Clinical and Prognostic Significance of SOX11 in Breast Cancer

Liu, Dao-Tong; PengZhao,; Han, Jing‐Yan; Lin, Fanzhong; Bu, Xianmin; Xu, Qianqian

doi:10.7314/apjcp.2014.15.13.5483

Cited by 15 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…demonstrated that expression of SOX11 in BC tissues was significantly higher than in normal tissue, in the Chinese. This overexpression is correlated with smaller tumor size and milder tumor grade, indicating that SOX11 could inhibit growth and progression of BC, and promote absence of lymph node metastasis35. Both LEP and ADIPOQ are adipokines secreted by adipocytes.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of breast cancer in Lebanese women

Makoukji

Makhoul

Khalil

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER®/Pathway Studio®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of breast cancer in Lebanese women

Makoukji

Makhoul

Khalil

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The transcription factor SOX11, potentially of prognostic value but not initially identified in the STRING analysis, has previously been shown to have a role in breast cancer growth and invasion, and in regulating the basal-like phenotype (48). SOX11, described by others as a marker of poor prognosis in basal-like breast cancer (48)—although another study unstratified for subtype did not show this effect (49)—has been proposed as a therapeutic target in breast (48) and other (50) cancers. As noted above, SOX11 can be included in the STRING network through its interaction, identified by coimmunoprecipitation and LC-MS/MS (19) with the purine biosynthetic protein GART.…”

Section: Discussionmentioning

confidence: 99%

Novel Prognostic Markers in Triple-Negative Breast Cancer Discovered by MALDI-Mass Spectrometry Imaging

2019

View full text Add to dashboard Cite

There are no widely-accepted prognostic markers currently available to predict outcomes in patients with triple-negative breast cancer (TNBC), and no targeted therapies with confirmed benefit. We have used MALDI mass spectrometry imaging (MSI) of tryptic peptides to compare regions of cancer and benign tissue in 10 formalin-fixed, paraffin-embedded sections of TNBC tumors. Proteins were identified by reference to a peptide library constructed by LC-MALDI-MS/MS analyses of the same tissues. The prognostic significance of proteins that distinguished between cancer and benign regions was estimated by Kaplan-Meier analysis of their gene expression from public databases. Among peptides that distinguished between cancer and benign tissue in at least 3 tissues with a ROC area under the curve >0.7, 14 represented proteins identified from the reference library, including proteins not previously associated with breast cancer. Initial network analysis using the STRING database showed no obvious functional relationships except among collagen subunits COL1A1, COL1A2, and COL63A, but manual curation, including the addition of EGFR to the analysis, revealed a unique network connecting 10 of the 14 proteins. Kaplan-Meier survival analysis to examine the relationship between tumor expression of genes encoding the 14 proteins, and recurrence-free survival (RFS) in patients with basal-like TNBC showed that, compared to low expression, high expression of nine of the genes was associated with significantly worse RFS, most with hazard ratios >2. In contrast, in estrogen receptor-positive tumors, high expression of these genes showed only low, or no, association with worse RFS. These proteins are proposed as putative markers of RFS in TNBC, and some may also be considered as possible targets for future therapies.

show abstract

“…These findings demonstrate that the roles of SOX11 are likely to be dependent on the context of the tumor and cell type. In breast cancers, one group has recently shown that high grade breast tumors had lower levels of nuclear SOX11 protein, and that nuclear SOX11 was associated with improved clinical outcome [ 44 ]. Our results reported here combined with these previous studies support further evaluation of the role and clinical significance of SOX11.…”

Section: Discussionmentioning

confidence: 99%

The SOX11 transcription factor is a critical regulator of basal-like breast cancer growth, invasion, and basal-like gene expression

et al. 2016

View full text Add to dashboard Cite

Basal-like breast cancers (BLBCs) are aggressive breast cancers associated with poor survival. Defining the key drivers of BLBC growth will allow identification of molecules for targeted therapy. In this study, we performed a primary screen integrating multiple assays that compare transcription factor expression and activity in BLBC and non-BLBC at the RNA, DNA, and protein levels. This integrated screen identified 33 transcription factors that were elevated in BLBC in multiple assays comparing mRNA expression, DNA cis-element sequences, or protein DNA-binding activity. In a secondary screen to identify transcription factors critical for BLBC cell growth, 8 of the 33 candidate transcription factors (TFs) were found to be necessary for growth in at least two of three BLBC cell lines. Of these 8 transcription factors, SOX11 was the only transcription factor required for BLBC growth, but not for growth of non-BLBC cells. Our studies demonstrate that SOX11 is a critical regulator of multiple BLBC phenotypes, including growth, migration, invasion, and expression of signature BLBC genes. High SOX11 expression was also found to be an independent prognostic indicator of poor survival in women with breast cancer. These results identify SOX11 as a potential target for the treatment of BLBC, the most aggressive form of breast cancer.

show abstract

Clinical and Prognostic Significance of SOX11 in Breast Cancer

Cited by 15 publications

References 21 publications

Gene expression profiling of breast cancer in Lebanese women

Gene expression profiling of breast cancer in Lebanese women

Novel Prognostic Markers in Triple-Negative Breast Cancer Discovered by MALDI-Mass Spectrometry Imaging

The SOX11 transcription factor is a critical regulator of basal-like breast cancer growth, invasion, and basal-like gene expression

Contact Info

Product

Resources

About