Clinical and prognostic value of hTERT mRNA expression in patients with non-small-cell lung cancer

Zalewska‐Ziob, Marzena; Dobija-Kubica, Katarzyna; Biernacki, Krzysztof; Adamek, Brygida; Kasperczyk, Janusz; Bruliński, Krzysztof; Ostrowska, Zofia

doi:10.18388/abp.2017_1618

Cited by 9 publications

(10 citation statements)

References 23 publications

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“…An increasing amount of research has also focused on the relationship between the hTERT and non-small cell lung cancer. It has been reported that repression of hTERT promoted the prognosis of early stage non-small cell lung cancer [ 32 ]. However, understanding of the mechanism underlying hTERT involvement in the regulation of SCLC remains unclear to the best of our knowledge [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

hTERT promoter methylation promotes small cell lung cancer progression and radiotherapy resistance

Zhai

Zheng

et al. 2020

Journal of Radiation Research

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Small cell lung cancer (SCLC) has been a devastating actuality in clinic and the molecular mechanisms underlying this disease remain unclear. The epigenetic alterations located in the promoter region of human telomerase reverse transcriptase (hTERT) have been demonstrated as one of the most prevalent non-coding genomic modifications in multiple cancers. However, alteration of hTERT promoter methylation in SCLC and the subsequently induced change in tumor cell behavior remains unclear. In this research, we hypothesized that abnormal methylation of hTERT promotor enhanced the progression of SCLC and the outcome of radiotherapy resistance. Quantitative real-time PCR and western blot assays were performed to evaluate the RNA and protein levels of hTERT and enhancer of zeste homolog 2 (EZH2), respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to estimate the viability and X-ray sensitivity of H20 and H446 cell lines. Functionally, upregulation of hTERT promoted the proliferation and migration of H20 and H446 cells, and the high-level of methylation in the promoter region of hTERT induced by radiation caused radio-resistance in SCLC. Mechanically, methylation of hTERT promoter enhanced the progression and radio-resistance of SCLC through upregulating the expression of its downstream effector EZH2.

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Section: Discussionmentioning

confidence: 99%

hTERT promoter methylation promotes small cell lung cancer progression and radiotherapy resistance

Zhai

Zheng

et al. 2020

Journal of Radiation Research

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“…Most of the tumor tissues have been identified by hTERT expression, and more than 85% of cancer cells contain genetic aberrations and hTERT overexpression ( 13 ). In this study, our data analysis led us to believe that hTERT is also increased in the patients with ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

ShRNA-mediated silencing of hTERT promote apoptosis and senescence in human ovarian cancer cells

Chen¹,

Luo²,

Yang³

et al. 2019

Transl. Cancer Res

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Background Human telomerase reverse transcriptase (hTERT) is a crucial oncogene and a key factor in cell immortalization. Apart from hematopoiesis stem cell and germ cells, normal cells and tissues almost no expression of this gene. However, the positive expression rate of hTERT in malignant tumors is as high as 80–95%. This study was designed to investigate the mechanism of knockdown of hTERT expression in the regulation of ovarian cancer carcinogenesis in nude mice transplantation model. Methods We identified the function of hTERT in the treatment of ovarian cancer by constructing a nude mice transplantation tumor model. Then analyzed the mechanism of hTERT in modulating ovarian cancer cell apoptosis and senescence by immunohistochemically staining. Results Our analysis identified hTERT as a novel regulator of ovarian cancer progression and is one of the most significantly upregulated genes in ovarian cancer. Down-regulation of hTERT inhibits proliferation of ovarian cancer cells by promoting apoptosis and the expression of the senescence gene p53 and p21 . Conclusions hTERT is a key oncogene in ovarian tumorigenesis and metastasis; the downregulation of hTERT significantly inhibit the proliferation of ovarian cancer and have an effect on treatment of ovarian cancer by activating cell apoptosis and senescence pathway.

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“…Moreover, MYC inhibition induced tumor regression in K-Ras-driven LC in mice as well as in p53-deficient animals [121], which suggests the contribution of MYC for the progression of these tumors. hTERT expression has been reported (33% to 94%) in NSCLCs with poorer overall survival at three years [122] and it was significantly higher in NSCLC when compared with non-tumor tissues [123]. Among NSCLCs, AdCs display an increased expression of hTERT when compared with SQCs [122].…”

Section: Pathogenesis Of Lung Cancermentioning

confidence: 97%

Epstein–Barr Virus Infection in Lung Cancer: Insights and Perspectives

et al. 2022

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Lung cancer (LC) is the leading cause of cancer death worldwide. Tobacco smoke is the most frequent risk factor etiologically associated with LC, although exposures to other environmental factors such as arsenic, radon or asbestos are also involved. Additionally, the involvement of some viral infections such as high-risk human papillomaviruses (HR-HPVs), Merkel cell polyomavirus (MCPyV), Jaagsiekte Sheep Retrovirus (JSRV), John Cunningham Virus (JCV), and Epstein–Barr virus (EBV) has been suggested in LC, though an etiological relationship has not yet been established. EBV is a ubiquitous gamma herpesvirus causing persistent infections and some lymphoid and epithelial tumors. Since EBV is heterogeneously detected in LCs from different parts of the world, in this review we address the epidemiological and experimental evidence of a potential role of EBV. Considering this evidence, we propose mechanisms potentially involved in EBV-associated lung carcinogenesis. Additional studies are warranted to dissect the role of EBV in this very frequent malignancy.

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Clinical and prognostic value of hTERT mRNA expression in patients with non-small-cell lung cancer

Cited by 9 publications

References 23 publications

hTERT promoter methylation promotes small cell lung cancer progression and radiotherapy resistance

hTERT promoter methylation promotes small cell lung cancer progression and radiotherapy resistance

ShRNA-mediated silencing of hTERT promote apoptosis and senescence in human ovarian cancer cells

Epstein–Barr Virus Infection in Lung Cancer: Insights and Perspectives

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