Clinical and Virological Characteristics of Hepatitis B Virus Subgenotypes Ba, C1, and C2 in China

Wang, Zhanhui; Tanaka, Yasuhito; Huang, Yuehua; Kurbanov, Fuat; Chen, Jinjun; Zeng, G.; Zhou, Bin; Mizokami, Masashi; Hou, Jinlin

doi:10.1128/jcm.02157-06

Cited by 73 publications

(84 citation statements)

References 37 publications

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“…For instance, the frequency of double mutation (A1762T/G1764A) increased with advancing clinical status in Taiwanese patients [3%, 11%, 32% and 64% in asymptomatic carriers (AC), LC, CH, and HCC groups, respectively] [24] . A recent report from China has also demonstrated that the incidence of double mutation increased along with the progression of liver disease; the percentage of the double mutation was 33%, 56% and 85% in CH, LC, and HCC groups, respectively [31] . In Indonesian patients, however, the A1762T/G1764A double mutation was increased in CH from 19.7% to 59.7% in LC and was slightly decreased in HCC (54.2%) ( Table 4).…”

Section: Discussionmentioning

confidence: 99%

“…The predominant mutation in the precore region of HBV which involved a G-to-A change at nucleotide 1896, and resulted in a premature stop codon at codon 28, was proved to be associated with increased HCC risk [23,[28][29][30] . In addition, among HBV carriers, the A1762T/G1764A mutation is more frequently found in genotype C than genotype B [19,31] . However, an independent study on a comparison of HBV genotype C from Vietnam and Japan showed mutations at different positions in the core promoter/precore region of HBV [32] , indicating that the effect of mutation on liver carcinogenesis may not be universal.…”

Section: Introductionmentioning

confidence: 99%

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Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

Utama¹,

Purwantomo

Siburian

et al. 2009

WJG

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AIM:To identify the distribution of hepatitis B virus (HBV) subgenotype and basal core promoter (BCP) mutations among patients with HBV-associated liver disease in Indonesia. METHODS:Patients with chronic hepatitis (CH, n = 61), liver cirrhosis (LC, n = 62), and hepatocellular carcinoma (HCC, n = 48) were included in this study.HBV subgenotype was identified based on S or preS gene sequence, and mutations in the HBx gene including the overlapping BCP region were examined by direct sequencing. RESULTS:HBV genotype B (subgenotypes B2, B3, B4, B5 and B7) the major genotype in the samples, accounted for 75.4%, 71.0% and 75.0% of CH, LC and HCC patients, respectively, while the genotype C (subgenotypes C1, C2 and C3) was detected in 24.6%, 29.0%, and 25.0% of CH, LC, and HCC patients, respectively. Subgenotypes B3 (84.9%) and C1 (82.2%) were the main subgenotype in HBV genotype B and C, respectively. Serotype adw2 (84.9%) and adrq+ (89.4%) were the most prevalent in HBV genotype B and C, respectively. Double mutation (A1762T/G1764A) in the BCP was significantly higher in LC (59.7%) and HCC (54.2%) than in CH (19.7%), suggesting that this mutation was associated with severity of liver disease. The T1753V was also higher in LC (46.8%), but lower in HCC (22.9%) and CH (18.0%), suggesting that this mutation may be an indicator of cirrhosis. CONCLUSION:HBV genotype B/B3 and C/C1 are the major genotypes in Indonesia. Mutations in BCP, such as A1762T/G1764A and T1753V, might have an association with manifestations of liver disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

Utama¹,

Purwantomo

Siburian

et al. 2009

WJG

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“…Similarly, a cross-sectional study of 211 patients with various stages of liver disease in China showed the distribution of HBV genotype C was greater among cirrhosis and HCC patients, while genotype B was common in chronic hepatitis patients. In addition, no significant differences in clinical features were found between patients with HBV subgenotypes B2, C1, and C2 [68]. Taken together, further elaborated studies are needed to examine the clinical impact of each HBV subgenotype on the pathogenesis and progression of liver diseases.…”

Section: Genotype and Subgenotypementioning

confidence: 96%

Role of viral factors in the natural course and therapy of chronic hepatitis B

Kao

2007

Hepatol Int

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Hepatitis B virus (HBV) infection is a global health problem that causes a wide spectrum of liver disease, including acute or fulminant hepatitis, inactive carrier state, chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). The pathogenesis of hepatocyte damage associated with HBV is mainly through immune-mediated mechanisms. On the basis of the virus and host interactions, the natural history of HBV carriers who are infected in early life can be divided into four dynamic phases. The frequency, extent, and severity of hepatitis flares or acute exacerbation in the second immune clearance and/or fourth reactivation phase predict liver disease progression in HBV carriers. In the past decade, hepatitis B viral factors including serum HBV DNA level, genotype, and naturally occurring mutants predictive of clinical outcomes have been identified. The higher the serum HBV DNA level after the immune clearance phase, the higher the incidence of adverse outcomes over time. In addition, high viral load, genotype C, basal core promoter mutation, and pre-S deletion correlate with increased risk of cirrhosis and HCC development. As to the treatment of chronic hepatitis B, patients with high HBV DNA level and genotype C or D infection are shown to have a worse response to interferon therapy. In conclusion, serum HBV DNA level, genotype, and naturally occurring mutants are identified to influence liver disease progression and therapy of chronic hepatitis B. More investigations are needed to clarify the molecular mechanisms of the viral factors involved in the pathogenesis of each stage of liver disease and the response to antiviral treatments.

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“…The G1896A mutation is supposed to increase the stability of the stem-loop structure. Recent reports suggest that HBV subgenotypes Ba, C1, and C2 have an intermediate frequency of 1896A mutation [43] . Interestingly, although the son was infected via the mother, none of the BCP and 1896A mutants were found in the son (0/9).…”

Section: Cacatttcctgmentioning

confidence: 99%

Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission

Shen¹,

Yan²,

Zou³

et al. 2008

WJG

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AIM:To determine whether HBV with the same characteristics causes dissimilar mutations in different hosts. METHODS: Full-length HBV genome was amplified and linked with pMD T18 vector. Positive clones were selected by double-restriction endonuclease digestion (Eco RⅠ and Hin dⅢ) and PCR. Twenty seven clones were randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and 6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis was performed using BioEditor, Clustal X and MEGA software. Potential immune epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method and Emini Surface Accessibility Prediction. RESULTS: All of the 27 sequences were genotype C, the divergence between the mother and son was 0%-0.8%. Compared with another 50 complete sequences of genotype C, the mother and her son each had 13 specific nucleotides that differed from the other genotype C isolates. AA 1-11 deletion in preS1 was the dominant mutation in the mother (14/18). The 1762T/1764A double mutation existed in all clones of the mother, 3 of them were also coupled with G1896A mutation, but none were found in the son. 17 bp deletion starting at nucleotide 2330 was the major mutation (5/9) in the son, which caused seven potential HLA class Ⅰ epitopes and one B cell epitope deletion, and produced a presumptive new start codon, downstream from the original one of the P gene. CONCLUSION: The HBV strain in the son came from his mother, and discrepant mutation occurred in the mother and her son during infection.

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Clinical and Virological Characteristics of Hepatitis B Virus Subgenotypes Ba, C1, and C2 in China

Cited by 73 publications

References 37 publications

Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

Role of viral factors in the natural course and therapy of chronic hepatitis B

Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission

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