Clinical and Histopathological Studies on the Efficacy of Multiple Injections of I-PRF Versus the Single Use of A-PRF in Repair of Achilles Tendon Rupture in Dogs: An Experimental Study

Abstract

Platelets concentrates (PC) proved in many studies to enhance the healing process in different tissues including tendon healing as they produce large number of GFs and cytokines so the aim of the current study was to evaluate the efficacy of both Injectable-platelets rich fibrin (I-PRF) and Advancedplatelets rich fibrin (A-PRF) in enhancement of the healing of surgically treated AT rupture in dogs and compare the efficacy of the single administration of the A-PRF membranes with multiple injections of the I-PRF. Study design: Eight adult male mongrel dogs with average weight 18 kg ± 3 and with average age 2 years ± 1.5 were used in this study and were divided into two groups, A-PRF group: A-PRF membranes were applied during surgery only. I-PRF group: I-PRFwas injected during the surgery with 2 more booster injections with 3 weeks intervals after surgery. During observation period, dogs were observed clinically at 3,6, 8, 10-and 12-weeks post-operation and histologically at 6-and 12-weeks post-operation.Results: the clinical observation revealed a similar effect of the two groups at the early stages of the healing process while the I-PRF group showed significantly better healing of the tendon with decreased thickness and histological examination revealed significantly higher healing, arrangement of the fibers and collagen deposition in the I-PRF group.Conclusion: The present study revealed that A-PRF and I-PRF have the ability to enhance and accelerate the healing and regeneration processes of the Achilles tendon while the multiple injection of the I-PRF add a great value over the A-PRF group.