Clinical and Laboratory Observations in Cases of Triiodothyronine Toxicosis Confirmed by Radioimmunoassay

Hollander, Charles S.; Mitsuma, Terunori; Nihei, Noriyuki; Shenkman, Louis; Burday, S. Zane; Blum, Manfred

doi:10.1016/s0140-6736(72)90408-4

Cited by 81 publications

(22 citation statements)

References 19 publications

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“…Six patients with T3 toxicosis, two men and four women, ranging in age from 28 to 43 yr, were studied. Like the other patients we have previously described with this syndrome, they had typical clinical features of hyperthyroidism associated with elevated serum concentrations of Ts and normal serum T4 levels (8). Five had diffuse toxic goiter; one had an autonomous thyroid adenoma.…”

Section: Methodsmentioning

confidence: 53%

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Shenkman¹,

Mitsuma²,

Hollander³

1973

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 53%

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Shenkman¹,

Mitsuma²,

Hollander³

1973

J. Clin. Invest.

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“…As shown in Table I, the ingestion of triiodothyronine in a dose of 25 ug 4 times/d for 7 d resulted in elevation of the serum triiodothyronine concentration from a mean (±SE) control value of 145± 10 to 310±20 ng/dl (P < 0.001). The latter corresponds to serum triiodothyronine levels that occur commonly in patients with spontaneous thyrotoxicosis (37)(38)(39). Serum thyroxine concentrations declined appropriately.…”

Section: Resultsmentioning

confidence: 98%

Triiodothyronine-induced Thyrotoxicosis Increases Mononuclear Leukocyte β-Adrenergic Receptor Density in Man

Ginsberg¹,

Clutter²,

Shah³

et al. 1981

J. Clin. Invest.

108

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A B S T R A C T 3-Adrenergic receptors are increased in some tissues of experimentally thyrotoxic animals but are reported to be unchanged in mononuclear leukocytes of spontaneously thyrotoxic humans. We examined the effects of triiodothyronine (100 ,Lg/d for 7 d) and placebo on high-affinity mononuclear leukocyte P-adrenergic receptors in 24 normal human subjects, using a double-blind design. 3-Adrenergic receptors were assessed by specific binding of the antagonist (-)

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“…The precise concentrations of free T3 and T4 in vivo have not been determined. Estimations by equilibrium dialysis of the percent of total hormone free at physiologic concentrations are about 0.03% for T4 and 0.2-0.4% for T3 (17,21).…”

Section: Resultsmentioning

confidence: 99%

Thyroid Hormone Action in Cell Culture: Demonstration of Nuclear Receptors in Intact Cells and Isolated Nuclei

Samuels

Tsai

1973

Proc. Natl. Acad. Sci. U.S.A.

304

103

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Triiodothyronine and thyroxine induce a 3-fold increase in the rate of growth of GH, cells in culture.To study further the action of these hormones, we examined the binding of [1211] (Grand Island Biological Co., Grand Island, N.Y.). The hormone concentrations in these sera are physiologic and are about 2 nM for T3 and 100 nM for T4, as determined by gas-liquid chromatography and specific radioimmunoassay (5). For hormone-binding studies, the medium was replaced with hypothyroid medium (Ham's F-10 medium supplemented to 10%o with hypothyroid calf serum obtained from a thyroidectomized calf, Rockland Farms, Pa.) and the cultures were incubated for 24-48 hr to deplete the cells of hormone. The hormone concentrations in the thyroidectomized calf serum are 0.3 nM for T3 and 3 nM for T4 (5, 7).Binding Studies with Intact Cells. For binding studies, the cells were harvested in the late logarithmic phase of growth with a rubber policeman and centrifuged at 500 X g for 5 min. The cell pellet was washed three times with 10 ml of serum-free Ham's F-10 medium by repeated dispersion and centrifugation. The cells were then suspended in serum-free medium with various concentrations of [125IVT3 or [125I]T4 and incubated at 370 for the times indicated. All radioactive analysis was done with a gamma spectrometer. After incubation with hormone, the cell suspensions were centrifuged at 500 X g for 5 min and the medium supernatant was saved for determination of hormone concentration. All further procedures were done at 0°. The cell pellet was homogenized in 10 volumes of STM buffer (0.25 M sucrose-20 mM Trist HCl-1.1 mM MgCl2, at pH 7.85 at 250) by 15 strokes at 5000 rpm with a motorized pestle (Tri R, New York). The homogenate was centrifuged at 800 X g for 10 min, and the resultant supernatant was then centrifuged at 16,000 X g -to obtain crude "mitochondrial" and "cytosol" fractions. The homogenate pellet was used to prepare nuclei either by centrifugation at 40,000 X g through 10 ml of 2.2 M sucrose or by two successive suspensions and centrifugations in 10 ml of STM-Triton buffer (0.25 M sucrose-20 mM Tris * HCl-

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Clinical and Laboratory Observations in Cases of Triiodothyronine Toxicosis Confirmed by Radioimmunoassay

Cited by 81 publications

References 19 publications

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Modulation of Pituitary Responsiveness to Throtropin-Releasing Hormone by Triiodothyronine

Triiodothyronine-induced Thyrotoxicosis Increases Mononuclear Leukocyte β-Adrenergic Receptor Density in Man

Thyroid Hormone Action in Cell Culture: Demonstration of Nuclear Receptors in Intact Cells and Isolated Nuclei

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