Clinical assessment of serum myosin light chains in the diagnosis of acute myocardial infarction

Trahern, Charles A.; Gere, J. Brewster; Krauth, Gary H.; Bigham, Darlene A.

doi:10.1016/0002-9149(78)90811-1

Cited by 57 publications

(17 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, up to 30% of AAD patients remain undiagnosed until autopsy. 3 The biochemical methods for diagnosing acute myocardial infarction (AMI) have advanced greatly in the past quarter century; these methods include the use of creatinine phosphokinase (CK), the MB isozyme of CK, 4 cardiac myosin light chain, 5 troponin T, 6 and human heart fatty acid binding protein. 7,8 Meanwhile, for the diagnosis of AAD, smooth muscle myosin heavy chain 9,10 and C-reactive protein (CRP) 11 in the serum have both been reported to be such candidates.…”

Section: See Page 1721mentioning

confidence: 99%

Soluble Elastin Fragments in Serum Are Elevated in Acute Aortic Dissection

Shinohara

Suzuki

Okada

et al. 2003

ATVB

137

View full text Add to dashboard Cite

Objective-We aimed to establish an enzyme-linked immunosorbent assay for measuring soluble elastin fragments (sELAF) in serum and to reveal its usefulness in diagnosing acute aortic dissection (AAD). Methods and Results-An enzyme-linked immunosorbent assay to measure sELAF in serum was developed by using the newly created double monoclonal antibodies, which recognize the different epitopes of human aortic elastin. Twenty-five AAD patients, 50 patients with acute myocardial infarction (AMI), and 474 healthy individuals were enrolled in the study. The sELAF levels from healthy subjects gradually increased with aging. When the cutoff point for positivity was set at the meanϩ3 SD (ie, 3 SD above the mean in healthy subjects at each age), 16 AAD patients (64.0%) were found be positive, whereas only 1 AMI patient was found to be positive (2.0%). AAD patients with either an open or a partially open pseudolumen were found be 88.9% positive for sELAF, whereas those with its early closure were 0% positive. The difference in the sELAF levels between AAD patients with and without a thrombotic closure of false lumen was significant (60.3Ϯ15.6 versus 135.4Ϯ53.2 ng/mL, respectively; PϽ0.005). Key Words: matrix protein Ⅲ elastin degradation Ⅲ aortic media Ⅲ intimal tear Ⅲ false lumen A cute aortic dissection (AAD) is a life-threatening medical emergency of the aorta that is associated with a high mortality. In AAD patients who do not receive immediate treatment, mortality is estimated to be 35% within the initial 24 hours, 50% within 48 hours, and 80% after 2 weeks. 1 Conclusions-The

show abstract

Section: See Page 1721mentioning

confidence: 99%

Soluble Elastin Fragments in Serum Are Elevated in Acute Aortic Dissection

Shinohara

Suzuki

Okada

et al. 2003

ATVB

137

View full text Add to dashboard Cite

show abstract

“…HVMLC1 was not detectable in reference persons. However, the reference range varies considerably between different reports from <0.1 up to 6.6 pg/1 [1,3,11,12]. This may be due to a number of factors such as the calibration of the assay and the selected antibodies appearing after injection of HVMLC1 in the animal.…”

Section: Discussionmentioning

confidence: 99%

“…Special attention has been paid to myosin light chains (MLC), myosin heavy chains, tropomyosin, troponin T (TnT) and troponin I (Tnl) of the troponin-complex [1][2][3][4][5][6][7][8][9]. These assays have been developed to obtain more sensitive and specific markers of myocardial injury than with the conventional enzymes presently used, that is creatine kinase (CK), aspartate aminotransferase (ASAT), and lactate dehy drogenase (LD).…”

