Clinical and Microbiological Characteristics of a Community-Acquired Carbapenem-Resistant Escherichia coli ST410 Isolate Harbouring blaNDM-5-Encoding IncX3-Type Plasmid From Blood

Gu, Jingjing; Chen, Lin; Weng, Xing‐bei; Yang, Xiaoyan

doi:10.3389/fmed.2021.658058

Cited by 5 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Escherichia coli strains that are ST410 are widely distributed in Europe (Poland, 29 Denmark, 30 Italy, 31 etc. ), Asia (South Korea, 32 Singapore, 33 India, 34 China, 35 etc. ), Africa, 36 North America (United States, 37 Mexico, 38 etc.)…”

Section: Discussionmentioning

confidence: 99%

The First Report of Escherichia coli and Klebsiella pneumoniae Strains That Produce Both NDM-5 and OXA-181 in Jiangsu Province, China

Tao¹,

Tan²,

Chen³

2023

IDR

View full text Add to dashboard Cite

Objective The aim of this study was to analyze the genetic characteristics of three Enterobacteriaceae strains (one strain of Escherichia coli and two strains of Klebsiella pneumoniae ) that produce both the NDM-5 and OXA-181 carbapenemases in pediatric patients. Methods Carbapenem-resistant Enterobacteriaceae (CRE) strains were collected from the Children’s Hospital Affiliated to Nanjing Medical University in 2022. Resistance genes were detected by PCR. CRE strains that produced both the bla NDM-5 and bla OXA-181 genes were further characterized by antimicrobial susceptibility testing, multilocus sequence typing (MLST), plasmid conjugation assay, S1 nuclease-PFGE, Southern blotting and whole-genome sequencing. Results Three Enterobacteriaceae strains carrying both the bla NDM-5 and bla OXA-181 resistance genes were screened. MLST results showed that the strain of Escherichia coli carrying both bla NDM-5 and bla OXA-181 was ST410; the two strains of Klebsiella pneumoniae with both bla NDM-5 and bla OXA-181 were ST2601 and ST759. Conjugation assays showed that the plasmids harboring the bla NDM-5 and bla OXA-181 genes were self-transmissible. S1-PFGE and Southern blotting showed that the bla NDM-5 and bla OXA-181 genes were located on the plasmid with the size of about 60kb~. The genotyping results showed that the plasmid types were ColKP3 and IncX3. Conclusion This is the first report of Enterobacteriaceae strains that produce both NDM-5 and OXA-181 isolated from pediatric patients in China. Active infection control measures are urgently needed to prevent the spread of bacteria in children.

show abstract

Section: Discussionmentioning

confidence: 99%

The First Report of Escherichia coli and Klebsiella pneumoniae Strains That Produce Both NDM-5 and OXA-181 in Jiangsu Province, China

Tao¹,

Tan²,

Chen³

2023

IDR

View full text Add to dashboard Cite

show abstract

“…The carbapenemase resistance gene bla NDM−5 was detected in one of the three carbapenem-resistant strains, and the other two strains were ESBLs-producing. Two carbapenemases, KPC and NDM, were responsible for phenotypic resistance in 90% of the carbapenem-resistant Enterobacteriaceae ( 30 ). Studies unveiled many distinguishing features for their successful persistence and spread ( 31 , 32 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Epidemiology of Escherichia coli From Bloodstream Infection in Shanghai, China, 2016–2019

et al. 2022

View full text Add to dashboard Cite

Background: Bloodstream infections are recognized as important nosocomial infections. Escherichia coli (E. coli) is the most prevalent Gram-negative bacillary pathogen causing bloodstream infections (BSIs). This retrospective study investigated drug susceptibility and molecular epidemiology of E. coli isolated from patients with BSI in Shanghai, China.Methods: We collected E. coli isolated from the blood cultures of patients with BSI between January 2016 and December 2019. We randomly selected 20 strains each year to investigate antimicrobial resistance, resistance genes, and molecular epidemiological characteristics. Antimicrobial susceptibility testing was performed by the disk diffusion method. PCR was performed to detect extended-spectrum β-lactamases (ESBLs), carbapenemase genes, and housekeeping genes, and phyloviz was applied to analyze multilocus sequence typing (MLST).Results: Penicillins, first- and second-generation cephalosporins and fluoroquinolones have high resistance rates (>60%). Among the 80 randomly selected strains, 47 (58.8%) produced ESBLs, and one produced carbapenemase. Sequencing of resistance genes identified blaCTX−M−14 (34%, 16/47), blaCTX−M−15 (23.4%, 11/47) and blaCTX−M−27 (14.8%, 7/47) as the most prevalent genotypes of ESBLs. ST131 (14/80) was the most prevalent sequence type (ST), followed by ST1193 (10/80), ST648 (7/80).Conclusions: Our findings suggest that amikacin, carbapenems, and piperacillin-tazobactam have relatively low resistance rates and may be the preferred antibiotic regimens for empiric therapy. ST131 and blaCTX−M−14 are still the main prevalent in Shanghai with a rapid increase in the occurrence of ST1193 is rapidly increasing and more diverse blaCTX genes.

