Several treatment modalities have been applied over the years to achieve periodontal regeneration with no predictable outcome. However, in the emerging field of regenerative medicine, the mesenchymal stem cells derived from human fetal membrane have shown promising results. The amniotic sac encloses the developing foetus through gestation and is composed of amnion and chorion membranes. The clinical application of these membranes not only maintains the structural and anatomical configuration of regenerated tissues, but also contributes to the enhancement of healing through reduction of post-operative scarring and providing a rich source of stem cells. Periodontal regeneration using amniotic membrane provides excellent predictability, improved long-term outcomes, and superior aesthetics over other treatment options.