Clinical and Molecular Characteristics of Human Rotavirus G8P[8] Outbreak Strain, Japan, 2014

Kondo, Kenji; Tsugawa, Takeshi; Ono, Masaji; Ohara, Taku; Fujibayashi, Shinsuke; Tahara, Yasuo; Kubo, Nobuhiko; Nakata, Shuji; Higashidate, Yoshihito; Fujii, Yoshiki; Katayama, Kazuhiko; Yoto, Yuko; Tsutsumi, Hiroyuki

doi:10.3201/eid2306.160038

Cited by 44 publications

(48 citation statements)

References 15 publications

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“…The frequency of untypable G or P‐genotype specimens was 13.07% and 8.66%, respectively. On one hand, it may be the result of a mismatch between primers and target sequences, as new G and P genotypes appeared in recent years . Some mismatches were observed after alignment of the primers and the VP7 and VP4 sequences of recently appeared genotypes.…”

Section: Discussionmentioning

confidence: 99%

“…On one hand, it may be the result of a mismatch between primers and target sequences, as new G and P genotypes appeared in recent years. [41][42][43][44][45] Some mismatches were observed after alignment of the primers and the VP7 and VP4 sequences of recently appeared genotypes. The corresponding sequences of these untypable rotavirus strains can be obtained by optimizing primer sequences or by Sequence-Independent-Single-Primer-Amplification for genotyping in the future.…”

Section: Phylogenetic Analysis Included the Vp7 And Vp4 Genes Of G9mentioning

confidence: 99%

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Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Zeng

Zhao

et al. 2019

Journal of Medical Virology

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Human group A rotavirus (RVA) is the leading cause of acute viral gastroenteritis in children under 5 years old worldwide. The aim of this study was to investigate the genotype distribution of RVA in the Midwest of China. Sentinel‐based surveillance of acute diarrhea was conducted at Children's Hospital of Chongqing Medical University from 2011 to 2015. RVA was tested by using enzyme‐linked immunosorbent assays. The partial VP4 genes and VP7 genes of rotavirus were amplified and sequenced, and genotyping and phylogenetic analyses were performed. Among the 2236 stool specimens collected from children with acute gastroenteritis, 681 (30.46%) were positive for RVA. The majority of children (89.28%) who tested positive for RVA were children aged ≤2 years. The seasonal peak of RVA was in the winter. As for genotype, four strain combinations, G9P[8], G3P[8], G1P[8], and G2P[4] contributed to 75.62% (515/681) of the RVA‐associated diarrhea cases. After a marked increase in G9P[8] (30.77%) in 2013, G9P[8] became the predominant genotype in 2014 and 2015, whilst the prevalence of G1P[8] was decreased to 2.72% in 2015. Unusual G‐P combinations (eg, G1P[4], G9P[4], G4P[6], G3P[4], G2P[8]) were also detected sporadically over the study period. Phylogenetic tree analysis results showed that the VP7 sequences of G9 strains were clustered into two main lineages, and 77.34% of them were clustered into lineage VI, with the highest nucleotide similarity to the strain JS12‐17(China). VP4 gene sequences of P[8] strains were almost P[8]‐lineage 3. Substantial temporal variation in the circulation of various genotypes of rotavirus in Chongqing was observed during 2011‐2015, and highlights the need for continuous surveillance of RVA infection for better understanding and control of RVA infection.

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Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Analysis Included the Vp7 And Vp4 Genes Of G9mentioning

confidence: 99%

Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Zeng

Zhao

et al. 2019

Journal of Medical Virology

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“…Furthermore, in Tomakomai, close to Sapporo in Hokkaido, a G8P [8]I2 epidemic was seen in 2014, although just one G8P [8]I2 strain was detected in Sapporo that year. 25 Because the epidemic RV GPI genotype in each city is not always the same, surveillance at the city level is necessary to obtain an accurate genotype distribution for that area.…”

