G4 and G5 mice. Fluorescence intensity increased in G3 mice at 24 and 48 h after fluorescence probe treatment, but the fluorescence intensity in G4 and G5 mice was lower than that in G3. Micro-CT analyses showed that the joint surfaces of G3 mice had a rough and irregular articular appearance, but the occurrence of these irregularities was lower in G4 and G5. Hematoxylin and eosin and Safranin O-fast green staining confirmed that destruction of the cartilage and bony structures, synovial hyperplasia, and inflammatory cell infiltration all occurred in G3, and that the occurrence of these phenomena was lower in G4 and G5 than in G3. Taken together, these results suggest that sulfasalazine and prednisolone can reduce acute rheumatoid arthritis in mice.

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Clinical and Histological Assessment of Collagen-Induced Arthritis Progression in the Diabetes-Resistant BB/Wor Rat

Cited by 17 publications

References 19 publications

Therapeutic effect of long‐interval repeated intravenous administration of human umbilical cord blood‐derived mesenchymal stem cells in DBA /1 mice with collagen‐induced arthritis

Therapeutic effect of long‐interval repeated intravenous administration of human umbilical cord blood‐derived mesenchymal stem cells in DBA /1 mice with collagen‐induced arthritis

Neuron-immune mechanisms contribute to pain in early stages of arthritis

Assessment of collagen antibody-induced arthritis in BALB/c mice using bioimaging analysis and histopathological examination

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