Clinical Application of Simplified Serum Prothrombin Consumption Test

Abstract

The serum prothrombin consumption test, described by Quick,1 has not been commonly used because of its complicated technique. A simplified method using commercially prepared reagents, described by Wald, Weiner, and Sussman,2 is now available. The test can easily be performed in any laboratory where plasma prothrombin determinations are done. No single explanation for the coagulation of blood has as yet been generally accepted, nor has unanimity on the matter of terminology been reached; however, practically all of the recently expounded theories by Seeger, Quick, Owren, and Ware are schematically represented in figure 1.The plasma prothrombin time measures deficiencies of the prothrombin complex. Thus a reduction in prothrombin, either congenital or acquired by the administration of bishydroxycoumarin (Dicumarol) and similar drugs is easily demonstrated. Any reduction in accessory factors (labile factor 3 or Owrens factor 5 4) is

A circulating anticoagulant in gamma-1A-multiple myeloma: its modification by penicillin.

“…The one-stage prothrombin was determined by the Quick assay (8), with rabbit brain thromboplastin.1 In one instance the thromboplastin was serially diluted 1/10, 1/100, and 1/1,000 with saline, and the prothrombin time then determined with the diluted thromboplastin. Prothrombin content was determined by the p-toluensulfonyl-l-arginine methyl ester (TAMe) synthetic substrate method (9) and prothrombin consumption by a modification of the * Submitted for publication March 5, 1965; accepted July 23, 1965. Presented in part at the Midwest Blood Club, Chicago, method of Sussman, Cohen, and Gittler (10). Exactly 1 hour after coagulation had occurred, the further conversion of prothrombin to thrombin was arrested by the addition of 0.2 ml of sodium citrate.…”

Blood Clotting Studies in a Submarine Crew

“…The PCT reveals platelet abnormalities before significant fall in platelet count is recognized. 8 However, at weeks 5 and 9 of the testing period Rumple-Leeds tourniquet tests were performed on each subject, and also at week 9 a blood smear was done. In each instance a negative result, or adequate level of platelets, respectively, was discovered.…”

“…The thrombocytopenias caused by some drugs are well documented. 8 Of more relevance to the Polaris environment is the tendency of salicylates to cause depression of vitamin K-dependent coagulation factors if ingested chronically and in undue amounts. 9 Aspirin and "AP.C' s" (acetylsalicylic acid, phenacetin, and caffeine) have long been handed out liberally in the submarine force.…”

An acquired inhibitor to factor VIIIC in a non‐hemophiliac: Twenty years of observation and characterization

“…The prothrombin time (PT) was measured by the method of Quick [2], the bleeding time by the Ivy method, [3] the clotting time (CT) by the three-tube Lee-White procedure [4], and the prothrombin consumption (pro consum) by the method of Sussman et a1 [5]. The non-activated partial thromboplastin time (PTT) was measured in the earlier periods without surface activation (PTT 50-80 sec) [6].…”