Section: Discussionmentioning

confidence: 99%

Rotavirus genotype and Vesikari score of outpatients in Japan in the vaccine era

Ono

Tsugawa

Nakata³

et al. 2020

Pediatrics International

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Background: Group A rotaviruses (RVs) are a major cause of severe gastroenteritis among infants and young children. In Japan, RV vaccines were introduced in 2011, leading to a reduction in severe gastroenteritis cases. Studies are required to assess the effectiveness of the vaccines and their effect on the prevalence of RV genotypes. Methods: Fecal samples were collected from outpatients with RV gastroenteritis in a pediatric clinic in Sapporo, Japan, from 2010 to 2016. GPI genotypes were determined using reverse-transcription polymerase chain reaction. Clinical information and immunization records were obtained from outpatients after 2013. GPI genotypes and clinical features were compared between patients with and without a RV vaccine history. Results: In total, 270 cases were genotyped. G1P[8]I1 (Wa-like G1P[8]) strains were dominant from 2010 to 2012. G1P[8]I2 (DS-1-like G1P[8]) strains appeared in 2012 and dominated in 2013 to 2015. G2P[4]I2 and G9P[8]I1 strains increased every 3 years (G2P[4]I2: 2011 and 2014, G9P[8] I1: 2010, 2013 and 2016). After the 2013 season, 137 cases were collected, 24 of which were vaccinated. Cases requiring drip infusion were fewer in the vaccination group than in the non-vaccination group (16.7% vs 52.2%). No patients required hospitalization in the vaccination group compared with 10.6% in the non-vaccination group. A severe Vesikari score was less common in the vaccination group than in the non-vaccination group (33.3% vs 78.8%). There was no significant difference in the GPI genotype distribution between the two groups. Conclusion: Rotaviruses vaccine effectiveness, regardless of GPI genotype, was confirmed in terms of alleviation of disease severity.

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“…On phylogenetic analysis, strains DBM2017-016 and DBM2018-291 formed a cluster with these co-circulating Wa-like G9P [8] human strains within human-like G9 lineage-3, in which the majority of globally circulating G9 HuRVA strains cluster (Fig 2A). On the other hand, the VP7 gene of strain DBM2017-203 exhibited the highest nucleotide sequence similarity (99.8%) with contemporary French Wa-like G9P [8] human strain Dijon-R10484 [42] (Table 1 and Fig 1), and somewhat lower identities (99.4 and 99.1%, respectively) with Italy Wa-like G9P [8] human strain JES11 [43] and Japanese Wa-like G9P [8] strain UR14-16 [23]. Phylogenetically, strain DBM2017-203 was very closely related with strain Dijon-R10484 in a common branch with [8] strains (DBM2017-016, DBM2017-203, and DBM2018-291) are shown in red, while the DS-1-like G1P [8] strains and their derivatives that have been reported are shown in blue.…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

“…In 2013, DS-1-like (equine-like) G3P [8] strains emerged in Thailand and Australia [12,22], and equine-like G3P [8] strains have successfully spread to several parts of the world, i.e., Dominica in 2014 [18], Germany, Hungary, Indonesia, Japan, Spain, and the United States in 2015 [6,10,13,20,28,29,32], Brazil in 2016 [16], and Italy in 2018 [14]. In 2013, DS-1-like (bovine-like) G8P [8] strains also emerged in Thailand [31], and subsequently bovine-like G8P [8] strains were detected in the Czech Republic, Japan, and Vietnam in 2014 [17,23,26], and Singapore in 2016 [11].…”

Section: Introductionmentioning

confidence: 99%

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

et al. 2020

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The emergence and rapid spread of unusual DS-1-like intergenogroup reassortant rotaviruses having G1/3/8 genotypes have been recently reported from major parts of the world (Africa, Asia, Australia, Europe, and the Americas). During rotavirus surveillance in Thailand, three novel intergenogroup reassortant strains possessing the G9P[8] genotype (DBM2017-016, DBM2017-203, and DBM2018-291) were identified in three stool specimens from diarrheic children. In the present study, we determined and analyzed the full genomes of these three strains. On full-genomic analysis, all three strains were found to share a unique genotype constellation comprising both genogroup 1 and 2 genes: G9-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis demonstrated that each of the 11 genes of the three strains was closely related to that of emerging DS-1-like intergenogroup reassortant, human, and/or locally circulating human strains. Thus, the three strains were suggested to be multiple reassortants that had acquired the G9-VP7 genes from co-circulating Wa-like G9P[8] rotaviruses in the genetic background of DS-1-like intergenogroup reassortant (likely equine-like G3P[8]) strains. To our knowledge, this is the first description of emerging DS-1-like intergenogroup reassortant strains having the G9P[8] genotype. Our observations will add to the growing insights into the dynamic evolution of emerging DS-1like intergenogroup reassortant rotaviruses through reassortment.

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Clinical and Molecular Characteristics of Human Rotavirus G8P[8] Outbreak Strain, Japan, 2014

Cited by 44 publications

References 15 publications

Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Rotavirus genotype and Vesikari score of outpatients in Japan in the vaccine era

Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

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