Section: Introductionmentioning

confidence: 99%

Human Ventricular Myosin Light Chain Isotype 1 as a Marker of Myocardial Injury

Ravkilde

Bøtker²,

Sogaard³

et al. 1994

Cardiology

View full text Add to dashboard Cite

A monoclonal enzyme immunoassay for measuring human ventricular myosin light chain isotype 1 (HVMLC1) in serum has been developed. To evaluate the method in patients with suspected myocardial injury, we studied 51 patients (16 acute myocardial infarction (AMI), 19 unstable angina pectoris (UAP), 9 stable angina pectoris, 3 nonischemic heart disease, 4 hip surgery patients), and 190 controls (blood donors). Serial blood-samples were drawn from patients; a single blood-sample from controls. The diagnostic value of the HVMLC1 assay was compared with total creatine kinase (CK), CKMB activity, CKMB mass concentration, lactate dehydrogenase isoenzyme 1 (LD1), troponin T (TnT) and mitochondrial-aspartate aminotransferase (m-ASAT). The detection limit of HVMLC1 was 0.4 µg/l (linear range 0-20 µg/l). Sera from 190 reference persons did not contain detectable levels of HVMLC1 ( < 0.4 µg/l; 99% percentile). The coefficients of variation were 13% (1.0 µg/l) and 3.1% (17.7 µg/l). Cross-reactivity with myosin from skeletal muscle was seen. Times to peak value were: CK 19.3 ± 2.0, LD1 43.4 ± 3.2, HVMLC1 72.9 ± 7.0, and m-ASAT 67.3 ± 5.6 h. Time-curves of HVMLC1 and m-ASAT were similar, whereas time-curves for HVMLC 1 and TnT were quite different in most cases. Peak value of HVMLC 1 was five times higher than CK peak value and eight times that of LD1. HVMLC 1 appeared in the blood within hours after the onset of chest pain and in the majority remained for more than a week after AMI. Among patients with UAP 16% (3/19) had elevated HVMLC1 in serum, whereas elevated TnT was seen in 26% (5/19) and elevated CKMB mass in 26% (5/19). We conclude that the new HVMLC 1 assay offers a sensitive diagnosis of myocardial injury. It is characterized by a wide diagnostic time window. The similarity of the HVMLC 1 and m-ASAT curves indicates that it may be used to estimate the extent of myocardial necrosis.

show abstract

“…electrocardiogram(ECG) and structural damage of cardiac myocytes and its sub cellular organelles with massive efflux of muscle enzymes viz creatine kinase (CK), Aspartate transaminase(AST), Lactate dehydrogenase (LDH) and Creatine kinase MB isoform (CK-MB). Late degradation of myofibrillar proteins myosin light chain (9,10,11) and regulatory proteins troponin T and troponin I (4 -24 hours) was demonstrated (12) and was attributed to action of Lysosomal enzymes Cathepsin D (13) and Cathepsin B (14). However, Srivastava et al (15,16) observed degradation of myofibrillar proteins as early as 30 to 60 min of total invitro ischemia in dog heart and troponin T and alpha actinin were the chief candidates.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of acute myocardial infarction: CK-MB versus cTn-T in Indian patients

Gupta

Singh

Bapat

et al. 2008

Indian J Clin Biochem

View full text Add to dashboard Cite

The comparative diagnostic efficacy of two cardiac markers : CK-MB and cTn-T , has scarcely been investigated in Indian patients of acute myocardial infarction. The present study was conducted for the same objective. The present study comprised of 59 patients . Males were 44 (75%) and females were 15 (25 %). The age of patients ranged from 32-84 years with mean age of 62.8 yrs. The mean age of males and females were 60 and 63 yrs respectively. All patients presented with history of chest pain with a 12 leads ECG proven MI (ST Elevation, discordant T-waves). CK-MB was estimated in peripheral blood samples at 0,24,48 and 72 hours by an autoanalyzer. Following 12 hours of admission bed side Troponin -T test was done employing cTn-T marker kit. Initially (0 hr), in 50% patients CK-MB was elevated. By end of 24 hours all the patients were CK-MB positive and peak level was attained at 24 hrs. Then it tended to decline over next 48 hrs. There were no false positive or negative results. The cTn-T test was positive only in 22 % of ECG positive infarctions. However, the cTn-T positive cases were always accompanied by a higher CK-MB levels. A significantly lower cTn-T positive cases in Indian patients can only be attributed to some difference in amino acid sequence of Indian cTn-T and occidental cTn-T. A larger study from other Indian cardiac centers can either substantiate or contradict our results.

show abstract

Clinical assessment of serum myosin light chains in the diagnosis of acute myocardial infarction

Cited by 57 publications

References 21 publications

Soluble Elastin Fragments in Serum Are Elevated in Acute Aortic Dissection

Soluble Elastin Fragments in Serum Are Elevated in Acute Aortic Dissection

Human Ventricular Myosin Light Chain Isotype 1 as a Marker of Myocardial Injury

Diagnosis of acute myocardial infarction: CK-MB versus cTn-T in Indian patients

Contact Info

Product

Resources

About