Clinical Applicability of in vivo Reflectance Confocal Microscopy for the Diagnosis of Actinic Keratoses

Ulrich, Martina; Maltusch, Adrienne; Rius-Díaz, Francisca; Röwert‐Huber, Joachim; González, Salvador; Sterry, Wolfram; Stockfleth, Eggert; Astner, Susanne

doi:10.1097/00042728-200805000-00003

Cited by 61 publications

(123 citation statements)

References 24 publications

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“…Later, others confirmed the usefulness of this tool [73,[88][89], although it has not been able to unequivocally differentiate actinic keratosis from squamous cell carcinomas [90]. Non-melanoma skin cancers (NMSC) were evaluated by fluorescence confocal microscopy aiming to diagnose and monitor the lesions in reference to normal skin and correlation with routine histology.…”

Section: Crm and Non-melanocitic Lesionsmentioning

confidence: 99%

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

Rossetti¹,

Depieri²,

Bentley³

2013

Confocal Laser Microscopy - Principles and Applications in Medicine, Biology, and the Food Sciences

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Section: Crm and Non-melanocitic Lesionsmentioning

confidence: 99%

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

Rossetti¹,

Depieri²,

Bentley³

2013

Confocal Laser Microscopy - Principles and Applications in Medicine, Biology, and the Food Sciences

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“…One of the most informative is in vivo reflectance confocal microscopy (RCM), which provides real-time optical transversal sections 1 of the skin area of interest, giving diagnostic information that, in some cases, parallels that of histology and has been recently summarized in these reviews. 2,3 The transversal sections can be acquired at sequential depths with an axial resolution of 3 to 5 μm and arranged in image stacks that document the three-dimensional skin architecture. 4 In most cases, analysis of the image stacks or individual optical slices is done manually.…”

Section: Introductionmentioning

confidence: 99%

Geometrical and topological analysis ofin vivoconfocal microscopy images reveals dynamic maturation of epidermal structures during the first years of life

et al. 2015

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Abstract. Reflectance confocal microscopy is successfully used in infant skin research. Infant skin structure, function, and composition are undergoing a maturation process. We aimed to uncover how the epidermal architecture and cellular topology change with time. Images were collected from three age groups of healthy infants between one and four years of age and adults. Cell centers were manually identified on the images at the stratum granulosum (SG) and stratum spinosum (SS) levels. Voronoi diagrams were used to calculate geometrical and topological parameters. Infant cell density is higher than that of adults and decreases with age. Projected cell area, cell perimeter, and average distance to the nearest neighbors increase with age but do so distinctly between the two layers. Structural entropy is different between the two strata, but remains constant with time. For all ages and layers, the distribution of the number of nearest neighbors is typical of a cooperator network architecture. The topological analysis provides evidence of the maturation process in infant skin. The differences between infant and adult are more pronounced in the SG than SS, while cell cooperation is evident in all cases of healthy skin examined.

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“…noma skin cancer (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). It is also used for in vivo and ex vivo evaluation of nondermatological diseases such as neoplasia of mucosal tissues from mouth and cervix, mammary glands and neural tissue (11)(12)(13)(14).…”

mentioning

confidence: 99%

Cellular features of psoriatic skin: Imaging and quantification using in vivo reflectance confocal microscopy

Wolberink

Erp

Teussink

et al. 2010

Cytometry Part B Clinical

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Background: In vivo reflectance confocal microscopy (RCM) is a novel, exciting imaging technique. It provides images of cell-and tissue structures and dynamics in situ, in real time, without the need for ex vivo tissue samples. RCM visualizes the superficial part of human skin up to a depth of 250 lm. In psoriasis, an erythematosquamous skin disease, we evaluated well known histological features of stable psoriasis vulgaris (PP) with RCM. RCM images were correlated to morphological and cell biological findings in routine HE and immunohistochemical stained histology with CD3 and antifilaggrin antibodies.Methods: Lesional and nonlesional skin of eight patients with PP were evaluated with RCM, after which 4-mm punch biopsies were taken and cut vertically in two equal parts. One part was processed in the conventional vertical way, the other horizontally (en face) for optimal correlation to RCM images. We evaluated and quantificated nine histopathological features of psoriasis: parakeratosis, epidermal and dermal inflammatory infiltrate, diminished or absent stratum granulosum, epidermal thickening, thinning of the suprapapillary epidermal plate, increased height of the papillary dermis, increase in number of dermal papillae and increase in number and volume of papillary capillaries.Results: Quantification and evaluation of cell biological and histological features of PP with RCM correlated highly to evaluation in HE, CD3 and filaggrin-stained histology.Conclusions: RCM is a novel technique which can be used for real time, cytometric evaluation and quantification of PP features. RCM might be suited equally for cytometric evaluation of other superficial tissues.

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Clinical Applicability of in vivo Reflectance Confocal Microscopy for the Diagnosis of Actinic Keratoses

Cited by 61 publications

References 24 publications

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

Confocal Laser Scanning Microscopy as a Tool for the Investigation of Skin Drug Delivery Systems and Diagnosis of Skin Disorders

Geometrical and topological analysis ofin vivoconfocal microscopy images reveals dynamic maturation of epidermal structures during the first years of life

Cellular features of psoriatic skin: Imaging and quantification using in vivo reflectance confocal microscopy

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