Clinical application of LC-MS/MS to detect fat-soluble vitamins in gastric cancer

Abstract

Purpose Gastric cancer is one of the most common malignant tumors, with the incidence and mortality ranking among the top four malignant tumors in the world. In recent years, more and more studies have reported that fat-soluble vitamins are involved in tumor metabolism. However, the sensitivity and specificity of conventional detection methods are low, while liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has the advantages of high sensitivity, high specificity, high stability and wide linear range, which makes up for the shortcomings of immunological methods. The purpose of this study was to accurately detect the content of fat-soluble vitamins in GC patients by LC-MS/MS, and to clarify the necessity of preoperative determination and regular postoperative monitoring. Methods Serum 25(OH)D3, 25(OH)D2, VA, VE and VK1 levels in gastric cancer patients and healthy volunteers were detected by LC-MS/MS. Results The results showed that the levels of serum 25(OH)D3, 25(OH)D2, VA and VK1 in gastric cancer patients were significantly lower than those in healthy volunteers, and there was no significant difference in VE content. Conclusion This study suggests that patients with gastric cancer should timely detect and supplement fat-soluble vitamins and LC-MS/MS will provide a more accurate detection protocol for the clinic.