show abstract

“…15 While Gu's study suggested that CPECO strains in blood cultures might originate from intestinal colonization strains, as they had highly similar PFGE fingerprinting. 16 Previous studies had also shown a higher rate of CRE infection in patients who were CRE intestinal colonization carriers compared to non-CRE colonization patients. 17 We hypothesized that the probable pathway of CPECO infection in patients with biliary system diseases involved the translocation of CPECO strains from the intestinal tract, particularly with the assistance of certain invasive procedures, such as ERCP surgery, which might accelerate the occurrence of translocation and infection.…”

mentioning

confidence: 96%

Clinical and Molecular Characteristics of Carbapenemase-Producing E. coli Strains from Patients with Biliary System Diseases and Hematological Malignancies

Qian,

Bao,

et al. 2023

IDR

View full text Add to dashboard Cite

This study aims to investigate the clinical and molecular characteristics of carbapenemase-producing E. coli strains (CPECO). Patients and Methods: We collected 38 non-repetitive CPECO strains, identified them using MALDI-TOF, and assessed their antimicrobial susceptibility via the VITEK-Compact II system. We gathered demographic and clinical patient data. Phenotypic assays were employed to detect carbapenemase types. Polymerase chain reaction (PCR) was utilized to identify the carbapenemase genes. Seven housekeeping genes were amplified and sequenced to determine the multilocus sequence typings (MLSTs). Results: These CPECO strains, primarily isolated from aseptic site and stool screening specimens, exhibited significant resistance to most clinical antibiotics, except for tigecycline and amikacin. Most patients had underlying medical conditions and underwent invasive procedures. There were significant differences among patients concerning the presence of malignancies, digestive system disorders, endoscopic retrograde cholangiopancreatography (ERCP) surgeries and abdominal drainage tubes. However, no significant differences were observed among patients regarding conditions, including hypertension, diabetes, respiratory diseases, urinary diseases and cardiovascular diseases, as well as invasive procedures such as deep venous catheterization, endotracheal intubation and gastrointestinal catheterization. Metallo-β-lactamase was primarily responsible for carbapenem resistance, including bla NDM-5 (24/38), bla NDM-1 (5/38), bla NDM-9 (1/38) and bla IMP-4 (1/38). Additionally, 7 CPECO strains carried bla KPC-2 . The distribution of CPECO sequence types (STs) was diverse, with seven strains being ST131, six strains being ST410, three strains each of ST1196 and ST10, although most STs were represented by only one strain. Conclusion: CPECO infections in patients with biliary system diseases may result from intestinal CPECO translocation, with ERCP surgery potentially facilitating this. Meanwhile, malignant tumor was found to be a significant factor affecting CPECO infections in patients with hematological diseases. bla NDM-5 , bla NDM-1 and bla NDM-9 were primarily responsible for carbapenem resistance in CPECO strains. The emergence of carbapenem-resistant ST131 and ST410 strains should be alert to prevent the spread of carbapenemresistant genes within high-risk epidemic clones.

show abstract

Clinical and Microbiological Characteristics of a Community-Acquired Carbapenem-Resistant Escherichia coli ST410 Isolate Harbouring blaNDM-5-Encoding IncX3-Type Plasmid From Blood

Cited by 5 publications

References 25 publications

The First Report of Escherichia coli and Klebsiella pneumoniae Strains That Produce Both NDM-5 and OXA-181 in Jiangsu Province, China

The First Report of Escherichia coli and Klebsiella pneumoniae Strains That Produce Both NDM-5 and OXA-181 in Jiangsu Province, China

Antimicrobial Resistance and Molecular Epidemiology of Escherichia coli From Bloodstream Infection in Shanghai, China, 2016–2019

Clinical and Molecular Characteristics of Carbapenemase-Producing E. coli Strains from Patients with Biliary System Diseases and Hematological Malignancies

Contact Info

Product

Resources

About