Clinical applications of assessment of airway inflammation using induced sputum

Pavord, Ian; Sterk, Peter J.; Hargreave, Frederick E.; Kips, Johan; Inman, Mark D.; Louis, Renaud; Pizzichini, Menezes; Bel, EH; Pin, I.; Grootendorst, Diana C.; Parameswaran, Krishnan; Djukanović, Ratko

doi:10.1183/09031936.02.00004002

Cited by 34 publications

(2 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A threshold of 50% viability has been proposed as a safe low limit to reliable results 13,14 . In our case, we must point out that we had a TCC < 50% in only one of the frozen spontaneous sputum samples, which is the cut-off suggested for reliability of the sputum cell measurements (9,13).…”

Section: Discussionmentioning

confidence: 85%

See 1 more Smart Citation

Freezing of sputum as a way to improve the applicability of sputum studies

Popov

Petlichkovski²,

Mustakov

et al. 2018

Biomedical Reviews

View full text Add to dashboard Cite

Rationale: within 2 hours of their collection poses limitations to its wider applicability. A way to bypass this hurdle would be to freeze the sample at the time of collection and to examine it at a later stage. Methods and Subjects: We developed a protocol for freezing of sputum upon collection by adding dimethylsulfoxide to it. We tested the reproducibility of cell counts in frozen samples and in fresh portions from the same sputum specimens. We took sputum from 41 asthmatics (18 males) with different levels of control of their disease: 19 of the samples were spontaneously produced and 22 were induced with hypertonic saline. Results: neutrophils, eosinophils and macrophages in the paired fresh and frozen sputum samples. Cell viability in frozen sputum was had better reproducibility than the spontaneous ones. Conclusion: Examination of frozen sputum samples does not change total cell counts and differential cell counts, despite conbut one examined specimens.

show abstract

Section: Discussionmentioning

confidence: 85%

“…Sputum induction and examination withstood the test of time and imposed itself as a valuable tool in clinical research (9,10). However, there are certain limitations to the wider applicability of sputum studies in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Freezing of sputum as a way to improve the applicability of sputum studies

Popov

Petlichkovski²,

Mustakov

et al. 2018

Biomedical Reviews

View full text Add to dashboard Cite

show abstract

Sputum Autoantibodies in Patients With Established Rheumatoid Arthritis and Subjects at Risk of Future Clinically Apparent Disease

Willis

Demoruelle

Derber

et al. 2013

Arthritis & Rheumatism

195

158

View full text Add to dashboard Cite

Objective To evaluate the generation of rheumatoid arthritis (RA)–related autoantibodies in the lung. Methods Simultaneous collection of serum and induced sputum was performed in 21 healthy controls, 49 at-risk subjects without inflammatory arthritis but at risk of RA due to family history or seropositivity for anti–citrullinated protein antibodies, and 14 subjects with early RA. Samples were tested for anti–cyclic citrullinated peptide 2 (anti-CCP2), anti-CCP3, anti-CCP3.1, rheumatoid factor isotypes IgM, IgG, and IgA, and total IgM, IgG, and IgA. Results One or more autoantibodies were present in sputum of 39% of at-risk seronegative subjects, 65% of at-risk seropositive subjects, and 86% of subjects with early RA. In at-risk seronegative subjects, the rate of anti-CCP3.1 positivity and the median number of autoantibodies were elevated in sputum versus serum. In subjects with early RA, the rate of positivity for several individual autoantibodies and the median number of autoantibodies were higher in serum than in sputum. Results in at-risk seropositive subjects were intermediate between these groups. In at-risk subjects with autoantibody positivity in sputum, the ratios of autoantibody to total Ig were higher in sputum than in serum, suggesting that these autoantibodies are generated or sequestered in the lung. Conclusion RA-related autoantibodies are detectable in sputum in subjects at risk of RA and in subjects with early RA. In a subset of at-risk subjects, the presence of sputum autoantibodies in the absence of seropositivity, and the increased autoantibody-to–total Ig ratios in sputum, suggest that the lung may be a site of autoantibody generation in the early development of RA. These findings suggest an important role of the lung in the pathogenesis of RA.

show abstract

Non-invasive Assessment of Airway Inflammation

Pavord

Kharitonov

2009

Asthma and COPD

View full text Add to dashboard Cite

Clinical applications of assessment of airway inflammation using induced sputum

Cited by 34 publications

References 35 publications

Freezing of sputum as a way to improve the applicability of sputum studies

Freezing of sputum as a way to improve the applicability of sputum studies

Sputum Autoantibodies in Patients With Established Rheumatoid Arthritis and Subjects at Risk of Future Clinically Apparent Disease

Non-invasive Assessment of Airway Inflammation

Contact Info

Product

Resources

About